On the formation of coprosterol in the intestine

Snog-Kjaer, A.; Prange, I.; Dam, Henrik

doi:10.1007/bf02158396

Cited by 7 publications

(2 citation statements)

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“…H. Danielsson, W. Klyne, S. Lieberman, and M. Gut, respectively. Coprostanol was prepared microbiologically from cholesterol by intestinal microorganisms in wit70 [6,8].…”

Section: Reference Compounbmentioning

confidence: 99%

Identification of 22‐, 24‐ and 26‐Hydroxycholesterol in the Steroid Sulphate Fraction of Faeces from Infants

Gustafsson

Sjövall

1969

European Journal of Biochemistry

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A fraction of solvolyzable sterol conjugates with the chromatographic properties of steryl sulphates was isolated from pooled faeces collected from infants 1-4 and 6-12 months old. The major sterols in this fraction were analyzed by gas chromatography-mass spectrometry. The pool from the younger infants was shown to contain cholesterol and 22a-, 24&, and 26-hydroxycholesterol and the pool from the older infants contained cholesterol, campesterol, /?-sitosterol, and 22a-, and 24~-hydroxycholesterol. The concentrations of cholesteryl and 24-hydroxycholesteryl sulphates were higher in faeces from the older than from the younger subjects.I n a series of papers we have described the identification of steroids in faeces from germfree and conventional rats (for references see [l]). These studies have shown a pronounced difference between the two types of animals in the composition and nature of the steroid metabolites excreted. Evidence has been presented for the appearance of bacterial metabolites in the urine [2]. The pronounced effect of the intestinal bacteria on the steroid excretion in rats suggests that the establisment of an intestinal flora could influence the nature of steroid metabolites and their route of excretion in the newborn infant. A striking change in the urinary steroid pattern during the first year is the decrease of 16a-hydroxysteroids [3] and one of the important bacterial reactions in the rat is the removal of the 16a-hydroxy group in C,,. It is therefore of interest to investigate the nature of steroids in faeces from infants during the first year of life. Since most of the steroids in infant urine occur as sulphates [5] the sulphate fraction in faeces has been investigated. As shown in the present paper a major group of compounds in this fraction consists of C,,O, steroids, the identification of which will be described. MATERIALS AND METHODS Collection of FaecesFaeces were collected in polythene vials or plastic bags from two groups of infants (both sexes): 1 to 4 months old (yielding faecal pool PI), and 6 to 12 months of age (yielding pool PII). Pool PI, obtained from 28 subjects, consisted of 285 g of faeces; poolPII (from 10 subjects) weighed 225g. The samples were stored a t -20" until analyzed. The infants were kept on different regular diets (see [S]).

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