On-line multidimensional liquid chromatography and capillary electrophoresis systems for peptides and proteins

Theory and Practice of UHPLC and UHPLC–MS

“…Because of the tremendously higher separation power of LC Â LC when compared with its 1D-LC counterpart, the technique has been employed in several fields, e.g., polymers, pharmaceuticals and biological mixtures, natural products, environmental and petrochemical samples, and has been the subject of various reviews (Liu and Lee, 2000;Evans and Jorgenson, 2004;Guttman et al, 2004;Stroink et al, 2005;Dixon et al, 2006;Jandera, , 2012Shalliker and Gray, 2006;Shellie and Haddad, 2006;Dugo et al, 2008a,b;Pol and Hyotylainen, 2008;François et al, 2009a;Herrero et al, 2009;Sandra et al, 2009;Berek, 2010;Pierce et al 2012;Sarrut et al, 2014).…”

Comparative Studies Involving Transgenic and Non-Transgenic Soybean: What is Going On?

“…The second dimension must not decrease the resolution obtained using the previous one. For the separation mechanisms to be different, the columns must have different stationary phases, allowing the less efficient separation attained in the first dimension to be improved in the second [110].…”

Makings of the Best Spermatozoa: Molecular Determinants of High Fertility

“…This method reportedly reduces the between-gel variability and allows more precise comparisons between samples related to contrasting phenotypes or treatments. Chromatography is another method for protein separation, using matrices such as ion-exchange, gel-filtration, affinity and multidimensional (Stroink et al, 2005). Given the complexity of seminal plasma, preseparation of samples by chromatography enhances the detection of components during proteome-based studies.…”