On the Convenient Method for Glucosamine Estimation in Koji

Sakurai, Yonekichi; Lee, Tae Ho; Shiota, Hideo

doi:10.1080/00021369.1977.10862552

Cited by 48 publications

(35 citation statements)

References 2 publications

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“…Glycoamylase units (IU) are expressed as ~01 reducing sugars released per min by the total amount of enzyme extracted from 1 g dry substrate (g ds). Mycelial growth was determined by estimating glucosamine in the fermenting substrate (Sakurai et al 1977). The substrate aW was measured using a water activity meter.…”

Section: Methodsmentioning

confidence: 99%

Influence of water activity on growth and activity of Aspergillus niger for glycoamylase production in solid-state fermentation

Pandey¹,

Lakshmikuttyamma²,

Selvakumar³

et al. 1994

World J Microbiol Biotechnol

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Section: Methodsmentioning

confidence: 99%

Influence of water activity on growth and activity of Aspergillus niger for glycoamylase production in solid-state fermentation

Pandey¹,

Lakshmikuttyamma²,

Selvakumar³

et al. 1994

World J Microbiol Biotechnol

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“…Biomass was estimated by determining the N-acetyl glucosamine released by the acid hydrolysis of chitin, present in the cell wall of the fungi [26]. Then 0.5 g of fermented substrate previously dried at 110°C for 2 h in a hot-air oven was subjected to acid hydrolysis with 1 ml of concentrated sulphuric acid.…”

Section: Biomass Estimationmentioning

confidence: 99%

Studies on the production of nigerloxin using agro-industrial residues by solid-state fermentation

Chakradhar

Javeed

Sattur

2009

J Ind Microbiol Biotechnol

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Nigerloxin, a new and potent lipoxygenase inhibitor, was discovered in our laboratory through solid-state fermentation of wheat bran by Aspergillus niger V. Teigh (MTCC-5166). The aim of this study is to investigate the possibility of using different agro-industrial residues as nutritional supplements along with wheat bran to enhance the production of nigerloxin. Nigerloxin produced by SSF was quantified spectrophotometrically at 292 nm. The results indicate that the inhibitor production was influenced by the type of solid substrate supplemented, moisture content, pH and size of the inoculum. Individually optimized supplements were tested in different combinations to determine their effects on nigerloxin production. A twofold increase in the production of nigerloxin (4.9 +/- 0.3 mg gds(-1)) was achieved by supplementing wheat bran with 10% w/w sweet lemon peel and 5% v/w methanol at optimized process parameters, that is, an initial moisture content of 65% v/w and incubation period of 6 days with an initial inoculum size of 2 ml (8 x 10(5) spores gds(-1)). Nigerloxin production was stable between pH of 4 and 5.

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“…Biomass estimation was carried out by determining the Nacetyl glucosamine released by the acid hydrolysis of chitin present in the cell wall [30]. It was expressed as mg of glucosamine/g dry fermented matter.…”

Section: Biomass Estimationmentioning

confidence: 99%

Utilization of rice straw for laccase production by Streptomyces psammoticus in solid-state fermentation

Niladevi

Sukumaran

Prema

2007

J Ind Microbiol Biotechnol

103

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Laccase production from a novel actinobacterial strain, Streptomyces psammoticus, MTCC 7334 was optimized in solid-state fermentation. The process parameters were initially optimized by the conventional "one factor at a time" approach, and the optimal levels of the factors that had considerable influence on enzyme production were identified by response surface methodology. Rice straw was identified as a suitable substrate for laccase production (17.3 U/g), followed by coffee pulp (15.8 U/g). Other optimized conditions were particle size, 500-1,000 mum (21.2 U/g); initial moisture content, 65% (26.8 U/g); pH of moistening solution, 8.0 (26.9 U/g); incubation temperature, 32 degrees C (27.6 U/g) and inoculum size, 1.5 x 10(7) CFU (33.8 U/g). Yeast extract served as the best nitrogen source (34.8 U/g). No enhancement in enzyme yield was observed with carbon supplementation. The level of yeast extract, inoculum size and copper sulphate were optimized statistically. Statistical optimization performed using a central composite design resulted in threefold increase in laccase activity (55.4 U/g) as compared to the unoptimized medium (17.3 U/g). The upgrading of fermented rice straw for fodder enhancement is also discussed briefly.

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On the Convenient Method for Glucosamine Estimation in Koji

Cited by 48 publications

References 2 publications

Influence of water activity on growth and activity of Aspergillus niger for glycoamylase production in solid-state fermentation

Influence of water activity on growth and activity of Aspergillus niger for glycoamylase production in solid-state fermentation

Studies on the production of nigerloxin using agro-industrial residues by solid-state fermentation

Utilization of rice straw for laccase production by Streptomyces psammoticus in solid-state fermentation

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