On-line focusing of 5-hydroxy-tryptamine type 3 receptor antagonists via the combination of field-enhanced sample injection and dynamic pH junction in capillary electrophoresis with amperometric detection

Li, Libo; Xu, Lei; Huang, Jianshe; You, Tianyan

doi:10.1016/j.chroma.2014.01.028

Cited by 7 publications

(3 citation statements)

References 43 publications

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“…Previous studies reported many methods, such as radioimmunoassay, 14 spectrophotometric, 15 HPLC–UV, 16 high-performance thin layer chromatography, 17 and capillary electrophoresis, 18 to determine the plasma concentrations of ondansetron. However, these methods have some drawbacks such as laborious sample preparation procedures, time-consuming gradient elution, lower sensitivity, and comparatively longer runtime, which limited their application to large amounts of quantification in biological fluids.…”

Section: Discussionmentioning

confidence: 99%

Pharmacokinetics and bioavailability study of two ondansetron oral soluble film formulations in fasting healthy male Chinese volunteers

Zhu¹,

Zhang²,

Zou³

et al. 2015

DDDT

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BackgroundOndansetron oral soluble film is designed to be applied on top of the tongue without requiring water to aid dissolution or swallowing, which is especially fitting for nausea and vomiting patients.PurposeThis study was conducted to compare the bioavailability of two 8 mg ondansetron oral soluble film formulations.Patients and methodsThis randomized, open-label, two-period crossover study was performed under fasting conditions. A total of ten eligible subjects were randomly assigned at a 1:1 ratio to receive a single 8 mg dose of the test and reference ondansetron oral soluble film formulations, followed by a 1-week washout period and administration of the alternate formulation. The concentrations of ondansetron were assayed using an liquid chromatograph-mass spectrometer/mass spectrometer (LC-MS/MS) method. For analysis of pharmacokinetic properties, including the peak concentration of Tmax (Cmax), AUC from time 0 (baseline) to t hours (AUC0–t), and AUC from baseline to infinity (AUC0–∞), blood samples were obtained at intervals over the 24-hour period after studying drug administration. Tolerability was assessed by monitoring vital signs and laboratory tests (hematology, blood biochemistry, hepatic function, and urinalysis) and by questioning subjects about adverse events.ResultsThe mean (standard derivation [SD]) relative bioavailability was 96.5 (23.7%). The 90% confidence intervals (CIs) for the log-transformed ratios of Cmax and AUC0–t were 84.71%–103.28% and 91.38%–108.60%, respectively (P>0.05). Similar results were found for the data without log-transformation. No statistically significant differences were found based on analysis of variance. No significant adverse events occurred or were reported during the study.ConclusionAs the 90% CIs based on the differences between the test and reference formulation were within the 80%–125% range for both the Cmax and AUC0–t, we concluded that the two formulations were bioequivalent with respect to the rate or the extent of absorption. Both formulations are well tolerated.

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Section: Discussionmentioning

confidence: 99%

Pharmacokinetics and bioavailability study of two ondansetron oral soluble film formulations in fasting healthy male Chinese volunteers

Zhu¹,

Zhang²,

Zou³

et al. 2015

DDDT

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“…Li et al. published a method for the analysis of two 5‐hydroxy‐tryptamine type 3 receptor antagonists ondansetron and tropisetron in human urine by CE with amperometric detection. By using 30 mM sodium phosphate buffer at pH 9.0 as the BGE and by applying the sample diluted 20 times with 0.1 mM H 3 PO 4 (pH 4.0), both FASI and pH‐mediated stacking could be used for the two analytes that are medium strong bases with p K a values 7.4 and 8.9, respectively.…”

Section: Ph‐mediated Stackingmentioning

confidence: 99%

Contemporary sample stacking in analytical electrophoresis

et al. 2014

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This contribution is a methodological review of the publications about the topic from the last 2 years. Therefore, it is primarily organized according to the methods and procedures used in surveyed papers and the origin and type of sample and specification of analytes form the secondary structure. The introductory part about navigation in the architecture of stacking brings a brief characterization of the various stacking methods, with the description of mutual links to each other and important differences among them. The main body of the article brings a survey of publications organized according to main principles of stacking and then according to the origin and type of the sample. Provided that the paper cited gave explicitly the relevant data, information about the BGE(s) used, procedure, detector employed, and reached LOD and/or concentration effect is given. The papers where the procedure used is a combination of diverse fragments and parts of various stacking techniques are mentioned in a special section on combined techniques. The concluding remarks in the final part of the review evaluate present state of art and the trends of sample stacking in CE.

