On-line micellar electrokinetic chromatography–mass spectrometry: feasibility of direct introduction of non-volatile buffer and surfactant into the electrospray interface

Somsen, Govert W.; Mol, Roelof; Jong, Gerhardus J. de

doi:10.1016/s0021-9673(03)00179-1

Cited by 66 publications

(56 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Taking this into account, it can be concluded that no, or hardly any, ionization suppression occurs when the nonvolatile BGE is employed. This shows that APCI is clearly more resistant towards nonvolatile BGE constituents than ESI [2,8,19].…”

Section: Ce-apci-msmentioning

confidence: 92%

“…In sharp contrast to these APCI results, the efficiency of ESI is known to decrease significantly in the presence of nonvolatile constituents such as sodium phosphate [2,8]. SDS, which is often employed as a pseudo-stationary phase in micellar electrokinetic chromatography (MEKC), is an even stronger ion suppressor in ESI [18,19]. However, the infusion experiments indicated that the addition of 20 mM SDS did not result in reduced signal intensities in APCI-MS. For hydrocortisone, SDS even caused a slight signal increase.…”

Section: Bge Compositionmentioning

confidence: 99%

See 1 more Smart Citation

Capillary electrophoresis-atmospheric pressure chemical ionization-mass spectrometry using an orthogonal interface: Set-up and system parameters

Hommerson

Khan

Jong

et al. 2009

J. Am. Soc. Mass Spectrom.

Self Cite

View full text Add to dashboard Cite

The feasibility of atmospheric pressure chemical ionization (APCI) as an alternative ionization technique for capillary electrophoresis-mass spectrometry (CE-MS) was investigated using a grounded sheath-flow CE-MS sprayer and an orthogonal APCI source. Infusion experiments indicated that highest analyte signals were achieved when the sprayer tip was in close vicinity of the vaporizer entrance. The APCI-MS set-up enabled detection of basic, neutral, and acidic compounds, whereas apolar and ionic compounds could not be detected. In the positive ion mode, analytes could be detected in the entire transfer voltage range (0 -5 kV), whereas highest signal intensities were observed when the corona discharge current was between 1000 and 2000 nA. In the negative ion mode, the transfer voltage typically was 500 V and the optimum corona discharge current was 6000 nA. Analyte signals were raised with increasing nebulizing gas pressure, but the pressure was limited to 25 psi to avoid siphoning and current drops. Signal intensities appeared to be optimal and constant over a wide range of sheath liquid flow rate (5-25 L/min) and vaporizer temperature (200 -350°C). APCI-MS signals were unaffected by the composition of the background electrolyte (BGE), even when it contained sodium phosphate and sodium dodecyl sulfate (SDS). Consequently, BGE composition, sheath-liquid flow rate, and vaporizer temperature can be optimized with respect to the CE separation without affecting the APCI-MS response. The analysis of a mixture of basic compounds and a steroid using volatile and nonvolatile BGEs further demonstrates the feasibility of CE-APCI-MS. Detection limits (S/N ϭ 3) were 1.6 -10 M injected concentrations. (J Am Soc Mass

show abstract

Section: Ce-apci-msmentioning

confidence: 92%

Section: Bge Compositionmentioning

confidence: 99%

Capillary electrophoresis-atmospheric pressure chemical ionization-mass spectrometry using an orthogonal interface: Set-up and system parameters

Hommerson

Khan

Jong

et al. 2009

J. Am. Soc. Mass Spectrom.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Indeed, common buffers used in CE-UV such as phosphate or borate which provide good peak shapes and thus high separation efficiency are nonvolatile and/or can contain modifiers such as CD, SDS, and so on. These additives are not suitable or even detrimental for on-line CE-ESI/MS since they may contaminate the MS ionization chamber and cause significant ion suppression [18,19]. Numerous solutions have been proposed to overcome these difficulties, such as the partial filling approach in chiral analysis [20], but they are not always well adapted or easy to carry out.…”

Section: Introductionmentioning

confidence: 99%

Coupling CE with atmospheric pressure photoionization MS for pharmaceutical basic compounds: Optimization of operating parameters

