On selection of methods and media for isolation of freshwater actinomycetes

Jayashree, S.; Vyas, Vipin; Bhatnagar, G.P.; Syed, Salman Ali

doi:10.1080/09593339109385119

Cited by 7 publications

(4 citation statements)

References 7 publications

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“…However, Actinomycetes Isolation Agar (AIA) and Soybean Casein Digest Agar (SCDA) were found to be most suitable, but with enhanced incubation period and incubation temperature (37–39°C instead of 28–30°C). For isolation of actinomycetes, AIA[ 11 ] and for other Eubacterial isolation SCDA[ 12 13 ] were used. Amphotercine B was incorporated in both media at 20μg/ml concentration to limit the fungal growth, which often overgrow the isolation plates.…”

Section: Methodsmentioning

confidence: 99%

Anti-infective potential of hot-spring bacteria

Pednekar

Jain

Mahajan

2011

J Global Infect Dis

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Aim and Background:Antibiotic resistance currently spans most of the known classes of natural and synthetic antibiotics; limiting our options for treatment of infections and demanding discovery of new classes of antibiotics. Much effort is being directed towards developing new antibiotics to overcome this problem. Success in getting novel chemical entities from microbial sources depends essentially on novelty of its habitat. The diversity of geographical location decides the type of micro-flora. In the past various terrestrial and aqueous microorganisms have provided several novel bioactive secondary metabolites of pharmaceutical importance. Hot-springs have not been as extensively exploited as other terrestrial resources. However, perseverance with such microbes augment the probability of getting novel bioactive compounds.Materials and Methods:Hot-springs soil samples were collected from Hot-springs in Maharashtra. Actinomycetes and other eubacteria were isolated from these soil samples by selective methods and purified. They were classified based on gram's nature and morphology. Six representative morphological strains were screened for their anti-infective potential by agar well diffusion method as reported by Nathan P. et al (1974). The bioactivity of the active microbes was confirmed.Results:Seventy three strains of bacteria encompassing eight actinomycetes, and 65 eubacteria were isolated and purified. Among the actives eubacteria PPVWK106001 showed broad spectrum antibacterial activity encompassing both gram positive and gram negative bacterial test models. The extract was active against resistant bacteria such as MRSA and VREs. Activity was very specific as there was no activity against fungi even at 100 fold concentration. The active principle was extractable in butanol.Conclusions:The study showed that Hot-springs exhibit diverse bacteria and it serves as potential reservoirs for bacteria of antimicrobial importance with diverse facet of activities. Thus Hot-springs microbes have ability to address issue of resistant bugs.

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Section: Methodsmentioning

confidence: 99%

Anti-infective potential of hot-spring bacteria

Pednekar

Jain

Mahajan

2011

J Global Infect Dis

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“…wet heat (50°C, 15 min) as physical treatments and T3. 0.05% SDS and 6% yeast extract for 30 min as a chemical treatment (Jayashree et al, 1991). Serial dilutions of the pretreated samples were prepared with a 2% (w/v) sodium chloride solution down to 10 − 4 .…”

Section: Sample Collection Media Cultures and Strain Isolationmentioning

confidence: 99%

Bioactive compounds of the Actinomycetes isolated from Garmsar Saline River, Iran

Salehghamari,

Sardabi,

Bahrami

et al. 2024

Preprint

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Saline environments are unique ecosystems that harbor novel bacterial species with the potential to produce valuable secondary metabolites. In the present study, 26 actinomycetes strains were recovered predominantly from the rhizosphere (81%), in addition to sediments and water samples collected from four sites along the Garmsar Saline River in Iran. According to phylogenetic analysis, the isolated strains were identified as belonging to two different genera: Streptomyces, and Nocardiopsis. Several of the isolated actinomycetes exhibited valuable biotechnological potential, producing a diversity of enzymes including amylase, gelatinase, protease, lipase, lecithinase, and laccase. Inhibitory effects on the growth of human tumor cells were also observed; the extract of strain M15 displayed notable cytotoxicity against MCF7 human breast cancer cells with an IC50 value of 32.81 µg/ml. Also, 59% of the strains had antibacterial activity against some pathogenic and non-pathogenic test strains. Ethyl acetate extracts of the selected actinomycetes were screened, and strain M16 was found to exhibit the most potent inhibitory effect on Proteus mirabilis. GC/MS analysis of M16’s extract revealed it mainly consisted of 1,2-benzenedicarboxylic acid, bis(2-methylpropyl) ester (16.61%), and 9-octadecenamide (12.77%). Based on 16S rRNA sequence analysis, Strain M16 showed the highest similarity to Nocardia aegyptia. The study concludes that actinomycetes from the Garmsar Saline River possess considerable potential for biotechnological application.

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“…0.05 % SDS and 6 % yeast extract for 30 min and T4. wet heat(50°C, 15 min) (Jayashree et al 1991). The pretreated samples were diluted (1:10 v/v) with a 5% saline solution and mixed for one hour.…”

Section: Sample Pretreatments and The Bacterial Growth Conditionsmentioning

confidence: 99%

Actinomycetes of Howz Soltan Lake and their metabolite diversity in Iran

Salehghamari

Moradi

Sardabi

et al. 2022

Preprint

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Saline environments are largely unexplored sources for actinomycetes with the potential to produce biologically active secondary metabolites. A total of 34 actinomycete strains from water, sediments and mostly rhizosphere (82%) were collected from different sites of Howz Soltan Lake in Iran. Based on phylogenetic analysis, the isolated strains belonged to the genera Streptomyces, Nocardia and Saccharomonospora. Among the isolated actinomycetes, several strains had valuable biotechnological applications such as growth inhibition of human tumor cells. The results of cytotoxic assay revealed strain act9 having the most potent extract (19.716 ± 5.72 µg/ml) against the MDA-MB-231 human breast cancer cell. Also 38% of the strains had an antimicrobial activity against some pathogenic and nonpathogenic microorganisms. The ethyl-acetate extract of act18 showed the most antibacterial effect against Staphylococcus aureus, MRSA, and was further analyzed by GC/MS. Ar-tumerone (26.41%) and Butyl isodecyl phthalate (21.77%) were the main constituents detected in the extract.. This is the first time this compound is being detected in a prokaryote. Act18 showed the most 16s rRNA sequence similarity to Streptomyces youssoufiensis. In addition, a number of the isolates produced different enzymes including lipase, amylase, protease, gelatinase, urease and lecetinase. Also, the plant growth promoting activity of some actinomycete strains were shown. Some of the strains belonging to the genera Streptomyces and Nocardia increased the seed germination, stem length and the number of Echium leaves during the 20 days. Consequently, it was concluded that actinomycetes isolated from these saline lake habitats are potentially valuable for biotechnological applications.

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On selection of methods and media for isolation of freshwater actinomycetes

Cited by 7 publications

References 7 publications

Anti-infective potential of hot-spring bacteria

Anti-infective potential of hot-spring bacteria

Bioactive compounds of the Actinomycetes isolated from Garmsar Saline River, Iran

Actinomycetes of Howz Soltan Lake and their metabolite diversity in Iran

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