On the Fatty Acids of <i>Brucella abortus</i> and <i>Brucella melitensis</i>

Thiele, O. W.; Lacave, Ch.; Asselineau, J.

doi:10.1111/j.1432-1033.1969.tb19621.x

Cited by 37 publications

(12 citation statements)

References 25 publications

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“…The analytical data (IR, 1 H and 13 C NMR, MS) 19;20 of 3 and 4 were in accord with previous structural assignments. 16 The location of the keto group follows clearly from the mass spectral fragmentation patterns as reported initially by Thiele et al 21 and these assignments have been confirmed through degradation experiments. 16 The molar rotation of each ketone was evaluated and compared to the corresponding value obtained by Tocanne 17 for the related compounds 5 and 6 (Scheme 2).…”

Section: Resultssupporting

confidence: 61%

The absolute configuration of methyl dihydrosterculate: an unusual phytofatty acid isolated from the seed oil of Litchi chinensis

Stuart

Buist

2004

Tetrahedron: Asymmetry

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Section: Resultssupporting

confidence: 61%

The absolute configuration of methyl dihydrosterculate: an unusual phytofatty acid isolated from the seed oil of Litchi chinensis

Stuart

Buist

2004

Tetrahedron: Asymmetry

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“…After reutilization of poly(3-hydroxybutyric inid) this relation was reversed : an increase of hexadecanoic acid, a decrease of a C,, cyclopropane acid. The occurrence of a C,, cyclopropane acid is tentatively suggested on the basis of its retention time as compared with C,, cyclopropane acid found in Brucellae previously studied [51]. No evidence for hydroxy fatty acids has been obtained.…”

Section: Spot 1 Of the Neutral Lipids Of Growth Phase Imentioning

confidence: 89%

The “Free” Lipids of Two Different Strains of Hydrogen‐Oxidizing Bacteria in Relation to Their Growth Phases

Thiele

Dreysel

Hermann³

1972

European Journal of Biochemistry

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Two strains of hydrogen‐oxidizing bacteria were used: Hydrogenomonas eutropha strain H16 and strain 11/x. The former is a gram‐negative flagellated rod and has been relatively well known with respect to its morphological, physiological, and biochemical properties. The latter is a gram‐positive, non spore‐forming, motile small rod the definite classification of which is unknown as yet. Both strains were grown chemolithotrophically in submerged culture with a limited nitrogen‐source and supplied with a gas mixture of H2, O2, and CO2. The lipid composition of the bacteria was determined at the end of the exponential phase (I), at the storage phase (II), and after reutilization of storage material (III). Along with the accumulation of poly(3‐hydroxybutyric acid) as the major storage material in strain H16 the amount of other lipids was shown to increase 1.8‐fold at phase II and to decrease at phase III. There is little or no change in the lipid pattern in various growth phases. It is suggested that the 1.8‐fold increase of the latter lipids during phase II is due to the synthesis of membrane lipids that are required for the formation of membranes surrounding the intra‐cellular granules of accumulating poly(3‐hydroxybutyric acid). In strain 11/x the amount of lipids increases 7 times along with an equal increase of carbohydrates at phase II. The majority of accumulated lipids consists of triglycerides free of significant amounts of unusual acyl group. It is suggested that there is a true storage of triglycerides that are reutilized during phase III. The lipid pattern of strain H16 is characterized by a high proportion of “strongly bound lipids” that consist predominantly of phospholipids. Characteristic lipid components are phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol, while coenzyme Q8 (0.41 μmol/g bacterial dry weight) has been found among the neutral lipids. A C17 cyclopropane acid (probably cis‐9,10‐methylene hexadecanoic acid) is present in all saponifiable lipids. The lipid pattern of strain 11/x is characterized by a high proportion of “loosely bound lipids” that consist predominantly of neutral lipids, particularly of triglycerides. Among the polar lipids, diphosphatidylglycerol and phosphoinositides are most abundant, and several unknown phospholipids are also present. No cyclopropane fatty acid has been found. The possible use of strain 11/x as a source of nutritional fat is discussed.

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“…The application of CFA analysis in studying another formerly unclassified group (IVe) proved useful in establishing proper classification of the urinary tract pathogen Ohigella urealytica (35 (28,43,140).…”

Section: Identification To Genus or Speciesmentioning

confidence: 99%

Applications of cellular fatty acid analysis

1991

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More than ever, new technology is having an impact on the tools of clinical microbiologists. The analysis of cellular fatty acids by gas-liquid chromatography (GLC) has become markedly more practical with the advent of the fused-silica capillary column, computer-controlled chromatography and data analysis, simplified sample preparation, and a commercially available GLC system dedicated to microbiological applications. Experience with applications in diagnostic microbiology ranges from substantial success in work with mycobacteria, legionellae, and nonfermentative gram-negative bacilli to minimal involvement with fungi and other nonbacterial agents. GLC is a good alternative to other means for the identification of mycobacteria or legionellae because it is rapid, specific, and independent of other specialized testing, e.g., DNA hybridization. Nonfermenters show features in their cellular fatty acid content that are useful in identifying species and, in some cases, subspecies. Less frequently encountered nonfermenters, including those belonging to unclassified groups, can ideally be characterized by GLC. Information is just beginning to materialize on the usefulness of cellular fatty acids for the identification of gram-positive bacteria and anaerobes, despite the traditional role of GLC in detecting metabolic products as an aid to identification of anaerobes. When species identification of coagulase-negative staphylococci is called for, GLC may offer an alternative to biochemical testing. Methods for direct analysis of clinical material have been developed, but in practical and economic terms they are not yet ready for use in the clinical laboratory. Direct analysis holds promise for detecting markers of infection due to an uncultivable agent or in clinical specimens that presently require cultures and prolonged incubation to yield an etiologic agent.

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On the Fatty Acids of Brucella abortus and Brucella melitensis

Cited by 37 publications

References 25 publications

The absolute configuration of methyl dihydrosterculate: an unusual phytofatty acid isolated from the seed oil of Litchi chinensis

The absolute configuration of methyl dihydrosterculate: an unusual phytofatty acid isolated from the seed oil of Litchi chinensis

The “Free” Lipids of Two Different Strains of Hydrogen‐Oxidizing Bacteria in Relation to Their Growth Phases

Applications of cellular fatty acid analysis

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