On the dielectric relaxation of biological cell suspensions: The effect of the membrane electrical conductivity

Biasio, A. Di; Cametti, C.

doi:10.1016/j.colsurfb.2011.01.038

Cited by 22 publications

(23 citation statements)

References 29 publications

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“…2(c), with the most significant change due to the membrane conductivity in the range 10 À4 S/m-10 À3 S/m. This trend is consistent with theoretical predictions, 25 which shows that a membrane conductivity smaller than 10 À5 S/m has little influence on the impedance data, whilst for conductivities greater than 10…”

Section: Resultssupporting

confidence: 80%

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Microfluidic impedance cytometry of tumour cells in blood

2014

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The dielectric properties of tumour cells are known to differ from normal blood cells, and this difference can be exploited for label-free separation of cells. Conventional measurement techniques are slow and cannot identify rare circulating tumour cells (CTCs) in a realistic timeframe. We use high throughput single cell microfluidic impedance cytometry to measure the dielectric properties of the MCF7 tumour cell line (representative of CTCs), both as pure populations and mixed with whole blood. The data show that the MCF7 cells have a large membrane capacitance and size, enabling clear discrimination from all other leukocytes. Impedance analysis is used to follow changes in cell viability when cells are kept in suspension, a process which can be understood from modelling time-dependent changes in the dielectric properties (predominantly membrane conductivity) of the cells. Impedance cytometry is used to enumerate low numbers of MCF7 cells spiked into whole blood. Chemical lysis is commonly used to remove the abundant erythrocytes, and it is shown that this process does not alter the MCF7 cell count or change their dielectric properties. Combining impedance cytometry with magnetic bead based antibody enrichment enables MCF7 cells to be detected down to 100 MCF7 cells in 1 ml whole blood, a log 3.5 enrichment and a mean recovery of 92%. Microfluidic impedance cytometry could be easily integrated within complex cell separation systems for identification and enumeration of specific cell types, providing a fast in-line single cell characterisation method.

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Section: Resultssupporting

confidence: 80%

“…The cell membrane is an excellent electrical insulator [23][24][25][26] and at low frequencies cells behave as insulating particles. At higher frequencies (beyond the relaxation time of the membrane), the membrane becomes electrically transparent allowing the intracellular properties to be probed.…”

Section: Resultsmentioning

confidence: 99%

Microfluidic impedance cytometry of tumour cells in blood

2014

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“…13 Since, impedance is the opposition to the flow of AC in a complex system, AC measurements provide a number of additional cell parameters including membrane capacitance and conductivity, and cytoplasmic conductivity. [11][12][13] These reflect cell type, membrane integrity, morphology, cytoplasmic complexity, cell cycle phase, and viability. 13 Fig 1 and 2 show the frequency dependence of the relative permittivity ε' and the loss factor ε"of melanoma cells.…”

Section: Resultsmentioning

confidence: 99%

“…11,12 It is based on the interaction of an external field with the electric diopole moment of the sample, often expressed by permittivity. 12 It has ability to characterize the electrochemical properties of biological systems by measuring the impedance of a system over a range of frequencies, and therefore the frequency response of the system, including the energy storage and dissipation properties, is revealed.…”

Section: Resultsmentioning

confidence: 99%

“…When an electric current in a specific range of frequencies is applied, cells may have a dielectric relaxation due to structural or counter ion relaxation. 10,11 This relaxation may change the phenotype of cells, in turn, give enhanced migrational characteristics to the cells. 12 In this study, both primary and metastatic melanoma cells that have pleomorphic with strong invasive characteristics were chosen, and the changes in the morphology and the migrational behavior of these cells at the characteristic frequencies for a potential dielectric relaxations have been evaluated.…”

Section: Introductionmentioning

confidence: 99%

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Dielectric response of human melanoma cells on the surface of polymeric chemoattractants

Beard

Kim

Roh

et al. 2012

Tissue Eng Regen Med

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The influences of dielectric properties on the malignant cell adhesion and migration have been investigated. The Wistar Melanomas, primary (WM793) and metastatic (WM1205), respectively, were chosen as the model cell lines because of their pleomorphic and invasive characteristics, and the dielectric relaxation frequencies of the cells were determined using the impedance spectroscopy. Both WM793 and WM1205 showed the enhanced adhesion and the interesting changes in their morphology under the dielectric stimulations. However, it was found that the changed conditions of cell adhesion due to dielectric relaxation did not influence the migrational behavior of the cells.

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Computational Approaches

Brosseau

2023

Studies in Mechanobiology, Tissue Engineering and Biomaterials

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On the dielectric relaxation of biological cell suspensions: The effect of the membrane electrical conductivity

Cited by 22 publications

References 29 publications

Microfluidic impedance cytometry of tumour cells in blood

Microfluidic impedance cytometry of tumour cells in blood

Dielectric response of human melanoma cells on the surface of polymeric chemoattractants

Computational Approaches

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