On the location of 1-anilino-8-naphthalene-sulfonate in lipid model systems

Lesslauer, Werner; Cain, J. E.; Blasie, J. Kent

doi:10.1016/0005-2736(71)90054-x

Cited by 90 publications

(37 citation statements)

References 15 publications

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“…HC1 (Auramine O) were employed since their fluorescence is highly sensitive to the environment in which they are located. Ample evidence exists to assert that water-soluble ANS-binds at the membrane-water interface region [6][7][8], and its binding is sensitive to surface charges [4,9,10]. NPN, on the other hand, being neutral and largely water insoluble, is a probe of the more hydrophobic regions of the membrane [11 ].…”

Section: Introductionmentioning

confidence: 99%

Relation of membrane structural changes to energy spillover in oat and spinach chloroplasts: Use of fluorescence probes and light scattering

Vandermeulen

1974

Biochimica et Biophysica Acta (BBA) - Bioenergetics

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SUMMARYThe antagonism observed by Gross and Hess (Gross, E. and Hess, S. C. (1973) Arch. Biochem. Biophys. 159,[832][833][834][835][836] in the effect of monovalent and divalent cations on energy spillover between Photosystems I and II, as indicated by chlorophyll a fluorescence, was confirmed in spinach. Significant differences with oats, peas and lettuce were found using the same low salt method of preparation: the response to Na + and Ca 2+ was reversible, Ca 2÷ alone, when added first, produced a large (85 %) increase in the variable portion of the chlorophyll a fluorescence transient or the steady-state fluorescence, and Ca 2 + not only fully restored a Na ÷ induced inhibition of 680 nm fluorescence, but brought it above the original level. The fluorescence probes 1-anilinonaphthalene-8-sulfonate and N-phenyl-l-naphthylamine showed a rapid and a slower response, respectively, to the addition of mono-or divalent cations. Neither the structural changes responsible for the probe data, nor those leading to the measured cation stimulated changes in 90 ° light scattering could be precisely equated with any alteration of conformation involved in the chlorophyll spillover events.

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Section: Introductionmentioning

confidence: 99%

Relation of membrane structural changes to energy spillover in oat and spinach chloroplasts: Use of fluorescence probes and light scattering

Vandermeulen

1974

Biochimica et Biophysica Acta (BBA) - Bioenergetics

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“…It has been shown recently that the electron density profile of a bilayer can be calculated directly from continuous intensity data from dispersions of the bilayer by a deconvolution of its autocorrelation function (6). A higher-resolution electron density profile can be calculated from x-ray diffraction data from oriented multilayers by use of a reasonable phasing argument, which will be presented in this paper.…”

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confidence: 99%

X-Ray Diffraction Studies of Lecithin Bimolecular Leaflets with Incorporated Fluorescent Probes

Lesslauer

Cain

Blasie

1972

Proc. Natl. Acad. Sci. U.S.A.

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127

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X-ray diffraction studies on dispersions and oriented multilayers of dipalmitoyllecithin and different fluorescent probe molecules are reported. Analysis of the diffraction data provides the electron density profile for the different bilayers to 6-to 8Angstrom resolution. The location of the probe molecules in the bilayer structure and the perturbation of the lecithin bilayer produced by the probes are discussed.

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“…Accumulated evidence confirms the dominance of fluorescence from l -anilino-8-naphthalenesulphonate bound to lecithin-sphingomyelin pockets in natural membranes (19,20,21).…”

Section: Discussionmentioning

confidence: 65%

“…The binding of amphipathic molecules of 1-anilino-8-naphthalenesulphonate to lipid bilayers is assumed to involve insertion of the apolar naphthalene moiety into the hydrocarbön region, while the charged sulphonate moiety remains in the plane of the polar head groups (20,21,24). Thus, any enhanced lateral compressibility in the liquid crystalline state near the temperature of lipid phase transition (t^) could restilt in increased dye binding in this region, by allowing the insertion of l-anilino-S^naphthalenesulphonate into its membrane binding site with minimal perturbation (25).…”

Section: Discussionmentioning

confidence: 99%

1-Anilino-8-naphthalenesulphonate Binding Parameters in Red Cell Membranes. Does Diabetes Mellitus Affect Cell Membrane Dynamics?

Watała

Jóźwiak²

1989

Clinical Chemistry and Laboratory Medicine

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Summary:In this study we report the use of l-anilino-8-naphthalenesulphonate äs a fluorescence probe to investigate the properties of plasma membranes derived from normal and diabetic red blood cells.The binding of l-anilino-8-naphthalenesulphonate to diabetes-affected erythrocyte membranes, äs compared with controls, was measured by means of fluorescence polarization and fluorescence titration techniques.These measurements demonstrated anomalous l-anilino-^S-naphthalenesulphonate binding to pathological red cell membranes. The amount of l-anilino-8-naphthalenesulphonate bound to diabetic erythrocyte membranes was greater than that of controls. This fluorometric study indicated that the outer monolayer binding of l-anilino-8-naphthalenesulphonate was markedly augmented in the erythrocyte membranes of diabetic patients. Significant correlations were found between l-anilino-8-naphthalenesulphonate binding parameters and membrane lipid composition. The correlation between these binding parameters and non-enzymatic protein glycation was poor or moderate.Though the fluorescence intensity and emission maximum were quite similar in both groups investigated, binding studies reveäled that there were approximately 1.2 times the number of l-anilino-8-naphthalenesulphonate binding sites and a 33% increase in the K O value in diabetic membranes, suggesting significant differences in the environment of the l-anilino-8-naphthalenesulphonate binding sites in these two groups of patients.The results presented in this report indicate that (a) l-aniliüo-8-naphthalenesulphonate is a sensitive probe of membrane architecture alterations, and can be used to elucidate the perturbating effects of sterols in membrane Systems, and (b) that significant differences in membrane dynamics exist between normal and diabetic red cell membranes.The possibility that the increase in bound dye in diabetics was caused by enhanced lateral compressibility, or deiisity flüctuations, of whether it was due to additional binding sites at the boundary of heterogeneous lipid chisters is discussed. troductiono f fluorescence spectroscopy has been The revival of interest in fluorescence probes in recent used to probe many parameters of membrane strucyears has resulted in numerous studies dealing with ture using a variety of extrinsic probe molecules (2, thepertinehcepfselectedflüorophoresformonitoring 3), and several cheinicals have been especially dechanges of membrane structure and dynamics (l, 2), signed for this purpose. Amongst them, l-anilino-8-

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On the location of 1-anilino-8-naphthalene-sulfonate in lipid model systems

Cited by 90 publications

References 15 publications

Relation of membrane structural changes to energy spillover in oat and spinach chloroplasts: Use of fluorescence probes and light scattering

Relation of membrane structural changes to energy spillover in oat and spinach chloroplasts: Use of fluorescence probes and light scattering

X-Ray Diffraction Studies of Lecithin Bimolecular Leaflets with Incorporated Fluorescent Probes

1-Anilino-8-naphthalenesulphonate Binding Parameters in Red Cell Membranes. Does Diabetes Mellitus Affect Cell Membrane Dynamics?

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