On the determination of protein-bound hexosamines in serum, with particular reference to amyloidosis

Seventeen strains of oral bacteria of the genera Actinomyces (5), Bacteroides (3), and Streptococcus (9) were tested for aggregation by the human whole salivary mucin fraction (HWSM) in comparison to three types of animal mucin preparations from submandibular glands of cow (BSM) and sheep (OSM), and from the stomach of pig (PGM). Considerable variation was seen with respect to the rate and titer of aggregation induced by these mucins. The aggregating activity of HWSM varied widely among the different bacterial strains. The Bacteroides group showed hardly any induced aggregation, whereas the final aggregation titers varied for S. sanguis (3 strains) between 12 and 48, for S. oralis (3 strains) between 6 and 48, for the S. mutans group (3 strains) between 6 and 96, and for the five Actinomyces strains even between 6 and 192. For a particular strain, similar differences in titer were seen between the four mucins. For a human salivary mucin (MG-2) it has been described that sialic acid in the sequence NeuAc (alpha 2,3)Gal(beta 1,3)GalNac- was specifically involved in the interaction with S. sanguis strains, in contrast to S. rattus BHT. Our results, however, indicate that this sugar sequence is not a prerequisite for the aggregation of S. sanguis, as animal mucins, devoid of this structure, were equally well or even better capable of inducing aggregation. On the other hand, desialization of BSM and OSM largely abolished their aggregating capability towards S. rattus BHT. Moreover, it was found that BSM and OSM, which are comparable with respect to their major oligosaccharide structure, show considerable differences in aggregating activity towards the same bacterial strain.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

Aggregation of oral bacteria by human salivary mucins in comparison to salivary and gastric mucins of animal origin

Koop

Valentijn-Benz

Amerongen

et al. 1990

Antonie van Leeuwenhoek

View full text Add to dashboard Cite

show abstract

Isolation, purification and chemical characterization of mucins from human submandibular glands

Oemrawsingh

Roukema

1974

Archives of Oral Biology

View full text Add to dashboard Cite

Composition and biological properties of mucins, isolated from human submandibular glands

Oemrawsingh

Roukema

1974

Archives of Oral Biology

View full text Add to dashboard Cite

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

On the determination of protein-bound hexosamines in serum, with particular reference to amyloidosis

Cited by 6 publications

References 29 publications

Aggregation of oral bacteria by human salivary mucins in comparison to salivary and gastric mucins of animal origin

Aggregation of oral bacteria by human salivary mucins in comparison to salivary and gastric mucins of animal origin

Isolation, purification and chemical characterization of mucins from human submandibular glands

Composition and biological properties of mucins, isolated from human submandibular glands

Contact Info

Product

Resources

About