On-line TLC-MS

Prošek, Mirko; Milivojevic, Luka; Križman, Mitja; Fir, Maja

doi:10.1556/jpc.17.2004.6.4

Cited by 31 publications

(12 citation statements)

References 1 publication

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“…a sealing surface sampling-probe (SSSP) 119 and a liquid microjunction surface-sampling probe (LMJ-SSP). 120 While the emphasis in each case has been on coupling with ESI, the use of APCI has been demonstrated, 121,122 and there is no fundamental limitation to using other liquid introduction ionization sources such as APPI, ICP, or any other. Once in solution and carried into the source, the analyte ionization is governed by the processes fundamental to the particular ionization source.…”

Section: Liquid Extraction Surface-sampling Probe/ionizationmentioning

confidence: 99%

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Established and emerging atmospheric pressure surface sampling/ionization techniques for mass spectrometry

2008

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The number and type of atmospheric pressure techniques suitable for sampling analytes from surfaces, forming ions from these analytes, and subsequently transporting these ions into vacuum for interrogation by MS have rapidly expanded over the last several years. Moreover, the literature in this area is complicated by an explosion in acronyms for these techniques, many of which provide no information relating to the chemical or physical processes involved. In this tutorial article, we sort this vast array of techniques into relatively few categories on the basis of the approaches used for surface sampling and ionization. For each technique, we explain, as best known, many of the underlying principles of operation, describe representative applications, and in some cases, discuss needed research or advancements and attempt to forecast their future analytical utility.

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Section: Liquid Extraction Surface-sampling Probe/ionizationmentioning

confidence: 99%

“…119,121 However, the probe and elution concept by Luftmann 119 has been most often used because it is available commercially.…”

Section: Sealing Surface-sampling Probementioning

confidence: 99%

Established and emerging atmospheric pressure surface sampling/ionization techniques for mass spectrometry

2008

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“…22 The elution-head-based approach was demonstrated on TLC plates silica gel 60 coupled to MS via the Eluchrom automatic elution system (available from CAMAG, Muttenz, Switzerland until 1992), which eluted up to six zones in parallel ( Figure 2). 24 With specially constructed knives on the bottom of the elution head, a channel was cut around the zone of interest on the TLC silica gel plate. The scored plate was placed on the Eluchrom unit, and a Teflon elution head was exactly placed over the adsorbent-free glass circle.…”

Section: Adsorbent-free Seal (Eluchrom)mentioning

confidence: 99%

Planar Chromatography Mass Spectrometry

Morlock

2014

Reference Module in Chemistry, Molecular Sciences and Chemical Engineering

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“…[25] Various desorption-based techniques have been reported for coupling, e.g., fast atom bombardment (FAB), [26] secondary ion mass spectrometry (SIMS), [27] matrix assisted laser desorption=ionization (MALDI), [28][29][30] desorption electrospray ionization (DESI), [31] electrospray-assisted laser desorption=ionization mass spectrometry (ELDI-MS), [32] continuous wave diode laser desorption=atmospheric pressure chemical ionization (LD-APCI), [33] direct analysis in real time (DART), [34] easy ambient sonic-spray ionization mass spectrometry (EASI-MS), [35,36] and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). [37,38] Elution-based approaches include an automatic sampling surface probe coupled with electrospray ionization (ESI) by a micro-junction, [39] or elution-based interfaces coupled to APCI [40] or ESI. [41,42] The latter approach was successfully proven for qualitative [19,43,44] and quantitative analysis, [34,42] and, hence, it was used in this work.…”

Section: Introductionmentioning

confidence: 99%

Quantification of Pyritinol in Solid Pharmaceutical Formulation by High-Performance Thin-Layer Chromatography-Ultraviolet Detection and Selectivity Evaluation by Mass Spectrometry

Aranda

Morlock

2010

Journal of Liquid Chromatography & Related Technologies

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& A high-throughput method for pyritinol quantification in solid pharmaceutical formulation was developed. Chromatography was performed on silica gel 60 F 254 HPTLC plates using the mixture dichloromethane -methanol -formic acid 9:1:1 (v=v=v) as mobile phase. Detection was carried out by UV absorbance at 300 nm. Calibration showed a polynomial regression with a determination coefficient (R 2 ) of 0.9992. For chromatography, repeatability (relative standard deviation, RSD) and intermediate precision (RSD) in matrix were 0.4% and 3.0%, respectively. Recoveries of spiked samples at three levels ranging from 98.5 to 101.9% with intermediate precisions of RSD 3.7 to 4.7%. Limits of detection and quantification were 0.6 and 2.0 lg mL À1 (6 and 20 ng=band), respectively. The method capacity to detect degradation products and=or byproducts within routine conditions of analysis was evaluated through forced degradation processes. Selectivity was evaluated determining the peak purity by UV-spectrophotometry, which showed correlation coefficients (r) > 0.9997. Additionally, peak identity and purity was confirmed by mass spectrometry. The mass spectra showed just pyritinol ions at m=z 369 [M þH] þ and 391 [M þNa] þ being acquired directly from the sample bands by an elution-based interface. Considering the validation results, reduced analysis cost, accelerated analysis time, and high throughput capacity, this simple, yet reliable planar chromatographic method is a good alternative for pyritinol analysis in pharmaceutical formulations.

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On-line TLC-MS

Cited by 31 publications

References 1 publication

Established and emerging atmospheric pressure surface sampling/ionization techniques for mass spectrometry

Established and emerging atmospheric pressure surface sampling/ionization techniques for mass spectrometry

Planar Chromatography Mass Spectrometry

Quantification of Pyritinol in Solid Pharmaceutical Formulation by High-Performance Thin-Layer Chromatography-Ultraviolet Detection and Selectivity Evaluation by Mass Spectrometry

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