On the deposition of phospholipids onto planar supports with the Langmuir—Blodgett technique using factorial experimental design 1. Screening of various factors and supports

Sellström, Åke; Gustafson, Inga; Ohlsson, Per-Åke; Olofsson, G.; Puu, Gertrud

doi:10.1016/0166-6622(92)80107-d

Cited by 13 publications

(5 citation statements)

References 21 publications

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“…The substrata were cleaned as described earlier it has been observed that phospholipids with choline endgroups could not be deposited as consecutive layers. − As we observed a similar behavior using different subphases and deposition conditions, we switched to phosphatidic acids. − …”

Section: Methodsmentioning

confidence: 82%

“…[34][35][36][37] As we observed a similar behavior using different subphases and deposition conditions, we switched to phosphatidic acids. [38][39][40][41][42][43][44][45] For the deposition of DOPA, a subphase of Milli-Q water with 5 × 10 -4 M CdCl 2 at pH 5.7 at room temperature was used. Monolayers of DOPA mixed with various amounts of RB18 were, after spreading on the water surface, compressed at a rate of 10 mm min -1 until a surface pressure of 30 mN m -1 was reached.…”

Section: Methodsmentioning

confidence: 99%

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Global Analysis of the Fluorescence Decays of N,N‘-Dioctadecyl Rhodamine B in Langmuir−Blodgett Films of Diacylphosphatidic Acids

Ballet¹,

Auweraer²,

Schryver³

et al. 1996

J. Phys. Chem.

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Langmuir-Blodgett multilayer films were prepared from N,N′-dioctadecyl rhodamine B (RB18) diluted in dioleoylphosphatidic acid (DOPA) or dipalmitoylphosphatidic acid (DPPA) with various mixing ratios. Surface pressure-area isotherms of monolayers of the two matrices were recorded. Absorption and fluorescence spectra and fluorescence decays of the multilayer samples of RB18 were further examined. The fluorescence decay curves of RB18 in the multilayer assemblies, determined using time-correlated single photon counting, were analyzed in the framework of several models leading to nonexponential decays: (1) multiexponential decay; (2) Gaussian distribution of rate constants; (3) stretched-exponential decay, characteristic of Förster energy transfer in self-similar fractal-like structures with a fractal dimensionality of <2; and (4) Förster energy transfer in a two-dimensional system accompanied by an unquenched decay. Using the global analysis method, only the decay law for Förster energy transfer in two dimensions accompanied by a monoexponential decay yielded acceptable statistical fits for multilayer assemblies of RB18 and DOPA or DPPA in the concentration range from 0.2 to 5 mol %. While global analysis of nonexponential decay profiles gave adequate parameters over the whole concentration range studied, single-curve analysis yielded less accurate values for the decay parameters, which became physically unacceptable at the highest and lowest dye concentrations studied. For the DOPA multilayer systems the recovered decay parameters suggest a homogeneous distribution of the monomer and dimer molecules. On the other hand, for DPPA multilayer films a demixing leading to a two-phase system is suggested. The phase separation during compression of the mixed DPPA monolayer forms regions where almost exclusively H-like dimers are present and regions where monomers and a small amount of quenching H-like dimers coexist.

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Section: Methodsmentioning

confidence: 82%

Section: Methodsmentioning

confidence: 99%

Global Analysis of the Fluorescence Decays of N,N‘-Dioctadecyl Rhodamine B in Langmuir−Blodgett Films of Diacylphosphatidic Acids

Ballet¹,

Auweraer²,

Schryver³

et al. 1996

J. Phys. Chem.

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“…Dipalmitoylphosphatidylcholine (DPPC) is the one that is difficult for multilayer LB deposition [19][20][21]. One possible strategy to enhance the poor LB transfer characteristic of a DPPC monolayer is to incorporate a transfer promoter as a mixed monolayer during deposition.…”

Section: Introductionmentioning

confidence: 99%

Thermodynamic characteristics and Langmuir–Blodgett deposition behavior of mixed DPPA/DPPC monolayers at air/liquid interfaces

Lee

Lin

Chang

2006

Journal of Colloid and Interface Science

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“…The obvious alternative is to prepare lipid or protein-lipid bilayers on solid supports, a task that many experimentalists have found to be rather complicated. Early attempts to create lipid bilayer structures of natural phospholipids using the Langmuir-Blodgett method were not always successful (Roberts, 1990;Sellström et al, 1992). A typical experience is that the first monolayer is easily deposited; however, during the dipping procedure to produce the second layer, the first layer was stripped off.…”

Section: Introductionmentioning

confidence: 99%

From Liposomes to Supported, Planar Bilayer Structures on Hydrophilic and Hydrophobic Surfaces: An Atomic Force Microscopy Study

Jaß¹,

Tjärnhage

Puu

2000

Biophysical Journal

Self Cite

281

262

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The sequence of events involved in the transition from attached liposomes to bilayer patches on hydrophilic and hydrophobic solid supports were visualized in situ by Tapping Mode atomic force microscopy in liquid. In a smooth manner, the attached liposomes spread and flattened from the outer edges toward the center until the two membrane bilayers were stacked on top of each other. The top bilayer then either rolls or slides over the bottom bilayer, and the adjacent edges join to form a larger membrane patch. This is clearly visible from the apparent height of 6.0-7.5 nm of the single bilayer, measured in situ. The addition of calcium appeared to increase the rate of the processes preventing the visualization of the intermediate stages. The same intermediate steps appeared to be present on hydrophobic surfaces, although the attached liposomes seemed to be distorted and the resultant membrane edges were uneven. This work has provided visual and detailed information on liposome coalescence (fusion) onto solid supports and demonstrated how the atomic force microscope can be used to study the process.

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On the deposition of phospholipids onto planar supports with the Langmuir—Blodgett technique using factorial experimental design 1. Screening of various factors and supports

Cited by 13 publications

References 21 publications

Global Analysis of the Fluorescence Decays of N,N‘-Dioctadecyl Rhodamine B in Langmuir−Blodgett Films of Diacylphosphatidic Acids

Global Analysis of the Fluorescence Decays of N,N‘-Dioctadecyl Rhodamine B in Langmuir−Blodgett Films of Diacylphosphatidic Acids

Thermodynamic characteristics and Langmuir–Blodgett deposition behavior of mixed DPPA/DPPC monolayers at air/liquid interfaces

From Liposomes to Supported, Planar Bilayer Structures on Hydrophilic and Hydrophobic Surfaces: An Atomic Force Microscopy Study

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