The bubble column performance can change significantly as a result of flow regime change. Since reactor volume productivity, mass and heat transfer as well as mixing are affected by the prevailing flow regime, it is very important to know how to identify it. In this work, flow regime identification was performed on the basis of the Kolmogorov entropy (KE) algorithm applied to nuclear gauge densitometry data. In addition, the average cycle time was used for validation of the results. Three transition velocities were identified that delineated the boundaries of the three main hydrodynamic regimes. The first two transition points were also confirmed by the information entropy concept. The increasing KE trend in the bubbly flow regime and the decreasing KE trend in the churn-turbulent regime were predicted successfully by means of new semi-theoretical models.