On-line two dimensional liquid chromatography/mass spectrometry for the analysis of triacylglycerides in peanut oil and mouse tissue

Yang, Qin; Shi, Xianzhe; Gu, Qun; Zhao, Sumin; Shan, Yuanhong; Xu, Guowang

doi:10.1016/j.jchromb.2012.03.013

Cited by 38 publications

(22 citation statements)

References 48 publications

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“…Various methods have been applied to the analysis of intact TAGs. Liquid chromatography‐mass spectrometry approaches (LC‐MS) have been utilized to analyze TAGs in sunflower and olive oils (Herrera et al ., ), yellow‐green algae (Rezanka et al ., ), microalgae (MacDougall et al ., ), pine seed (Acheampong et al ., ), peanut (Yang et al ., ; Hu et al ., ), and human chylomicron (Bonham et al ., ). Techniques based on matrix‐assisted laser desorption ionization‐time‐of‐flight MS have been applied to analysis of TAGs from olive oil (Chapagain and Wiesman, ), grape seeds (De Marchi et al ., ), and microalgae (Danielewicz et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Quantitative profiling and pattern analysis of triacylglycerol species in Arabidopsis seeds by electrospray ionization mass spectrometry

Baughman

Roth

et al. 2013

The Plant Journal

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SUMMARYPlant triacylglycerols (TAGs), or vegetable oils, provide approximately 25% of dietary calories to humans and are becoming an increasingly important source of renewable bioenergy and industrial feedstocks. TAGs are assembled by multiple enzymes in the endoplasmic reticulum from building blocks that include an invariable glycerol backbone and variable fatty acyl chains. It remains a challenge to elucidate the mechanism of synthesis of hundreds of different TAG species in planta. One reason is the lack of an efficient analytical approach quantifying individual molecular species. Here we report a rapid and quantitative TAG profiling approach for Arabidopsis seeds based on electrospray ionization tandem mass spectrometry with direct infusion and multiple neutral loss scans. The levels of 93 TAG molecular species, identified by their acyl components, were determined in Arabidopsis seeds. Quantitative TAG pattern analyses revealed that the TAG assembly machinery preferentially produces TAGs with one elongated fatty acid. The importance of the selectivity in oil synthesis was consistent with an observation that an Arabidopsis mutant overexpressing a patatin-like phospholipase had enhanced seed oil content with elongated fatty acids. This quantitative TAG profiling approach should facilitate investigations aimed at understanding the biochemical mechanisms of TAG metabolism in plants.

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Section: Introductionmentioning

confidence: 99%

Quantitative profiling and pattern analysis of triacylglycerol species in Arabidopsis seeds by electrospray ionization mass spectrometry

Baughman

Roth

et al. 2013

The Plant Journal

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“…The former may arise from inadequate peak focusing on the top of the 2 D column for a matter of solvent compatibility. In fact, the 1 D mobile phase, serving as the fraction solvent (hexane), possesses a higher eluent strength compared with the 2 D mobile phase, possibly compromising the whole LC × LC separation [26][27][28][29]. In this work, thanks to the transfer of 36 fraction volumes as low as 20 μl from 1 D to 2 D, it was possible to solve this issue, making the transfer of incompatible solvents possible without peak shape deterioration or resolution losses.…”

Section: Bstop-flow^ag + -Lc × Narp-lc Analyses Of the Triacylglyceromentioning

confidence: 99%

Determination of the triacylglycerol fraction in fish oil by comprehensive liquid chromatography techniques with the support of gas chromatography and mass spectrometry data

et al. 2015

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Fish oil made from menhaden (Brevoortia tyrannus) can be used as a dietary supplement for the presence of high levels of the long-chained omega-3 fatty acids, viz. epentaenoic and docosahexanoic. In this work, for the first time, two different multidimensional approaches were developed and compared, in terms of peak capacity, for triacylglycerol characterization. In particular, silver ion chromatography with a silver-ion column and non-aqueous reverse-phase liquid chromatography with a C18 column were tested in both comprehensive (stop-flow) and off-line modes. The use of mass spectra attained by atmospheric pressure chemical ionization for both LC approaches, and the fatty acids methyl esters profile of menhaden oil obtained by gas chromatography analysis, greatly supported the elucidation of the triacylglycerol content in menhaden oil. The off-line approach afforded a better separation and, thus, higher peak capacity to allow identifying and semiquantifying more than 250 triacylglycerols. Such a huge number has never been reported for a menhaden oil sample.The main disadvantage of such an approach over the stop-flow one was the longer analysis time, mainly attributable to solvent exchange between the two dimensions.

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“…The theory of 2DLC was first proposed by Schoenmakers et al (2003) and has been successfully applied to separate multiple compounds, such as phenolic compounds (Montero et al 2013), tannins (Kalili et al 2013), saponins (Wang et al 2008), triacylglycerides (Yang et al 2012) and phospholipids (Dugo et al 2013). This method has also been used for proteomics and metabolomics analyses (Shi et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Acylated Anthocyanins in Red Cabbage via Comprehensive Two-Dimensional High Performance Liquid Chromatography and HPLC-MS

Zhang

Wang

2016

Journal of Food Processing and Preservation

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Red cabbage (Brassica oleracea L. Var. Capiata L.) is a fresh edible vegetable abundant in acylated anthocyanins with high antioxidant properties and is a promising resource for the functional food industry. Anthocyanin characterization of red cabbage is not a new topic, but the predominant anthocyanins have been co‐eluted using high performance liquid chromatography (HPLC) because of their similar polarities, and it was difficult to achieve a baseline separation of anthocyanins in red cabbage. In the present paper, we optimized the separation of anthocyanins using a two‐dimensional HPLC system (2DLC). Using a selective separation column, adjusting the switching time, and optimizing the elution program, seven anthocyanins were separated quickly with a good resolution, and identified via HPLC‐mass spectrometry. Therefore, the 2DLC technique proved to be a powerful method of qualitative and quantitative analysis for the anthocyanins of red cabbage. Additionally, this method provides a good reference for the separation of acylated anthocyanins in fruits and vegetables. Practical Applications Red cabbage (Brassica oleracea L. Var. Capiata L.) is a fresh edible vegetable abundant in acylated anthocyanins with high antioxidant properties, and is a promising resource for the functional food industry. This paper describes the development and evaluation of a two‐dimensional HPLC system (2DLC) method for the separation of acylated anthocyanins from red cabbage, which provides a good reference for the separation of acylated anthocyanins in fruits and vegetables. And there are many thousands of scientists whose work involve flavonoids, and our work offers a clear example of how new analytical approaches can be employed to increase analytical resolution and therefore enable researchers to clarify previously ambiguous or confounding results. We believe our findings are of interest to readers in food analysis, post‐harvest and storage physiology, and to plant biologists that are trying to solve biochemical questions that previous analytical methods have failed to address adequately.

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On-line two dimensional liquid chromatography/mass spectrometry for the analysis of triacylglycerides in peanut oil and mouse tissue

Cited by 38 publications

References 48 publications

Quantitative profiling and pattern analysis of triacylglycerol species in Arabidopsis seeds by electrospray ionization mass spectrometry

Quantitative profiling and pattern analysis of triacylglycerol species in Arabidopsis seeds by electrospray ionization mass spectrometry

Determination of the triacylglycerol fraction in fish oil by comprehensive liquid chromatography techniques with the support of gas chromatography and mass spectrometry data

Characterization of Acylated Anthocyanins in Red Cabbage via Comprehensive Two-Dimensional High Performance Liquid Chromatography and HPLC-MS

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