On the natural selection and evolution of the aerobic phototrophic bacteria

Beatty, J. Thomas

doi:10.1007/1-4020-3324-9_97

Cited by 32 publications

(54 citation statements)

References 31 publications

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“…This phenomenon is particularly evident when cells are under nitrogen limitation and provided with a sole carbon source, and it results in PHA accumulation increasing with increasing light illumination, up to 10 millieinsteins/m 2 -s (8). It has been proposed that a mechanism may exist to regulate the photosynthesis and respiration processes (5). Such a mechanism can also be explained by assuming that PHA is a redox regulator in cellular metabolism (33).…”

Section: Discussionmentioning

confidence: 99%

“…As an AAPB strain, Dinoroseobacter sp. JL1447 can utilize light for additional energy to add to its heterotrophic metabolism, since the photosynthetic process shares the cellular respiration electron transfer system: with light, the NADH-dependent respiration is actually restrained, and thus NADH is saved (5,45), creating high NADH/NAD and PHB formation rates.…”

Section: Discussionmentioning

confidence: 99%

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Formation of Polyhydroxyalkanoate in Aerobic Anoxygenic Phototrophic Bacteria and Its Relationship to Carbon Source and Light Availability

Xiao

Jiao

2011

Appl Environ Microbiol

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Aerobic anoxygenic phototrophic bacteria (AAPB) are unique players in carbon cycling in the ocean. Cellular carbon storage is an important mechanism regulating the nutrition status of AAPB but is not yet well understood. In this paper, six AAPB species (Dinoroseobacter sp. JL1447, Roseobacter denitrificans OCh 114, Roseobacter litoralis OCh 149, Dinoroseobacter shibae DFL 12 T , Labrenzia alexandrii DFL 11 T , and Erythrobacter longus DSMZ 6997) were examined, and all of them demonstrated the ability to form the carbon polymer polyhydroxyalkanoate (PHA) in the cell. The PHA in Dinoroseobacter sp. JL1447 was identified as poly-betahydroxybutyrate (PHB) according to evidence from Fourier transform infrared spectroscopy, differential scanning calorimetry, and 1 H nuclear magnetic resonance spectroscopy examinations. Carbon sources turned out to be critical for PHA production in AAPB. Among the eight media tested with Dinoroseobacter sp. JL1447, sodium acetate, giving a PHA production rate of 72%, was the most productive carbon source, followed by glucose, with a 68% PHA production rate. Such PHA production rates are among the highest recorded for all bacteria. The C/N ratio of substrates was verified by the experiments as another key factor in PHA production. In the case of R. denitrificans OCh 114, PHA was not detected when the organism was cultured at C/N ratios of <2 but became apparent at C/N ratios of >3. Light is also important for the formation of PHA in AAPB. In the case of Dinoroseobacter sp. JL1447, up to a one-quarter increase in PHB production was observed when the culture underwent growth in a light-dark cycle compared to growth completely in the dark.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Formation of Polyhydroxyalkanoate in Aerobic Anoxygenic Phototrophic Bacteria and Its Relationship to Carbon Source and Light Availability

Xiao

Jiao

2011

Appl Environ Microbiol

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“…Nutrients concentrations were determined with a Skalar Autoanalyzer (Skalar Analytical B. V., The Netherlands) according to Wood et al (1967) and Bendschneider and Robinson (1952) for nitrate (NO 3 ) and nitrite (NO 2 ) concentrations and according to Murphy and Riley (1962) for phosphate (PO 4 ) concentrations. Protocols were adapted to the Skalar Autoanalyser from Tréguer and Le Corre (1975).…”

Section: Environmental Parametersmentioning

confidence: 99%

Ecology of aerobic anoxygenic phototrophic bacteria along an oligotrophic gradient in the Mediterranean Sea

et al. 2011

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Abstract. Aerobic anoxygenic phototrophic (AAP) bacteria are photoheterotrophic prokaryotes able to use both light and organic substrates for energy production. They are widely distributed in coastal and oceanic environments and may contribute significantly to the carbon cycle in the upper ocean. To better understand questions regarding links between the ecology of these photoheterotrophic bacteria and the trophic status of water masses, we examined their horizontal and vertical distribution and the effects of nutrient additions on their growth along an oligotrophic gradient in the Mediterranean Sea. Concentrations of bacteriochlorophyll-a (BChl-a) and AAP bacterial abundance decreased from the western to the eastern basin of the Mediterranean Sea and were linked with concentrations of chlorophyll-a, nutrient and dissolved organic carbon. Inorganic nutrient and glucose additions to surface seawater samples along the oligotrophic gradient revealed that AAP bacteria were nitrogen-and carbon-limited in the ultraoligotrophic eastern basin. The intensity of the Correspondence to: D. Lamy (lamyd2@univie.ac.at) AAP bacterial growth response generally differed from that of the total bacterial growth response. BChl-a quota of AAP bacterial communities was significantly higher in the eastern basin than in the western basin, suggesting that reliance on phototrophy varied along the oligotrophic gradient and that nutrient and/or carbon limitation favors BChl-a synthesis.

