Abstract. We provide splitting formulas for certain Waldhausen Nil-groups. We focus on Waldhausen Nil-groups associated to acylindrical amalgamations Γ = G 1 * H G 2 of groups G 1 , G 2 over a common subgroup H. For these amalgamations, we explain how, provided G 1 , G 2 , Γ satisfy the Farrell-Jones isomorphism conjecture, the Waldhausen Nil-groupscan be expressed as a direct sum of Nil-groups associated to a specific collection of virtually cyclic subgroups of Γ. A special case covered by our theorem is the case of arbitrary amalgamations over a finite group H.