On the electromigration of charged fluorophore‐labeled oligosaccharides in polyethylene oxide solutions

Abstract

The separation mechanism of charged fluorophore (aminopyrenetrisulfonate)-labeled maltooligosaccharides with α1-4 linkages was studied in polyethylene oxide (PEO) solutions (MW 300 000 Da) with special interest to possible analyte and/or network deformations as well as potential solute-matrix interactions. The electrophoretic mobilities of the 8-aminopyrene-1,3,6-trisulfonate-labeled maltooligosaccharides were found proportional with their MW(-2/3) . The Arrhenius function was used to determine the activation energy needed by the labeled sugars to migrate through the separation media. With increasing solute size, the activation energy (Ea ) values decreased in polymer concentrations above the entanglement threshold of the PEO, while showed apparently independent function at the entanglement threshold. The observed phenomenon was considered as a result of solute-matrix interaction, which could be alleviated by the addition of an organic modifier to the BGE.

Profiling the N-Glycan Composition of IgG with Lectins and Capillary Nanogel Electrophoresis

“…In agreement with results reported by others, ,, decreasing the separation temperature increased the migration time of all of the N -glycan peaks (see Table S-2). A plot of the mobility versus temperature (Figure S-3) revealed nominal change in mobility below 23 °C.…”

Recent advances in microscale separation techniques for glycome analysis

“…This technology has enabled different types of glycomic analysis, such as glycoproteins [80,81], proteoglycans [82], GAGs [83,84], and glycosphingolipids [57]. To enhance the resolution for glycans, strategies have been adopted to manipulate their mobilities, such as using viscosity modifiers and polymer matrix additives [85,86], temperature adjustments [87,88], affinity materials [89][90][91] (like chemical moieties, enzymes or lectins), and so on. For glycan detection, there are innovative label-free detection methods reported by Kubo et al [92] and Marie et al [93], respectively.…”

Database search assisted N-glycan structure identification

“…In a recent paper [17], Jarvas et al studied the electromigration of charged fluorophore-labeled oligosaccharides in polyethylene oxide solutions utilizing the activation energy concept. The authors published evidence on the solute-matrix interaction, and interestingly found that it could be alleviated by the addition of an organic modifier to the background electrolyte.…”

“…The authors published evidence on the solute-matrix interaction, and interestingly found that it could be alleviated by the addition of an organic modifier to the background electrolyte. Other work from the Guttman group focused on the electromigration of oligosaccharides in viscosity modifier and polymeric additive containing background electrolytes [17,18]. Activation energy curves were constructed based on the slopes of the Arrhenius plots from CE separation of APTS-labeled maltooligosaccharides in the temperature range of 20-50°C.…”

“…Deposition of any sample or sample matrix molecules, microscopic, and macroscopic change in the composition of the background electrolyte, alteration of the pH or temperature may occur such surface charge heterogeneity. Furthermore, the apparent electrophoretic mobility may be further influenced by solute-matrix interactions [17]. Conversion of measured migration times to a kind of relative migration times can increase precision, which in the practice led to the utilization of the so-called glucose unit approach.…”