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“…In addition, electrokinetic injection is used instead of hydrodynamic injection for better preconcentration potential through sample stacking. FASI is the most simple and effective concentration technique that commonly used for stacking inorganic ions or ionic organic compounds, and has been widely used for concentrating organic pollutants , receptor antagonist , and drugs .…”

Section: Introductionmentioning

confidence: 99%

Membrane supported liquid-liquid-liquid microextraction combined with field-amplified sample injection CE-UV for high-sensitivity analysis of six cardiovascular drugs in human urine sample

Zhou

Chen

et al. 2016

ELECTROPHORESIS

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An effective dual preconcentration method involving off-line membrane supported liquid-liquid-liquid microextraction (MS-LLLME) and on-line field-amplified sample injection (FASI) was proposed for the extraction of six cardiovascular drugs, including mexiletine, xylocaine, propafenone, propranolol, metoprolol, and carvedilol from aqueous solution prior to CE-UV. In MS-LLLME, the analytes were extracted from 9 mL sample solution into toluene, and then back extracted into a drop of acceptor phase of 10 μL 20 mmol/L acetic acid. After that, the acceptor phase was directly introduced into CE for FASI without any modification. In FASI process, water plug was hydrodynamically injected (50 mbar, 3 s) into the capillary prior to sample injection (+6 kV, 18 s). Six target analytes were separated in less than 10 min at 25°C with a BGE consisting of 70 mmol/L Tris-H3 PO4 (pH 2.2) containing 10% v/v methanol. Under the optimized conditions, LODs obtained by the proposed MS-LLLME-FASI-CE-UV method were in the range of 0.02-0.82 μg/L (based on S/N = 3) with enrichment factors of 546- to 7300-fold for the target analytes. The RSDs of the developed method were in the range of 6.7-12.9% (n = 7). Good linearity (R(2) = 0.9928-0.9997) was obtained in concentration range of 0.1-100 μg/L for mexiletine and propranolol, 0.2-100 μg/L for xylocaine and metoprolol, 0.5-100 μg/L for propafenone and 2.0-100 μg/L for carvedilol, respectively. The developed method was successfully applied for real-time determination of metoprolol in human urine samples within 26 h after uptake.

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On-line focusing of 5-hydroxy-tryptamine type 3 receptor antagonists via the combination of field-enhanced sample injection and dynamic pH junction in capillary electrophoresis with amperometric detection

Cited by 7 publications

References 43 publications

Pharmacokinetics and bioavailability study of two ondansetron oral soluble film formulations in fasting healthy male Chinese volunteers

Pharmacokinetics and bioavailability study of two ondansetron oral soluble film formulations in fasting healthy male Chinese volunteers

Contemporary sample stacking in analytical electrophoresis

Membrane supported liquid-liquid-liquid microextraction combined with field-amplified sample injection CE-UV for high-sensitivity analysis of six cardiovascular drugs in human urine sample

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On-line focusing of 5-hydroxy-tryptamine type 3 receptor antagonists via the combination of field-enhanced sample injection and dynamic pH junction in capillary electrophoresis with amperometric detection

Cited by 7 publications

References 43 publications

Pharmacokinetics and bioavailability study of two ondansetron oral soluble film formulations in&nbsp;fasting healthy male Chinese volunteers

Pharmacokinetics and bioavailability study of two ondansetron oral soluble film formulations in&nbsp;fasting healthy male Chinese volunteers

Contemporary sample stacking in analytical electrophoresis

Membrane supported liquid-liquid-liquid microextraction combined with field-amplified sample injection CE-UV for high-sensitivity analysis of six cardiovascular drugs in human urine sample

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Pharmacokinetics and bioavailability study of two ondansetron oral soluble film formulations in fasting healthy male Chinese volunteers

Pharmacokinetics and bioavailability study of two ondansetron oral soluble film formulations in fasting healthy male Chinese volunteers