On-line analysis of penicillin blood levels in the live rat by combined microdialysis/fast-atom bombardment mass spectrometry.

Caprioli, Richard M.; Lin, Shen‐Nan

doi:10.1073/pnas.87.1.240

Cited by 60 publications

(9 citation statements)

References 5 publications

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“…For less accessible environments, such as in vivo , it is necessary to couple the mass spectrometer to a sampling probe. In one example, microdialysis was coupled with fast atom bombardment MS to monitor penicillin G in the bloodstream of a live rat; however, the sample was collected in an injection valve before transferring to the ionization source so that temporal resolution was limited by the time required to collect sufficient sample, which was 20 min 6 . Microdialysis sampling has also been coupled online with liquid chromatography (LC)-MS 7 ; but, sample collection and LC separation slow the overall process.…”

Section: Introductionmentioning

confidence: 99%

Mass Spectrometry “Sensor” forin VivoAcetylcholine Monitoring

Song¹,

Hershey²,

Mabrouk³

et al. 2012

Anal. Chem.

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Developing sensors for in vivo chemical monitoring is a daunting challenge. An alternative approach is to couple sampling methods with online analytical techniques; however, such approaches are generally hampered by lower temporal resolution and slow analysis. In this work, microdialysis sampling was coupled with segmented flow electrospray ionization mass spectrometry (ESI-MS) to perform in vivo chemical monitoring. Use of segmented flow to prevent Taylor dispersion of collected zones and rapid analysis with direct ESI-MS allowed 5 s temporal resolution to be achieved. The MS “sensor” was applied to monitoring acetylcholine in the brain of live rats. The detection limit of 5 nM was sufficient to monitor basal acetylcholine as well as dynamic changes elicited by microinjection of neostigmine, an inhibitor of acetycholinesterase that evoked rapid increases in acetycholine, and tetrodotoxin, a blocker of Na+ channels, that lowered the acetylcholine concentration. The versatility of the sensor was demonstrated by simultaneously monitoring metabolites and infused drugs.

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Section: Introductionmentioning

confidence: 99%

Mass Spectrometry “Sensor” forin VivoAcetylcholine Monitoring

Song¹,

Hershey²,

Mabrouk³

et al. 2012

Anal. Chem.

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“…An exciting and potentially important application was the coupling of mass spectrometers to microdialysis probes for the direct sampling of drugs and metabolites in living systems. The dialysate fluid from an indwelling microdialysis probe implanted in the jugular vein was allowed to flow into the mass spectrometer via an online CF FAB interface (Caprioli & Lin, 1990). Such online technology offered considerable promise for the conduct of metabolism and pharmacokinetic studies in real time.…”

Section: Early Phase Lc/msmentioning

confidence: 99%

Applications of mass spectrometry in drug metabolism: 50 years of progress

Wen

Zhu

2015

Drug Metabolism Reviews

109

102

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Mass spectrometry plays a pivotal role in drug metabolism studies, which are an integral part of drug discovery and development nowadays. Metabolite identification has become critical to understanding the metabolic fate of drug candidates and to aid lead optimization with improved metabolic stability, toxicology and efficacy profiles. Ever since the introduction of atmospheric ionization techniques in the early 1990s, liquid chromatography coupled with mass spectrometry (LC/MS) has secured a central role as the predominant analytical platform for metabolite identification as LC and MS technologies continually advanced. In this review, we discuss the evolution of both MS technology and its applications over the past 50 years to meet the increasing demand of drug metabolism studies. These advances include ionization sources, mass analyzers, a wide range of MS acquisition strategies and data mining tools that have substantially accelerated the metabolite identification process and changed the overall drug metabolism landscape. Exemplary applications for characterization and identification of both small-molecule xenobiotics and biological macromolecules are described. In addition, this review discusses novel MS technologies and applications, including xenobiotic metabolomics that hold additional promise for advancing drug metabolism research, and offers thoughts on remaining challenges in studying the metabolism and disposition of drugs and other xenobiotics.

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“…Early reports had used fast atom bombardment (FAB-MS) for ionization. 18) Nowadays, it is relatively convenient to couple the microdialysis probe to an ESI source. [19][20][21] For a solid or biological surface, extraction of analytes can be performed by the following methods.…”

Section: Remote Ambient Mass Spectrometrymentioning

confidence: 99%

Towards Practical Endoscopic Mass Spectrometry

et al. 2017

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In this paper, we brie y review the remote mass spectrometric techniques that are viable to perform "endoscopic mass spectrometry," i.e., in-situ and in-vivo MS analysis inside the cavity of human or animal body. We also report our experience with a moving string sampling probe for the remote sample collection and the transportation of adhered sample to an ion source near the mass spectrometer. With a miniaturization of the probe, the method described here has the potential to be t directly into a medical endoscope.

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On-line analysis of penicillin blood levels in the live rat by combined microdialysis/fast-atom bombardment mass spectrometry.

Cited by 60 publications

References 5 publications

Mass Spectrometry “Sensor” forin VivoAcetylcholine Monitoring

Mass Spectrometry “Sensor” forin VivoAcetylcholine Monitoring

Applications of mass spectrometry in drug metabolism: 50 years of progress

Towards Practical Endoscopic Mass Spectrometry

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