On the Fate of Plastid DNA Molecules during Leaf Development: Response to the Golczyk et al. Commentary

Oldenburg, Delene J.; Rowan, Beth A.; Kumar, Rachana A.; Bendich, Arnold J.

doi:10.1105/tpc.113.121772

Cited by 17 publications

(18 citation statements)

References 22 publications

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“…However, whether DNA is degraded nucleolytically has been controversial, partly because of technical limitations of qPCR, variation within species and tissues, and a lack of mechanistic insights 16, 17,41 . Our studies with DPD1 uncovered the prevailing degradation mechanism among seed plants.…”

Section: Discussionmentioning

confidence: 99%

“…Excess ptDNA can be dispensable without affecting organelle functionality or cell viability, potentially providing a source of organic P for relocation when degraded. However, whether the amount of cpDNA/ptDNA is controlled by degradation in mature leaves has long been unclear 16,17 . Little is known about the enzymatic degradation mechanism and its possible impact on the efficient use of the internal P pool in endosymbiotic organelles.…”

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confidence: 99%

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Organelle DNA degradation contributes to the efficient use of phosphate in seed plants

et al. 2018

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Organelle DNA degradation contributes to the efficient use of phosphate in seed plants

et al. 2018

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“…Because of the size and complexity of plant mitochondrial genomes, the exact mechanism for plant mtDNA replication remains unclear. Organellar DNA levels vary significantly in different ages and tissues depending on energy needs, tissue type, and stage of development (34)(35)(36). This suggests tightly controlled regulation of mtDNA and ctDNA replication and genome copy number.…”

Section: Replication Mechanisms Of Plant Mtdnamentioning

confidence: 99%

Plant mitochondrial DNA

Nielsen¹

2017

Front Biosci

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Sitmmary. DNA was isolated from a mitochondrial fraction of each of the following plant materials: Mung bean (Phascolus aureuis) etiolated hypocotyl; turnip (Brassica rapa) root; sweet wtato (Iponioea batatas) root; and onion (Allium cepa) bulb. It was found that all of these nitochondrial fractions contained DNA, the densities of which were identical (p=1.706 gcm3). An additional DNA (p=1.695) band found in the mitochondrial fraction of Birassica rapa, was identical to DNA separately isolated from the chloroplast-rich fraction. The origin of the second DNA from Alliutm mito-chondrial fraction was not identified. Contrary to the identity of the mitochondrial DNA, DNA from nuclear fractions differed not only with each other but fronm the corresponding mitochondrial DNA. DNA from Phaseoluts and Brassica mitochondria showed the hyperchromicity characteristic of double strainded, native DNA upon heating; Tm's in 0.0195 Na+ were the same; 72.00. The amount of DNA within the mitoch!ondrion of Phascolus was estimated to be 5.0 X 1010 ug; this estimate was made by isolating the mitochondrial DNA concomitantly with the known amiount of added 15N2H B. subtilis DNA (p=1.740). Approximately the samiie amount of DNA was presenit in the mitochondrion of Brassica or Ipomoea. The presence of mitochondrial DNA has been postulated from various experimental observations, such as the incorporation of 3H-thymidine (1, 14), Feulegen staining bodies (1) and most extensively, the DNA-like fibrils resolved by the electromicro-scopic studies made with various organisms (8). Early genetic studies obtained with "petite" in yeast (2) and "poky" in Neurospora (see 19), together with the demonstration by Luck (4) that the mitochondrion is an autonomous replicating unit, may be well reconciled with the idea that mitochondria contain DNA which carries essential information necessary for the function and the replication of the mito-chondria. To support this notion, Luck and Reich (5) succeeded not only in isolating DNA from Neu-rospora mitochondria, but also in demonstrating that this DNA possibly serves as a template for RNA synthesis within the mitochonid(rioni. Subsequent studies carried out in our laboratory with Tetrahyinienia and Paramecium confirmed the presence of DNA within mitochondria in these organisms and showed that mitochondrial DNA in these organismiis markedly differ not only from the respective niuclear DNA, but with each other (18). According to the extensive analysis by Sueoka (15, 16) of DNA of various organisms, cellular DNA reflects the phylogenetic relations as the mean base compositions of DNA from related organisms are similar. It 1 This work was supported by grants from the National Science Foundation. was originally hoped that mitochondrial DNA woulcl exhibit such a relationship. The contrary results obtained in protozoa have prompted us to extend this kind of analysis to mitochondria of different organisms. This paper reports that among plant species the DNA of the mitochondria are essentially identical in terms of t...

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“…c; Oldenburg et al . ). Experiments involving Arabidopsis mutants suggest that plastome instability because of ROS damage leads to inhibition of DNA repair proteins and to declined production and malfunction of critical proteins encoded by the plastome.…”

Section: Introductionmentioning

confidence: 97%

“…Such a decline, due to fragmentation, mutations and loss of function of plastomes, is attributed to the cumulative oxidative damage suffered over the entire life span of the leaf tissue ( Fig. 1c; Oldenburg et al 2014). Experiments involving Arabidopsis mutants suggest that plastome instability because of ROS damage leads to inhibition of DNA repair proteins and to declined production and malfunction of critical proteins encoded by the plastome.…”

Section: Introductionmentioning

confidence: 99%

Do cytokinins, volatile isoprenoids and carotenoids synergically delay leaf senescence?

Dani

Fineschi

Michelozzi

et al. 2016

Plant Cell & Environment

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On the Fate of Plastid DNA Molecules during Leaf Development: Response to the Golczyk et al. Commentary

Cited by 17 publications

References 22 publications

Organelle DNA degradation contributes to the efficient use of phosphate in seed plants

Organelle DNA degradation contributes to the efficient use of phosphate in seed plants

Plant mitochondrial DNA

Do cytokinins, volatile isoprenoids and carotenoids synergically delay leaf senescence?

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