On the hydrodynamic analysis of conformation in mixed biopolymer systems

Harding, Stephen E.; Abdelhameed, Ali S.; Morris, Gordon A.

doi:10.1002/pi.2934

Cited by 24 publications

(6 citation statements)

References 27 publications

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“…The relationship of M w with radius of gyration ( R g ) was established using linear regression, and the slope of R g - M w could represent the chain conformation of polymers. The slope of rigid chain in solvent is higher than 1.0, and the values of 0.33 and 0.5–0.6 represent the conformation of sphere chain and random coil, respectively [ 36 ]. The conformation plot slopes of KSP-30E and NSP-30E ( Figure 3 C) were 1.10 and 1.15, indicating the rigid chain conformation of these polysaccharides [ 37 ].…”

Section: Resultsmentioning

confidence: 99%

Research on Extraction, Structure Characterization and Immunostimulatory Activity of Cell Wall Polysaccharides from Sparassis latifolia

Liu

Zhang

et al. 2022

Polymers

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The cell wall polysaccharides were extracted from Sparassis latifolia fruit bodies by acid–alkali and superfine-grinding assisted methods, and the chemical characterization and in vitro immunity activities of these polysaccharide fractions were studied and compared. Results showed that superfine-grinding assisted extraction exhibited the highest yield of polysaccharides (SP, 20.80%) and low β-glucan content (19.35%) compared with alkaline extracts. The results revealed that the 20% ethanol precipitated fraction (20E) from SP was mainly composed of β-(1→3)-glucan and α-(1→4)-glucan. With the increase of ethanol precipitation, the fractions (30E, 40E, 50E) were identified as α-(1→4)-glucan with different molecular weights and conformations. Cell wall polysaccharides extracted through NaOH (NSP) and KOH (KSP) extraction had similar yields with 8.90% and 8.83%, respectively. Structural analysis indicated that the purified fraction from KSP (KSP-30E) was a β-(1→3)-glucan backbone branched with β-(1→6)-Glcp, while the purified fraction from NSP (NSP-30E) mainly contained β-(1→3)-glucan with a small number of α-linked-Glcp. The two fractions both exhibited rigid chain conformation in aqueous solutions. All polysaccharide fractions exerted the activity of activating Dectin-1 receptor in vitro, and the KSP-30E mainly identified as β-(1→3)-glucan with the terminal group via 1→6-linkage attached at every third residue exhibited a stronger enhancing effect than other fractions. Results suggested that KOH extraction could be efficient for the preparation of bioactive β-(1→3, 1→6)-glucan as a food ingredient.

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Section: Resultsmentioning

confidence: 99%

Research on Extraction, Structure Characterization and Immunostimulatory Activity of Cell Wall Polysaccharides from Sparassis latifolia

Liu

Zhang

et al. 2022

Polymers

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“…Molecular weight (molar mass) and molar mass distribution have been documented as being the most important physical parameters affecting the immunogenicity of capsular polysaccharides 24 25 . While many other aspects of polysaccharide characterisation have been relatively thoroughly explored 26 27 28 , the physical characterisation of capsular polysaccharides (molecular size and mass distribution and conformational flexibility) has been less extensively pursued. Such physical studies for capsular polysaccharides have been generally limited to low pressure chromatographic analyses calibrated with “standards” and more recently to high performance size exclusion chromatography (SEC) coupled to on-line refractive index detector (RI), multi-angle light scattering (MALS).…”

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confidence: 99%

A glycoconjugate of Haemophilus influenzae Type b capsular polysaccharide with tetanus toxoid protein: hydrodynamic properties mainly influenced by the carbohydrate

Adams

Morris

Almutairi

et al. 2016

Sci Rep

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Three important physical properties which may affect the performance of glycoconjugate vaccines against serious disease are molar mass (molecular weight), heterogeneity (polydispersity), and conformational flexibility in solution. The dilute solution behaviour of native and activated capsular polyribosylribitol (PRP) polysaccharides extracted from Haemophilus influenzae type b (Hib), and the corresponding glycoconjugate made by conjugating this with the tetanus toxoid (TT) protein have been characterized and compared using a combination of sedimentation equilibrium and sedimentation velocity in the analytical ultracentrifuge with viscometry. The weight average molar mass of the activated material was considerably reduced (Mw ~ 0.24 × 106 g.mol−1) compared to the native (Mw ~ 1.2 × 106 g.mol−1). Conjugation with the TT protein yielded large polydisperse structures (of Mw ~ 7.4 × 106 g.mol−1), but which retained the high degree of flexibility of the native and activated polysaccharide, with frictional ratio, intrinsic viscosity, sedimentation conformation zoning behaviour and persistence length all commensurate with highly flexible coil behaviour and unlike the previously characterised tetanus toxoid protein (slightly extended and hydrodynamically compact structure with an aspect ratio of ~3). This non-protein like behaviour clearly indicates that it is the carbohydrate component which mainly influences the physical behaviour of the glycoconjugate in solution.

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“…In addition, GBP was found to be a linear polymer exhibiting a random coil conformation, demonstrated by a Mark–Houwink–Sakurada α value of 0.702. This interpretation is based on the accepted values of α for polymers, wherein the α value of 0.5–0.8 represents a malleable random coil conformation, a α value greater than 0.8 denotes semimalleable or extra stretched chains [ 45 ], a α value of 0 indicates rigid sphere, one of 2 demonstrates stiff rod, and one of ~0.7 specifies a linear conformation [ 46 ]. The linear structure of GBP was also evident from the Rg/Rh value (1.57), where this ratio was ~0.775 for globular polymers, and a higher value demonstrated a correspondingly greater deviation from an orbicular to a more linearly stretched conformation [ 47 ].…”

Section: Resultsmentioning

confidence: 99%

Antioxidant Potential of Physicochemically Characterized Gracilaria blodgettii Sulfated Polysaccharides

et al. 2021

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Marine rhodophyte polysaccharides have a wide range of described biological properties with nontoxic characteristics, and show great potential in prebiotics and the functional foods industries. However, there is a virtual lack of Gracilaria blodgettii polysaccharides (GBP) profiling and their bioactivities. This study was designed while keeping in view the lack of physical and chemical characterization of GBP. This polysaccharide was also not previously tested for any bioactivities. A linear random coil conformation was observed for GBP, which was found to be a polysaccharide. A significant sulfate (w/w, 9.16%) and 3,6-anhydrogalactose (AHG, w/w, 17.97%) content was found in GBP. The significant difference in its setting (27.33 °C) and melting (64.33 °C) points makes it resistant to increasing heat. This, in turn, points to its utility in industrial scale processing and in enhancing the shelf-life of products under high temperatures. A radical scavenging activity of 19.80%, 25.42% and 8.80% was noted for GBP (3 mg/mL) in 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis (ABTS) and hydroxyl radical (HO) scavenging assays, respectively. Therefore, the findings suggest that Gracilaria blodgettii polysaccharides display a good antioxidant potential and may have potential applications in the functional food industry.

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On the hydrodynamic analysis of conformation in mixed biopolymer systems

Cited by 24 publications

References 27 publications

Research on Extraction, Structure Characterization and Immunostimulatory Activity of Cell Wall Polysaccharides from Sparassis latifolia

Research on Extraction, Structure Characterization and Immunostimulatory Activity of Cell Wall Polysaccharides from Sparassis latifolia

A glycoconjugate of Haemophilus influenzae Type b capsular polysaccharide with tetanus toxoid protein: hydrodynamic properties mainly influenced by the carbohydrate

Antioxidant Potential of Physicochemically Characterized Gracilaria blodgettii Sulfated Polysaccharides

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