On-line screening of matrix metalloproteinase inhibitors by capillary electrophoresis coupled to ESI mass spectrometry

Wang, Xu; Dou, Zhiying; Yuan, Yaozuo; Man, Shuli; Wolfs, Kris; Adams, Erwin; Schepdael, Ann Van

doi:10.1016/j.jchromb.2013.04.038

Cited by 22 publications

(17 citation statements)

References 33 publications

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“…The reaction products were pushed out of the capillary and off-line analysed by UHPLC TOF-MS. The real-time combination of EMMA with MS was first described by Van Schepdael et al who used this approach for an on-line screening of matrix metalloproteinase inhibitors by CE coupled to IT-MS [19]. Since the TDLFP methodology represents more versatile tool then EMMA, its possible combination with MS detection was conscientiously optimised in this study for such important enzymatic system such as CYPs.…”

Section: Resultsmentioning

confidence: 99%

“…The RSD of the repeatability of peak areas published by Wang et al who performed the in-capillary reaction of matrix metalloproteinase using the EMMA methodology combined with MS detection were better  3.2 % for classic and 4.8 % for pressure-mediated EMMA [19]. But it is necessary to point out that the CYP represents more complicated enzymatic system in contrast with the matrix metalloproteinase.…”

Section: Validation Of the Developed Methodsmentioning

confidence: 95%

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Combination of on‐line CE assay with MS detection for the study of drug metabolism by cytochromes P450

et al. 2015

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A new CE-MS method with enzymatic reaction inside the capillary was developed for the study of drug metabolism by cytochromes P450. This automated method, based on the transverse diffusion of laminar flow profiles methodology, is comprised of the injection of substrates and enzyme, their mixing, incubation, and separation of the reaction products, all performed by CE, and their detection, identification, and quantification by MS. The developed and validated method was finally used to conduct a kinetic study of cytochrome P450 isoform 2C9 or human liver microsomes with diclofenac in order to demonstrate its practical functionality. All the estimated kinetic values--apparent Michaelis constants and apparent maximum reaction velocities were in agreement with literature data obtained using other techniques. In addition, the consumption of reactants was in the tens of nL per analysis. The method's usability was further demonstrated on tolbutamide, the other probe substrate of cytochrome P450 isoform 2C9. As a result, the method is conceptually applicable for the screening of any other cytochrome P450 isoform and its substrates and inhibitors after adapting the incubation and separation conditions.

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Section: Resultsmentioning

confidence: 99%

Section: Validation Of the Developed Methodsmentioning

confidence: 95%

Combination of on‐line CE assay with MS detection for the study of drug metabolism by cytochromes P450

et al. 2015

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“… studied tyrosinase by using pressure mediated microanalysis (PMMA). PMMA is also an on‐line technique which mediates the enzyme reaction by pressure; both the mixing and separation proceed without voltage application by using only pressure. As there is no need for a resolving BGE solution, this technique is fast, convenient and simple.…”

Section: On‐line Enzyme Reaction Studiesmentioning

confidence: 99%

Advances in Capillary Electrophoretically Mediated Microanalysis for On‐line Enzymatic and Derivatization Reactions

et al. 2017

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This review summarizes recent developments, applications, and innovations of capillary electrophoretically mediated microanalysis methods. As a follow up of an earlier review, it covers the literature from early 2015 to early 2017. This article is divided into three parts. In the first part, different types of mixing procedures and applications of enzyme mediated microanalysis are discussed; the second part summarizes immobilized enzyme reactors (IMERs), while the third part deals with recent advances in on-line derivatization reactions.

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“…CE‐ESI‐MS using 20 mM ammonium acetate, pH 6.8, as BGE and water/MeOH, 1:1 v/v containing 0.5% formic acid as sheath liquid at a flow rate 4 μL/min has been applied to on‐line screening of matrix metalloproteinase inhibitors . The MS detection was operated in the positive ion mode with LOD 10 nM for the matrix metalloproteinase reaction peptide product (Mca‐Pro‐Leu‐Gly where Mca stands for (7‐methoxycoumarin‐4‐yl)acetyl) and provided 60‐fold enhancement of sensitivity by using selected reaction monitoring detection compared with full‐scan mode.…”

Section: Applicationsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2013–middle 2015)

Kašička

2015

Electrophoresis

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The review brings a comprehensive survey of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) to analysis, micropreparation, purification, and physicochemical and biochemical characterization of peptides in the years 2013, 2014, and ca. up to the middle of 2015. Advances in the investigation of electromigration properties of peptides, in the methodology of their analysis, including sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, as well as in detection of peptides, are described. New developments in particular CE and CEC modes are presented and several types of their applications to peptide analysis are reported: conventional qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence, and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC techniques to provide important physicochemical characteristics of peptides are demonstrated.

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On-line screening of matrix metalloproteinase inhibitors by capillary electrophoresis coupled to ESI mass spectrometry

Cited by 22 publications

References 33 publications

Combination of on‐line CE assay with MS detection for the study of drug metabolism by cytochromes P450

Combination of on‐line CE assay with MS detection for the study of drug metabolism by cytochromes P450

Advances in Capillary Electrophoretically Mediated Microanalysis for On‐line Enzymatic and Derivatization Reactions

Recent developments in capillary and microchip electroseparations of peptides (2013–middle 2015)

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