<p style='text-indent:20px;'>This work establishes the parallel between the properties of classic elliptic PDEs and the one-sided 1-D fractional diffusion equation, that includes the characterization of fractional Sobolev spaces in terms of fractional Riemann-Liouville (R-L) derivatives, variational formulation, maximum principle, Hopf's Lemma, spectral analysis, and theory on the principal eigenvalue and its characterization, etc. As an application, the developed results provide a novel perspective to study the distribution of complex roots of a class of Mittag-Leffler functions and, furthermore, prove the existence of real roots.</p>