On the Lipid Like Character of Prolamellar Bodies

Nordmann, Ursula; Tönissen, Hildegard; Bergweiler, Paul; Röper, Ursula

doi:10.1007/978-94-017-4971-8_138

Cited by 5 publications

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Ultrastructural localization of photosynthetic activity in thylakoids during chloroplast development in maize

Wrischer

1989

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The localization of photosynthetic activity in developing maize (Zea mays L.) chloroplasts was studied in situ by two electron-microscopic-cytochemical methods. The activity of photosystem I was detected by photooxidation of 3,3'-diaminobenzidine (DAB) and the activity of the photosystem II by photoreduction of thiocarbamyl nitrotetrazolium blue (TCNBT). During the transformation of proplastids into chloroplasts, at the base of the leaf blade the DAB reaction appeared before the TCNBT reaction. A positive DAB reaction was observed in the single thylakoids of plastids in cells located only about 0.5 mm above the base. Dark, osmiophilic DAB polymers accumulated in the lumina of the thylakoids. Plastid envelopes and tubules of the prolamellar bodies in immature chloroplasts were DAB-negative. In fully differentiated leaf tissue the DAB reaction was intense in the thylakoids of bundle-sheath chloroplasts, as well as in the stroma thylakoids and the peripheral grana thylakoids of mesophyll chloroplats. The photoreduction of TCNBT started in leaf tissue about 1 mm above the base. Dark granular material of reduced TCNBT appeared mostly in the partitions of grana, i.e. interthylakoidally, but some granules were also attached to the stroma thylakoids. The membranes of plastid envelopes and the tubules of prolamellar bodies showed a negative TCNBT reaction. Young bundle-sheath chloroplasts contained some reduced TCNBT in their grana; these deposits largely disappeared in the course of further differentiation. In mature leaf tissue the photoreduction of TCNBT was conspicuous in the grana of mesophyll chloroplasts, but very weak in the single thylakoids and in the granal rudiments of bundle-sheath chloroplasts.

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Ultrastructural localization of photosynthetic activity in thylakoids during chloroplast development in maize

Wrischer

1989

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Prolamellar-body structure, composition of molecular species and amount of galactolipids in etiolated, greening and reetiolated primary leaves of oat, wheat and rye

Kesselmeier

Laudenbach

1986

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The greening and reetiolation process of etiolated leaves of oat, wheat and rye, possessing different types of prolamellar bodies (PLBs), was observed by electron microscopy. Oat is known to possess unusual crystalline PLBs (so-called 'narrow type'). Rye and what, which normally show PLBs with more loosely packed tubules ('wide type') during etiolation, exhibited PLBs of the narrow type after illumination and subsequent reincubation in the dark (=reetiolation). Thus the reetiolated PLBs of wheat and rye did not differ from etiolated or reetiolated oat PLBs. In parallel with the microscopic analysis, intact leaves of all developmental stages were analysed for their galactolipid content and composition of molecular species using a newly developed high-performance liquid chromatography procedure. When oat, wheat and rye were compared, differences in the molecular species and the molar ratio of the two galactolipids monogalactosyldiacylglyceride (MGDG) and digalactosyldiacylglyceride (DGDG) were found. However, no parameter showed a correlation with PLB construction, disintegration or reconstruction. The results presented in this paper are not consistent with the hypothesis that the molar ratio of MGDG/DGDG is responsible for the tubular structure of prolamellar bodies in etioplasts.

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