Schappler¹,

Guillarme²,

Prat³

et al. 2007

Electrophoresis

View full text Add to dashboard Cite

The use of CE coupled with MS (CE-MS) has evolved as a useful tool to analyze charged species in small sample volumes. Because of its sensitivity, versatility and ease of implementation, the ESI interface is currently the method of choice to hyphenate CE to MS. An alternative can be the atmospheric pressure photoionization (APPI) source, however, numerous parameters must be optimized for its coupling to CE. After evaluation of the sheath liquid composition and the CE capillary outlet position, an experimental design methodology was assessed for optimizing other ionization source parameters, such as sheath liquid flow rate, drying gas flow rate and temperature, nebulizing gas pressure, vaporizer temperature, and capillary voltage. For this purpose, a fractional factorial design (FFD) was selected as a screening procedure to identify factors which significantly influence sensitivity and efficiency. A face-centered central composite design (CCD) was then used to predict and optimize sensitivity, taking into account the most relevant variables. Sensitivity was finally evaluated with the optimized conditions and height-to-noise ratios (H/N) around 10 were achieved for an injection of 200 ng/mL of each analyte.

show abstract

“…The reported methods for its determination are chromatographic, characterized by their good sensitivities. They include high performance liquid chromatography (Hoogewijs and Massart, 1986;Stockis et al, 2002), on-line micellar electrokinetic chromatography-mass spectrometry (Somsen et al, 2003), high-performance thin-layer chromatography (De Schutter et al, 1985;El-Walily et al, 1991), capillary supercritical fluid chromatography-mass spectrometry (Pinkston et al, 1993), gas chromatography-mass spectrometry, and online reversed-phase liquid chromatography-gas chromatography (Kristinsson et al, 1994;Tulich et al, 1996;Kraemer et al, 2000). Other alternatives include spectrophotometry (El-Walily et al, 1991;Shama and Amin, 2004) and potentiometric flow injection analysis (Ibrahim et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

A validated chiral HPLC method for the determination of mebeverine HCl enantiomers in pharmaceutical dosage forms and spiked rat plasma

Radwan

Abdine

Aboul‐Enein

2005

Biomedical Chromatography

View full text Add to dashboard Cite

A new, precise, simple and accurate HPLC method was developed for the first time to separate and determine mebeverine enantiomers. Enantiomeric resolution was achieved on a cellulose Tris (3,5-dimethylphenyl carbamate) column known as Chiralcel OD, with UV detection at 263 nm. The mobile phase consisted of n-hexane, isopropyl alcohol and triethylamine (90:9.9:0.1 v/v/v). Sample run time was 18 min. On using the chromatographic conditions described, mebeverine enantiomers were well resolved with mean retention times of about 11 and 14 min. A linear response (r > 0.999) was observed over the concentration range 0.5-20 µg/mL racemic mebeverine. Precision, accuracy and stability were studied according to ICH guidelines. The limit of detection was found to be 0.05 µg/mL for each enantiomer of mebeverine. The proposed method was applied for analysis of mebeverine in commercially available tablets dosage formulations. Examples of application to biological samples are also given. Reanalysis of samples several weeks after the initial analysis showed no degradation of mebeverine. Copyright

show abstract

On-line micellar electrokinetic chromatography–mass spectrometry: feasibility of direct introduction of non-volatile buffer and surfactant into the electrospray interface

Cited by 66 publications

References 24 publications

Capillary electrophoresis-atmospheric pressure chemical ionization-mass spectrometry using an orthogonal interface: Set-up and system parameters

Capillary electrophoresis-atmospheric pressure chemical ionization-mass spectrometry using an orthogonal interface: Set-up and system parameters

Coupling CE with atmospheric pressure photoionization MS for pharmaceutical basic compounds: Optimization of operating parameters

A validated chiral HPLC method for the determination of mebeverine HCl enantiomers in pharmaceutical dosage forms and spiked rat plasma

Contact Info

Product

Resources

About