On the antibacterial mechanism of graphene oxide (GO) Langmuir–Blodgett films

Mangadlao, Joey Dacula; Santos, Cid Aimbiré de Moraes; Felipe, Mary Jane; Leon, Alex; Rodrigues, Débora F.; Advíncula, Rigoberto C.

doi:10.1039/c4cc07836e

Cited by 244 publications

(195 citation statements)

References 32 publications

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“…Indeed, several graphene-derived substrates with oxygen-containing functional groups may produce a surplus of ROS, which can contribute to the antimicrobial effect, but it could be also correlated to a higher cytotoxicity [44,45]. …”

Section: Resultsmentioning

confidence: 99%

Zinc Oxide Nanorods-Decorated Graphene Nanoplatelets: A Promising Antimicrobial Agent against the Cariogenic Bacterium Streptococcus mutans

et al. 2016

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Nanomaterials are revolutionizing the field of medicine to improve the quality of life due to the myriad of applications stemming from their unique properties, including the antimicrobial activity against pathogens. In this study, the antimicrobial and antibiofilm properties of a novel nanomaterial composed by zinc oxide nanorods-decorated graphene nanoplatelets (ZNGs) are investigated. ZNGs were produced by hydrothermal method and characterized through field-emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX) and X-ray diffraction (XRD) techniques. The antimicrobial activity of ZNGs was evaluated against Streptococcus mutans, the main bacteriological agent in the etiology of dental caries. Cell viability assay demonstrated that ZNGs exerted a strikingly high killing effect on S. mutans cells in a dose-dependent manner. Moreover, FE-SEM analysis revealed relevant mechanical damages exerted by ZNGs at the cell surface of this dental pathogen rather than reactive oxygen species (ROS) generation. In addition, inductively coupled plasma mass spectrometry (ICP-MS) measurements showed negligible zinc dissolution, demonstrating that zinc ion release in the suspension is not associated with the high cell mortality rate. Finally, our data indicated that also S. mutans biofilm formation was affected by the presence of graphene-zinc oxide (ZnO) based material, as witnessed by the safranin staining and growth curve analysis. Therefore, ZNGs can be a remarkable nanobactericide against one of the main dental pathogens. The potential applications in dental care and therapy are very promising.

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Section: Resultsmentioning

confidence: 99%

Zinc Oxide Nanorods-Decorated Graphene Nanoplatelets: A Promising Antimicrobial Agent against the Cariogenic Bacterium Streptococcus mutans

et al. 2016

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“…In other studies, anti-microbial activity of graphene oxide (GO) has been attributed to induction of microbial membrane damage, disturbance of the membrane potential [93] and electron transport [94], as well as oxidative stress by increased production of reactive oxygen species (ROS) [95,96]. Antibacterial properties of graphene oxide were also found to be dependent on the size of the GO sheets: larger GO sheets, wrapping bacterial cells, can effectively isolate bacteria from their environment, and show stronger antibacterial activity as compared to small GO sheets [97].…”

Section: Antifungal and Antibacterial Agentsmentioning

confidence: 99%

Carbon nanomaterials: production, impact on plant development, agricultural and environmental applications

Zaytseva

Neumann

2016

Chem. Biol. Technol. Agric.

404

166

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During the relatively short time since the discovery of fullerenes in 1985, carbon nanotubes in 1991, and graphene in 2004, the unique properties of carbon-based nanomaterials have attracted great interest, which has promoted the development of methods for large-scale industrial production. The continuously increasing commercial use of engineered carbon-based nanomaterials includes technical, medical, environmental and agricultural applications. Regardless of the application field, this is also associated with an increasing trend of intentional or unintended release of carbon nanomaterials into the environment, where the effect on living organisms is still difficult to predict. This review describes the different types of carbon-based nanomaterials, major production techniques and important trends for agricultural and environmental applications. The current status of research regarding the impact of carbon nanomaterials on plant growth and development is summarized, also addressing the currently most relevant knowledge gaps.

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“…[21][22][23] For instance, graphene (and graphene oxide) has been systematically studied 24 for its antimicrobial activities, [25][26][27][28] along with its underlying molecular mechanisms. [29][30][31][32] It was revealed that graphene and graphene oxide nanosheets can cut into cell membranes, 32 extract large amount of phospholipids, 32 and create holes (pores) on cell membranes, 33 through large scale molecular dynamics simulations.…”

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confidence: 99%

Membrane destruction-mediated antibacterial activity of tungsten disulfide (WS₂)

et al. 2017

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The antibacterial activities of tungsten disulfide (WS 2 ) nanosheets against two representative bacterial strains: Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus)were evaluated by colony-forming unit (CFU) studies. The WS 2 samples demonstrate a time and concentration dependent antibacterial activity (retardation of bacterial growth) for both bacterial strains.Morphology analyses reveal that WS 2 nanosheets adhere to the bacterial surfaces, resulting in robust inhibition of cell proliferation once a bacterium is fully covered with this nanomaterial. More importantly, the intimate contact of WS 2 nanosheets with a bacterium cell membrane can cause serious damage to the membrane integrity, and subsequently the cell death. On the other hand, the reactive oxygen species (ROS) generated by WS 2 nanosheets are found to be modest regardless of the WS 2 concentration, which is contradictory to the case of its structural analogue, MoS 2 , where ROS also play a significant role in its antibacterial activity. Taken together, our findings provide a detailed understanding of the antibacterial mechanism of WS 2 nanosheets, which might help promote their potential applications in biomedical fields.

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On the antibacterial mechanism of graphene oxide (GO) Langmuir–Blodgett films

Cited by 244 publications

References 32 publications

Zinc Oxide Nanorods-Decorated Graphene Nanoplatelets: A Promising Antimicrobial Agent against the Cariogenic Bacterium Streptococcus mutans

Zinc Oxide Nanorods-Decorated Graphene Nanoplatelets: A Promising Antimicrobial Agent against the Cariogenic Bacterium Streptococcus mutans

Carbon nanomaterials: production, impact on plant development, agricultural and environmental applications

Membrane destruction-mediated antibacterial activity of tungsten disulfide (WS₂)

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On the antibacterial mechanism of graphene oxide (GO) Langmuir–Blodgett films

Cited by 244 publications

References 32 publications

Zinc Oxide Nanorods-Decorated Graphene Nanoplatelets: A Promising Antimicrobial Agent against the Cariogenic Bacterium Streptococcus mutans

Zinc Oxide Nanorods-Decorated Graphene Nanoplatelets: A Promising Antimicrobial Agent against the Cariogenic Bacterium Streptococcus mutans

Carbon nanomaterials: production, impact on plant development, agricultural and environmental applications

Membrane destruction-mediated antibacterial activity of tungsten disulfide (WS2)

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Membrane destruction-mediated antibacterial activity of tungsten disulfide (WS₂)