The research focuses on keystroke technique differences between pianists and organists. The keystroke characteristics (length and speed) during different hand/arm positions could reveal several differences in relation to the keyboard height. Considering the specifics of playing both instruments, the aim is the comparison of fine-motor parameters between pianists and organists. The assessed sample is repeated playing of C major scale using the right hand. The important criterion is the dynamics difference and its impact on the keystroke. The research was conducted with the keyboard STUDIO LOGIC 88 GRAND, enabling neutral conditions for independent measurement. Moreover, the software CUBASE PRO 9.5 was chosen for sample transformation to exact data. The research focused on the hand position in relation to the angle of its posture. The result validity was verified using the questionnaire. Particular differences in the finger technique of both groups were proved. Nevertheless, the same initial piano preparation leads to similar keystroke technique. The dynamics changing during playing the scale is neutral considering its quality. The differences in the spatial orientation change were revealed. The organ players were more flexible than the piano players when the keyboard position was changed. The similarities and differences of the keystroke technique of the organists and pianists are significantly influenced by the previous piano preparation in case of organists. Moreover, the fluent playing in the unusual keyboard height is best supported by the practical organ preparation. The research results can be used for the methodological and pedagogical purposes and for further scientific research.