On‐line monitoring of bioreductions via membrane introduction mass spectrometry

Milagre, Cíntia D. F.; Milagre, Humberto M. S.; Rodrigues, J. Augusto R.; Rocha, Lilian Lúcia da; Santos, Leonardo S.; Eberlin, Marcos N.

doi:10.1002/bit.20472

Cited by 9 publications

(2 citation statements)

References 26 publications

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“…Because of that, the composition of electrosprayed ions usually reflects that in solution 21–24. Furthermore, these desirable characteristics have allowed ESI‐MS to be successfully employed as an important analytical tool for real‐time monitoring of a great variety of chemical reactions 25–27…”

Section: Introductionmentioning

confidence: 99%

Electrospray ionization mass spectrometry monitoring of indigo carmine degradation by advanced oxidative processes

et al. 2007

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The degradation of the dye indigo carmine in aqueous solution induced by two oxidative processes (H(2)O(2)/iodide and O(3)) was investigated. The reactions were monitored by electrospray ionization mass spectrometry in the negative ion mode, ESI(-)-MS, and the intermediates and oxidation products characterized by ESI(-)-MS/MS. Both oxidative systems showed to be highly efficient in removing the color of the dye aqueous solutions. In the ESI(-)-MS of the indigo carmine solution treated with H(2)O(2) and H(2)O(2)/iodide, the presence of the ions of m/z 210 (indigo carmine in its anionic form, 1), 216, 226, 235, and 244 was noticeable. The anion of m/z 235 was proposed to be the unprecedented hydroperoxide intermediate 2 formed in solution via an electrophilic attack by hydroxyl and hydroperoxyl radicals of the exocyclic C=C bond of 1. This intermediate was suggested to be rapidly converted into the anionic forms of 2,3-dioxo-1H-indole-5-sulfonic acid (3, m/z 226), 2-amino-alpha-oxo-5-sulfo-benzeneacetic acid (4, m/z 244), and 2-amino-5-sulfo-benzoic acid (5, m/z 216). In the ESI(-)-MS of the indigo carmine solution treated with O(3), two main anions were detected: m/z 216 (5) and 244 (4). Both products were proposed to be produced via an unstable ozonide intermediate. Other anions in this ESI(-) mass spectrum were attributed to be [4 - H + Na](-) of m/z 266, [4 - H](2-) of m/z 121.5, and [5 - H](2-) of m/z 107.5. ESI-MS/MS data were consistent with the proposed structures for the anionic products 2-5.

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Section: Introductionmentioning

confidence: 99%

Electrospray ionization mass spectrometry monitoring of indigo carmine degradation by advanced oxidative processes

et al. 2007

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“…The classic application of MIMS in a bioreactor is the monitoring of yeast fermentation. By immobilizing the biocatalyst onto alginate beads, reactants, intermediates, and final products for dicarbony, compound bioreductions were continuously monitored without leakage from the reactor or the consequent obstruction of the MIMS probe (Milagre et al, 2005). As shown in Figure 7B, chloroform formation during the chlorination of organic molecules was studied via online MIMS, which demonstrated reaction rates and kinetics of important species.…”

Section: Condensed-phase Reactionsmentioning

confidence: 99%

Chemical Reaction Monitoring by Ambient Mass Spectrometry

Sun

Yin

et al. 2020

Mass Spectrometry Reviews

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Chemical reactions conducted in different media (liquid phase, gas phase, or surface) drive developments of versatile techniques for the detection of intermediates and prediction of reasonable reaction pathways. Without sample pretreatment, ambient mass spectrometry (AMS) has been applied to obtain structural information of reactive molecules that differ in polarity and molecular weight. Commercial ion sources (e.g., electrospray ionization, atmospheric pressure chemical ionization, and direct analysis in real-time) have been reported to monitor substrates and products by offline reaction examination. While the interception or characterization of reactive intermediates with short lifetime are still limited by the offline modes. Notably, online ionization technologies, with high tolerance to salt, buffer, and pH, can achieve direct sampling and ionization of ongoing reactions conducted in different media (e.g., liquid phase, gas phase, or surface). Therefore, short-lived intermediates could be captured at unprecedented timescales, and the reaction dynamics could be studied for mechanism examinations without sample pretreatments. In this review, via various AMS methods, chemical reaction monitoring and mechanism elucidation for different classifications of reactions have been reviewed. The developments and advances of common ionization methods for offline reaction monitoring will also be highlighted.

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Current literature in mass spectrometry

2006

J. Mass Spectrom.

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In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of mass spectrometry. Each bibliography is divided into 11 sections: 1 Books, Reviews & Symposia; 2 Instrumental Techniques & Methods; 3 Gas Phase Ion Chemistry; 4 Biology/Biochemistry: Amino Acids, Peptides & Proteins; Carbohydrates; Lipids; Nucleic Acids; 5 Pharmacology/Toxicology; 6 Natural Products; 7 Analysis of Organic Compounds; 8 Analysis of Inorganics/Organometallics; 9 Surface Analysis; 10 Environmental Analysis; 11 Elemental Analysis. Within each section, articles are listed in alphabetical order with respect to author (4 Weeks journals ‐ Search completed at 2nd. Nov. 2005)

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On‐line monitoring of bioreductions via membrane introduction mass spectrometry

Cited by 9 publications

References 26 publications

Electrospray ionization mass spectrometry monitoring of indigo carmine degradation by advanced oxidative processes

Electrospray ionization mass spectrometry monitoring of indigo carmine degradation by advanced oxidative processes

Chemical Reaction Monitoring by Ambient Mass Spectrometry

Current literature in mass spectrometry

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