On-line flow sample stacking in a flow injection analysis–capillary electrophoresis system: 2000-fold enhancement of detection sensitivity for priority phenol pollutants

Kubáň, Petr; Berg, M.A.M.C. van den; Garcı́a, Carlos D.; Karlberg, Bo

doi:10.1016/s0021-9673(01)00549-0

Cited by 56 publications

(23 citation statements)

References 21 publications

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“…However, capillary electrophoresis has the disadvantage that the injection volumes are very small. To overcome this, several injection techniques have been developed [25][26][27][28]. One technique to enhance the sensitivity of detection is on-line sample concentration by sample stacking with reversed polarity [29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Determination of Ochratoxin A in small volumes of human blood serum

Köller

Rolle-Kampczyk

Lehmann

et al. 2004

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Determination of Ochratoxin A in small volumes of human blood serum

Köller

Rolle-Kampczyk

Lehmann

et al. 2004

Journal of Chromatography B

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“…A specific recent report utilized [89] this approach to determine peptides in the low femtomole range by capillary electrophoresis-tandem mass spectrometry for routine application in proteomic studies. A 2000-fold enhancement of detection sensitivity was reported [90] for priority phenol pollutants using sample stacking at high pH, where the phenols are ionised.…”

Section: Sensitivity Improvementsmentioning

confidence: 99%

Overview of the status and applications of capillary electrophoresis to the analysis of small molecules

Altria

Elder²

2004

Journal of Chromatography A

122

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“…One possible solution was provided in [19] implementing an intermediate trapping of the effluent with a short reversed-phase column. Stacking of the analytes could also be successfully implemented, especially in the dynamic mode for which a pneumatic sampler is well suited as demonstrated in [31,32] by improving the detection limits by three orders compared to the common CE analysis. This will be the subject of further studies.…”

Section: Comprehensive Hplc-ce Analysis Of Model and Real Samplementioning

confidence: 99%

Stroboscopic sampling in comprehensive high‐performance liquid chromatography‐capillary electrophoresis via a pneumatic sampler

et al. 2004

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A new approach is presented to solve the problem of a long separation time in the second dimension of comprehensive two-dimensional chromatography. The need for a rapid separation in the second column is overcome by repeating analysis of a sample many times. In each of these individual analysis cases the sample is injected into the first dimension column and after a delay a low amount of the effluent at the end of the first column is sampled to the second-dimensional column. The time interval between the samplings from the first column to the second column is constantly increased. Thus, the system enables a comprehensive analysis of the effluent emerging from the first into the second column. This approach, which we call stroboscopic sampling, is tested for coupling high-performance liquid chromatography (HPLC) to capillary electrophoresis (CE) by an interface which operates on the principle of transporting the effluent from the HPLC column to the capillary inlet by small pressure pulses (0.5 MPa). The performance of the interface for accomplishing the comprehensive HPLC-CE analysis was demonstrated for an on-line connection of a short ion-exchange column and an ion-exclusion column to the CE capillary.

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On-line flow sample stacking in a flow injection analysis–capillary electrophoresis system: 2000-fold enhancement of detection sensitivity for priority phenol pollutants

Cited by 56 publications

References 21 publications

Determination of Ochratoxin A in small volumes of human blood serum

Determination of Ochratoxin A in small volumes of human blood serum

Overview of the status and applications of capillary electrophoresis to the analysis of small molecules

Stroboscopic sampling in comprehensive high‐performance liquid chromatography‐capillary electrophoresis via a pneumatic sampler

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