2018
DOI: 10.3791/56891
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On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

Abstract: On-site diagnosis of plant diseases can be a useful tool for growers for timely decisions enabling the earlier implementation of disease management strategies that reduce the impact of the disease. Presently in many diagnostic laboratories, the polymerase chain reaction (PCR), particularly real-time PCR, is considered the most sensitive and accurate method for plant pathogen detection. However, laboratory-based PCRs typically require expensive laboratory equipment and skilled personnel. In this study, soil-bor… Show more

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Cited by 17 publications
(10 citation statements)
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“…We recommend development of diagnostic molecular markers of P. belbahrii sporangia to enable field based pathogen detection. Portable PCR equipment has been utilized to detect fungal and viral pathogens on important plant commodities (Babu et al., 2017; DeShields et al., 2018).…”
Section: Discussionmentioning
confidence: 99%
“…We recommend development of diagnostic molecular markers of P. belbahrii sporangia to enable field based pathogen detection. Portable PCR equipment has been utilized to detect fungal and viral pathogens on important plant commodities (Babu et al., 2017; DeShields et al., 2018).…”
Section: Discussionmentioning
confidence: 99%
“…In addition to the aforementioned works, several other efforts related to PCR-based phytopathogen detection have been reported in the past decade, such as a multiplex RT-PCR (mRT-PCR) method for simultaneous detection of five grapevine viroids [59], the use of qPCR for the detection and quantification of filamentous fungi and oomycetes within host tissue, soil, air, and water [60], a qPCR assay for the detection and quantification of Verticillium dahliae in spinach seed resulting in reliable measurements with sensitivity limit of about 1 infected seed per 100 [61], and an on-site system for detecting soil-borne pathogen (Spongospora subterranea) that causes Powdery scab in potato using magnetic bead-based nucleic acid extraction, and portable qPCR (fluorogenic probe-based assay) [62].…”
Section: Pcr-based Methodsmentioning
confidence: 99%
“…Quick and reliable diagnostic tools for specific detection of Fusarium oxysporum gladioli infection in propagation material can be explored by vegetative compatibility grouping [116]. With the advancement in DNA-based diagnostic tests which have numerous advantages, such as non-culturing of the target pathogens, ability to identify and detect an individual strain from the mixed sample, quantification of the target pathogen, detection of multiple pathogens, less time consuming and cost effective, help in the identification and enumeration of the pathogenic strains of corm rot of saffron [117].…”
Section: Exclusionmentioning
confidence: 99%