On the Anticataractogenic Effects of L‐Carnosine: Is It Best Described as an Antioxidant, Metal‐Chelating Agent or Glycation Inhibitor?

Abdelkader, Hamdy; Longman, Michael R.; Alany, Raid G.; Pierscionek, Barbara K.

doi:10.1155/2016/3240261

Cited by 28 publications

(21 citation statements)

References 36 publications

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“…The ability of EGCNPs to prevent protein glycation was determined by measuring the distinctive fluorescence of advanced glycation end products (AGEs) as previously reported [24]. Briefly, two solutions of bovine serum albumin (BSA, 10 mg/mL) and Dglucose (10 mg/mL) were separately made in PBS (pH = 7).…”

Section: Measuring Antiglycation Properties Of Egcnps On Bsa and Bovine α-Crystallinmentioning

confidence: 99%

Nanoceria Prevents Glucose-Induced Protein Glycation in Eye Lens Cells

et al. 2021

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Cerium oxide nanoparticles (nanoceria) are generally known for their recyclable antioxidative properties making them an appealing biomaterial for protecting against physiological and pathological age-related changes that are caused by reactive oxygen species (ROS). Cataract is one such pathology that has been associated with oxidation and glycation of the lens proteins (crystallins) leading to aggregation and opacification. A novel coated nanoceria formulation has been previously shown to enter the human lens epithelial cells (HLECs) and protect them from oxidative stress induced by hydrogen peroxide (H2O2). In this work, the mechanism of nanoceria uptake in HLECs is studied and multiple anti-cataractogenic properties are assessed in vitro. Our results show that the nanoceria provide multiple beneficial actions to delay cataract progression by (1) acting as a catalase mimetic in cells with inhibited catalase, (2) improving reduced to oxidised glutathione ratio (GSH/GSSG) in HLECs, and (3) inhibiting the non-enzymatic glucose-induced glycation of the chaperone lens protein α-crystallin. Given the multifactorial nature of cataract progression, the varied actions of nanoceria render them promising candidates for potential non-surgical therapeutic treatment.

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Section: Measuring Antiglycation Properties Of Egcnps On Bsa and Bovine α-Crystallinmentioning

confidence: 99%

Nanoceria Prevents Glucose-Induced Protein Glycation in Eye Lens Cells

et al. 2021

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“…These glycation reactions serve as the initial step in the development of diabetic pathologies (Hipkiss et al, 1995). In a study by Abdelkader et al (2016), carnosine was shown to have effective anti-glycation activity in cultured porcine lenses and human lens epithelial cell models. Even in the absence of a diabetic condition, the use of carnosine as a supplement may also help reduce glycosylated damage on lens redox glutathione system.…”

Section: Heath Benefitsmentioning

confidence: 99%

Carnosine—a natural bioactive dipeptide: bioaccessibility, bioavailability and health benefits

Xing¹,

Chee²,

Zhang³

et al. 2019

Journal of Food Bioactives

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Carnosine is a natural dipeptide synthesized by both vertebrate and invertebrate organisms and has functional properties that are specific to muscle and excitable tissues. Recent in vivo and in vitro studies have shown that carnosine presents metal chelating and antioxidant activities and has the ability to inhibit protein carbonylation and glycoxidation. This review describes the health benefits of carnosine in relation to its bioaccessibility, bioavailability and biochemical properties as well as providing the current state-of-the-art knowledge on the potential use of carnosine as a nutraceutical. The therapeutic potential of carnosine has also been investigated by a number of preclinical and clinical studies for diseases such as diabetes and its associated complications, as well as fatigue, ageing, and some neurological disorders. Altogether the current literature provides supportive evidence on the use of carnosine as a natural dietary supplement with significant health boosting efficiency and without any side effects.

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“…Carnosine was selected due to its established antioxidant, free radical scavenging, antiinflammatory and wound-healing properties [12][13][14]. This dipeptide is widely distributed in muscles and nervous tissues, where it plays an important protective function [15].…”

Section: Introductionmentioning

confidence: 99%

Lipidation of Temporin-1CEb Derivatives as a Tool for Activity Improvement, Pros and Cons of the Approach

Kosikowska-Adamus

Sikorska

Wyrzykowski

et al. 2021

IJMS

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The alarming raise of multi-drug resistance among human microbial pathogens makes the development of novel therapeutics a priority task. In contrast to conventional antibiotics, antimicrobial peptides (AMPs), besides evoking a broad spectrum of activity against microorganisms, could offer additional benefits, such as the ability to neutralize toxins, modulate inflammatory response, eradicate bacterial and fungal biofilms or prevent their development. The latter properties are of special interest, as most antibiotics available on the market have limited ability to diffuse through rigid structures of biofilms. Lipidation of AMPs is considered as an effective approach for enhancement of their antimicrobial potential and in vivo stability; however, it could also have undesired impact on selectivity, solubility or the aggregation state of the modified peptides. In the present work, we describe the results of structural modifications of compounds designed based on cationic antimicrobial peptides DK5 and CAR-PEG-DK5, derivatized at their N-terminal part with fatty acids with different lengths of carbon chain. The proposed modifications substantially improved antimicrobial properties of the final compounds and their effectiveness in inhibition of biofilm development as well as eradication of pre-formed 24 h old biofilms of Candida albicans and Staphylococcus aureus. The most active compounds (C5-DK5, C12-DK5 and C12-CAR-PEG-DK5) were also potent against multi-drug resistant Staphylococcus aureus USA300 strain and clinical isolates of Pseudomonas aeruginosa. Both experimental and in silico methods revealed strong correlation between the length of fatty acid attached to the peptides and their final membranolytic properties, tendency to self-assemble and cytotoxicity.

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On the Anticataractogenic Effects of L‐Carnosine: Is It Best Described as an Antioxidant, Metal‐Chelating Agent or Glycation Inhibitor?

Cited by 28 publications

References 36 publications

Nanoceria Prevents Glucose-Induced Protein Glycation in Eye Lens Cells

Nanoceria Prevents Glucose-Induced Protein Glycation in Eye Lens Cells

Carnosine—a natural bioactive dipeptide: bioaccessibility, bioavailability and health benefits

Lipidation of Temporin-1CEb Derivatives as a Tool for Activity Improvement, Pros and Cons of the Approach

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