On-line monitoring of the dopamine-based molecular imprinting processes for protein templates with the assistance of a fluorescent indicator

Surface imprinted bio‐nanocomposites for affinity separation of a cellular DNA repair protein

“…[ 17 ] Such inhibition effects on the deposition of pDA have also been reported for several other proteins. [ 23–26 ] Recently, our group has developed a simple method for real‐time monitoring of the dopamine polymerization by using rhodamine B (RhB) as a fluorescent indicator. [ 26 ]…”

“…[ 23–26 ] Recently, our group has developed a simple method for real‐time monitoring of the dopamine polymerization by using rhodamine B (RhB) as a fluorescent indicator. [ 26 ]…”

“…[17] Such inhibition effects on the deposition of pDA have also been reported for several other proteins. [23][24][25][26] Recently, our group has developed a simple method for real-time monitoring of the dopamine polymerization by using rhodamine B (RhB) as a fluorescent indicator. [26] Due to the high binding affinity of pDA to RhB and the resultant quenching of the fluorescence of RhB, the deceleration effect of proteins on the polymerization reaction could be clearly seen via the fluorescence curves (Figure S1).…”

“…[23][24][25][26] Recently, our group has developed a simple method for real-time monitoring of the dopamine polymerization by using rhodamine B (RhB) as a fluorescent indicator. [26] Due to the high binding affinity of pDA to RhB and the resultant quenching of the fluorescence of RhB, the deceleration effect of proteins on the polymerization reaction could be clearly seen via the fluorescence curves (Figure S1). To mitigate such inhibition effects and incorporate more functional monomers, we tested to modify the glycosyl residues on the surface of AVD by adding aryl boronic acid groups.…”

Tetrabutylammonium-chloride-glycerol of deep eutectic solvent functionalized MnO2: a novel mimic enzyme for the quantitative and qualitative colorimetric detection of l-cysteine

“…MnO 2 nanosheet, a type of transition metal oxide, has low toxicity and good biocompatibility, rich redox chemistry, and excellent catalytic and oxidase-like activity. Owing to its superior chemical and physical properties, many researchers have combined MnO 2 nanosheets with chem/biosensing techniques for the determination of dopamine, 14 hydroquinone, 15 glutathione, 16 salmonella, 17 etc . More importantly, different from the reported peroxidase mimics for colorimetric sensing, MnO 2 nanosheets represent a label-free colorimetric probe that can oxidize the colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into a blue product (oxTMB), which has a characteristic UV–vis spectrum absorbance at 652 nm, without the addition of H 2 O 2 .…”