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“…A recent study suggested that AAP significantly contribute to bacterial production in oligotrophic oceans (17). Their ability to use light energy in addition to respiration could provide them an advantage over their heterotrophic bacterial relatives (2). In contrast, Schwalbach et al (32) found no effect of light on microbial community structure.…”

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confidence: 99%

Abundance, Depth Distribution, and Composition of Aerobic Bacteriochlorophyll a -Producing Bacteria in Four Basins of the Central Baltic Sea

Salka

Moulisová

Koblížek

et al. 2008

Appl Environ Microbiol

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The abundance, vertical distribution, and diversity of aerobic anoxygenic phototrophic bacteria (AAP) were studied at four basins of the Baltic Sea. AAP were enumerated by infrared epifluorescence microscopy, and their diversity was analyzed by using pufM gene clone libraries. In addition, numbers of CFU containing the pufM gene were determined, and representative strains were isolated. Both approaches indicated that AAP reached maximal abundance in the euphotic zone. Maximal AAP abundance was 2.5 ؋ 10 5 cells ml ؊1 (11% of total prokaryotes) or 1.0 ؋ 10 3 CFU ml ؊1 (9 to 10% of total CFU). Environmental pufM clone sequences were grouped into 11 operational taxonomic units phylogenetically related to cultivated members of the Alpha-, Beta-, and Gammaproteobacteria. In spite of varying pufM compositions, five clones were present in all libraries. Of these, Jannaschia-related clones were always found in relative abundances representing 25 to 30% of the total AAP clones. The abundances of the other clones varied. Clones potentially affiliated with typical freshwater Betaproteobacteria sequences were present at three Baltic Sea stations, whereas clones grouping with Loktanella represented 40% of the total cell numbers in the Gotland Basin. For three alphaproteobacterial clones, probable pufM phylogenetic relationships were supported by 16S rRNA gene analyses of Baltic AAP isolates, which showed nearly identical pufM sequences. Our data indicate that the studied AAP assemblages represented a mixture of marine and freshwater taxa, thus characterizing the Baltic Sea as a "melting pot" of abundant, polyphyletic aerobic photoheterotrophic bacteria.Aerobic bacteriochlorophyll a-producing bacteria, or so-called aerobic anoxygenic phototrophic bacteria (AAP), are strict aerobes, carrying out a photoheterotrophic metabolism. They require organic substrates for growth, but they can supplement a significant portion of their metabolic requirements by light-derived energy (8,16,26,31,39). AAP were discovered in the Bay of Tokyo by Shiba et al. (34) in 1970s. Later, their wide distribution in marine environments was documented by infrared (IR) kinetic fluorometry (16, 18), IR epifluorescence microscopy (IREM) (9,14,19,23,33) and pigment analyses (12), as well as cultivation-based (1, 15, 38) and genetic (3, 5, 13, 28, 41) studies. The planktonic bacterial assemblages are known to contain various AAP groups belonging to Alpha-, Beta-, and Gammaproteobacteria (41). Microscopic analyses have shown that AAP abundances vary in different environments from 0 to 24% of total prokaryotes (9, 21, 33).The ecological role of aerobic anoxygenic phototrophy is still unclear. A recent study suggested that AAP significantly contribute to bacterial production in oligotrophic oceans (17). Their ability to use light energy in addition to respiration could provide them an advantage over their heterotrophic bacterial relatives (2). In contrast, Schwalbach et al. (32) found no effect of light on microbial community structure. Clearly, more data on AA...

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On the natural selection and evolution of the aerobic phototrophic bacteria

Cited by 32 publications

References 31 publications

Formation of Polyhydroxyalkanoate in Aerobic Anoxygenic Phototrophic Bacteria and Its Relationship to Carbon Source and Light Availability

Formation of Polyhydroxyalkanoate in Aerobic Anoxygenic Phototrophic Bacteria and Its Relationship to Carbon Source and Light Availability

Ecology of aerobic anoxygenic phototrophic bacteria along an oligotrophic gradient in the Mediterranean Sea

Abundance, Depth Distribution, and Composition of Aerobic Bacteriochlorophyll a -Producing Bacteria in Four Basins of the Central Baltic Sea

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