On the Crucial Cerebellar Wound Healing-Related Pathways and Their Cross-Talks after Traumatic Brain Injury in Danio rerio

Abstract

Upon injury, the direct damage and the subsequent secondary injury in the brain often result in chronic neurological disorders. Due to multifactorial nature of secondary injury and subsequent complex cellular responses, much of the underlying mechanisms are unclear. This study used an adult zebrafish cerebellum injury model to investigate the phenotypes and the secondary injury responses for recovery mechanisms of injured brain. Using the time course microarray analysis, a candidate protein-protein interaction (PPI) network was refined as cerebellar wound healing PPI network by dynamic modeling and big data mining. Pathway enrichment and ontological analysis were incorporated into the refined network to highlight the main molecular scheme of cerebellar wound healing. Several significant pathways, including chemokine, Phosphatidylinositide 3-kinases, and axon guidance signaling pathway and their cross-talks through PI3K, PAK2, and PLXNA3 were identified to coordinate for neurogenesis and angiogenesis, which are essential for the restoration of the injured brain. Our finding provides an insight into the molecular restoration mechanisms after traumatic brain injury, and open up new opportunity to devise the treatment for traumatic brain injury in human.

Identification of network-based biomarkers of cardioembolic stroke using a systems biology approach with time series data

“…We compared the results with our previous study, "On the Crucial Cerebellar Wound Healing-Related Pathways and Their Cross-Talks after Traumatic Brain Injury in Danio rerio [ 35 ]". We found that there were no intersections between these 2 results.…”

Substrate-mediated reprogramming of human fibroblasts into neural crest stem-like cells and their applications in neural repair

“…The first group was adult zebrafish without lesion as a blank control. The other groups were injured by syringe (27G; Thermo Scientific, USA) to cause lesion in cerebellum [28]. The treatments on adult zebrafish were transplanted with FOXD3-transfected cells or nontransfected cells encapsulated in the chitosan-based self-healing hydrogel.…”

A Systems Biology Approach to the Coordination of Defensive and Offensive Molecular Mechanisms in the Innate and Adaptive Host–Pathogen Interaction Networks

“…Specifically, dynamic host–pathogen protein–protein interaction networks (HP-PPINs) can be used to bridge the gap between infection-activated molecular mechanisms and physiological phenomena. Moreover, dynamic PPINs quantitatively delineate the effects of current protein levels on the expression of other proteins [ 7 ] and can, therefore, be used to characterize the molecular mechanisms behind the interactions of host and pathogen proteins during the infection process. Hence, relating the infection-activated molecular mechanisms to physiological phenomena using dynamic HP-PPINs may inform biomedical applications from the investigations of HPIs.…”