On-Line Immunoaffinity Extraction and HPLC Analysis of Flunitrazepam and Its Main Metabolites in Serum

Deinl, Ingo; Franzelius, Cornelia; Angermaier, L; Mahr, G.; Machbert, G.

doi:10.1093/jat/23.7.598

Cited by 9 publications

(4 citation statements)

References 16 publications

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“…On-line immunoaffinity chromatography coupled with RP chromatography and a radioimmunoassay was developed for analyzing 4-[(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-naphthyl)-carbamoyl] benzoic acid in human plasma [222]. Another study reported an on-line immunoextraction method for the determination of flunitrazepam and its major metabolites in serum and plasma [223].…”

Section: Biointeraction Studiesmentioning

confidence: 99%

Applications of silica supports in affinity chromatography

Schiel

Mallik

Soman

et al. 2006

J of Separation Science

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The combined use of silica-based chromatographic supports with immobilized affinity ligands can be used in many preparative and analytical applications. One example is the use of silica-based affinity columns in HPLC, giving rise to a method known as high-performance affinity chromatography (HPAC). This review discusses the role that silica has played in the development of affinity chromatography and HPAC and the applications of silica in these methods. This includes a discussion of the types of ligands that have been employed with silica and the methods by which these ligands have been immobilized. Various formats have also been presented for the use of silica in affinity chromatographic methods, including assays involving direct or indirect analyte detection, on-line or off-line affinity extraction, and chiral separations. The use of silica-based affinity columns in studies of biological systems based on zonal elution and frontal analysis methods will also be considered.

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Section: Biointeraction Studiesmentioning

confidence: 99%

Applications of silica supports in affinity chromatography

Schiel

Mallik

Soman

et al. 2006

J of Separation Science

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“…Senstivities of the methods are typically not better than 1 ng/mL. To address these issues, a column-switching method employing an anti-benzodiazepine immunoaffinity column for on-line sample pre-treatment has been reported [21]. By this method sample pre-treatment and analysis was automated, total analysis time was under 40 min and the reported limit of detection was 1 ng/mL.…”

Section: Requirements For Flunitrazepam Analysismentioning

confidence: 99%

Development of immunoaffinity solid phase microextraction probes for analysis of sub ng/mL concentrations of 7-aminoflunitrazepam in urine

Lord

Rajabi

Safari

et al. 2006

Journal of Pharmaceutical and Biomedical Analysis

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“…This technique has already been successfully applied for on-line or separate purification and extraction in several cases [22][23][24], e.g. for the extraction of estradiol [25], clenbuterol [26], amanitin [27], tetrahydrocannabinol [28,29], benzodiazepines and their metabolites [30,31], LSD [32,33] and morphine and its metabolites [34,35]. We developed a new immunoadsorber for the extraction of morphine and its glucuronides by the immobilisation of specific antibodies against morphine and morphine glucuronides [36] applying the solgel method.…”

Section: Introductionmentioning

confidence: 99%

HPLC with laser-induced native fluorescence detection for morphine and morphine glucuronides from blood after immunoaffinity extraction

et al. 2005

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A new immunoaffinity solid phase extraction of morphine and its phase II metabolites, morphine-3-beta-D-glucuronide and morphine-6-beta-D-glucuronide is described. An immunoadsorber was applied which was created for the first time by the immobilisation of specific antibodies (polyclonal, host: rabbit) by the sol-gel method. The extraction method in combination with high performance liquid chromatography-fluorescence determination has been validated and shown to be applicable to blood samples of heroin victims in a low concentration range. Blood extracts were essentially free of interfering matrix components when compared to C8-extracts. Additionally, a novel, sensitive and selective detection system for wavelength-resolved analysis of laser-induced fluorescence coupled to HPLC was developed. The analytes were excited with a frequency tripled Ti:Sa laser (lambda=244 nm quasi cw). The total emission spectrum was recorded with a detection system consisting of an imaging spectrograph and a back-illuminated CCD camera. This technique of detection, combined with an extended optical path (at least 6 mm could be illuminated by the laser), resulted in an optimal fluorescence intensity of the analytes. The method permitted the analysis of morphine, morphine-3-beta-D-glucuronide and morphine-6-beta-D-glucuronide in a low concentration range and could be applied to a complex matrix such as postmortem blood samples because analyte peaks could be discriminated from matrix peaks by their characteristic emission spectra.

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On-Line Immunoaffinity Extraction and HPLC Analysis of Flunitrazepam and Its Main Metabolites in Serum

Cited by 9 publications

References 16 publications

Applications of silica supports in affinity chromatography

Applications of silica supports in affinity chromatography

Development of immunoaffinity solid phase microextraction probes for analysis of sub ng/mL concentrations of 7-aminoflunitrazepam in urine

HPLC with laser-induced native fluorescence detection for morphine and morphine glucuronides from blood after immunoaffinity extraction

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