On‐line CE‐MS using pressurized liquid junction nanoflow electrospray interface and surface‐coated capillaries

Fanali, Salvatore; D’Orazio, Giovanni; Foret, František; Klepárnı́k, Karel; Aturki, Zeineb

doi:10.1002/elps.200600322

Cited by 49 publications

(46 citation statements)

References 33 publications

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“…Using cytochrome c tryptic digests, the authors could confirm the sensitivity improvement compared to the standard sheath flow interface, with LODs in the attomole range. More recently, Fanali et al [37] employed a liquid junction interface in which the flow of the spray liquid was hydrostatically induced by gravity from a small electrolyte reservoir. Under these conditions the CE-MS system generated an efficient, reproducible, and stable electrospray resulting in good sensitivity values that allowed the detection of ng/mL of peptides.…”

Section: Liquid Junction Interfacementioning

confidence: 99%

“…the electroosmotic flow, these neutral coatings are usually employed in CE-MS using a sheath flow interface, since, as already mentioned above, this interface is less dependant on the EOF. For instance, linear polyacrylamide has been used as coating for peptide analysis by CE-MS [67], as well as poly(vinyl alcohol) (PVA) [37,57,73,85] demonstrating its suitability to prevent peptides adsorption during the analysis of these molecules. In fact, Hu et al [73] showed that it was possible to identify using PVA-coated capillaries and CE-MS/ MS, common pathogen microorganisms in clinical diag- nosis by means of the selective identification of some of their specific peptides selected as biomarkers.…”

Section: Ce-ms Using Coated Capillariesmentioning

confidence: 99%

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Capillary electrophoresis‐electrospray‐mass spectrometry in peptide analysis and peptidomics

2008

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In the present work, an exhaustive review of the main developments and applications of CE-MS for peptide analysis is given. This review includes the use of different CE separation modes, MS analyzers, capillary coatings, preconcentration techniques, on-chip applications as well as other different multidimensional strategies for peptide analysis. Key applications are critically discussed and relevant works published from January 2000 to May 2007 are summarized including information concerning the type of sample, CE-MS parameters as well as some figures of merit of the different CE-MS procedures developed for peptide analysis and peptidomics.

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Section: Liquid Junction Interfacementioning

confidence: 99%

Section: Ce-ms Using Coated Capillariesmentioning

confidence: 99%

Capillary electrophoresis‐electrospray‐mass spectrometry in peptide analysis and peptidomics

2008

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“…In this work, we have expanded on the experience with the previously described CE-ESI interfaces based on the liquid junction [34][35][36]. The CE system including electrode chambers and separation capillary was integrated with the body of the pressurized liquid junction interface.…”

Section: Interface Setupmentioning

confidence: 99%

“…For example, the need for the use of electrospray compatible buffers limits the range of suitable BGE in the sheathless ESI interfacing. This limitation can be significantly decreased with the use of the liquid junction interface [33,34].…”

Section: Introductionmentioning

confidence: 98%

Optimization of a pressurized liquid junction nanoelectrospray interface between CE and MS for reliable proteomic analysis

Kusý

Klepárnı́k

Aturki³

et al. 2007

Electrophoresis

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A pressurized liquid junction nanoelectrospray interface was designed and optimized for reliable on-line CE-MS coupling. The system was constructed as an integrated device for highly sensitive and selective analyses of proteins and peptides with the separation and spray capillaries fixed in a pressurized spray liquid reservoir equipped with the electrode for connection of the electrospray potential. The electrode chamber on the injection side of the separation capillary and the spray liquid reservoir were pneumatically connected by a Teflon tube filled with pressurized nitrogen. This arrangement provided precisely counterbalanced pressures at the inlet and outlet of the separation capillary. The pressure control system was driven by an electrically operated valve and maintained the optimum flow rate for the electrospray stability. All parts of the interface being in contact with the CEBGE, spray liquid and/or sample were made of glass or Teflon. The use of these materials minimized the electrospray chemical noise often caused by plastic softeners or material degradation. During optimization, the transfer of the separated zones between the separation and electrospray capillaries was monitored by UV absorbance and contactless conductivity detectors placed at the outlet of the separation capillary and inlet of the electrospray tip, respectively. This arrangement allowed independent monitoring of the effects of pressure, CE voltage and geometry of the liquid junction on the spreading and dilution of the separated zones after passage through the interface.

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“…In general, commercially available devices require longer columns and spray emitters with consequent time- consuming analysis with a poor temperature control. Instead, the liquid junction laboratory device, optimized in previous studies [30,34], allows to work with shorter capillaries, thus reducing also band broadening. Several mobile phases composed by 20 mM ammonium formate buffer pH 3 and ACN in the range (20-50%) were tested and it was found that the mixture containing 30% ACN was suitable to ensure a high EOF and to resolve the analysed compounds.…”

Section: Cec With Uv Detectionmentioning

confidence: 99%

CEC‐ESI ion trap MS of multiple drugs of abuse

et al. 2010

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This article describes a method for the separation and determination of nine drugs of abuse in human urine, including amphetamines, cocaine, codeine, heroin and morphine. This method was based on SPE on a strong cation exchange cartridge followed by CEC-MS. The CEC experiments were performed in fused silica capillaries (100 mm Â 30 cm) packed with a 3 mm cyano derivatized silica stationary phase. A laboratory-made liquid junction interface was used for CEC-MS coupling. The outlet capillary column was connected with an emitter tip that was positioned in front of the MS orifice. A stable electrospray was produced at nanoliter per minute flow rates applying a hydrostatic pressure (few kPa) to the interface. The coupling of packed CEC columns with mass spectrometer as detector, using a liquid junction interface, provided several advantages such as better sensitivity, low dead volume and independent control of the conditions used for CEC separation and ESI analysis. For this purpose, preliminary experiments were carried out in CEC-UV to optimize the proper mobile phase for CEC analysis. Good separation efficiency was achieved for almost all compounds, using a mixture containing ACN and 25 mM ammonium formate buffer at pH 3 (30:70, v/v), as mobile phase and applying a voltage of 12 kV. ESI ion-trap MS detection was performed in the positive ionization mode. A spray liquid, composed by methanol-water (80:20, v/v) and 1% formic acid, was delivered at a nano-flow rate of $200 nL/min. Under optimized CEC-ESI-MS conditions, separation of the investigated drugs was performed within 13 min. CEC-MS and CEC-MS 2 spectra were obtained by providing the unambiguous confirmation of these drugs in urine samples. Method precision was determined with RSDs values r3.3% for retention times and r16.3% for peak areas in both intra-day and day-to-day experiments. LODs were established between 0.78 and 3.12 ng/mL for all compounds. Linearity was satisfactory in the concentration range of interest for all compounds (r 2 Z0.995). The developed CEC-MS method was then applied to the analysis of drugs of abuse in spiked urine samples, obtaining recovery data in the range 80-95%.

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On‐line CE‐MS using pressurized liquid junction nanoflow electrospray interface and surface‐coated capillaries

Cited by 49 publications

References 33 publications

Capillary electrophoresis‐electrospray‐mass spectrometry in peptide analysis and peptidomics

Capillary electrophoresis‐electrospray‐mass spectrometry in peptide analysis and peptidomics

Optimization of a pressurized liquid junction nanoelectrospray interface between CE and MS for reliable proteomic analysis

CEC‐ESI ion trap MS of multiple drugs of abuse

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