On the presence of sulphate in pituitary lutropin

Effect of LH-RH on the Release of Sulfated Lutropin: A Potential In Vitro Bioassay for LH-RH.

“…However, the present state of knowledge of the specificity of the sulfation phenomenon (Green et al, 1985b) does emphasize that sulfate might play an important physiological role other than the expression of bioactivity at the receptor end (Sardanons et al, 1987). On the basis of our preliminary studies (Arunasmithasri et al, 1983) we had proposed that sulfation of LH might be a signal for its storage. We report here our results obtained with sheep pituitary cells incubated with 35SO2/4-and LH-RH showing that there is a dose dependent release of 35SO2/4-LH and it might serve as a potential in vitro bioassay for LH-RH.…”

“…Studies made during the last few years have established the fact that pituitary glycoprotein hormones are sulfated (Parsons and Pierce, 1980;Hortin et al, 1981;Anumula and Bahl, 1983). There have been a number of reports on the presence of sulfate in ovine (Anumula and Bahl, 1983), bovine (Hortin et al, 1981), human (Hortin et al, 1981) and rat (Arunasmithasri et al, 1983) LH (lutropin), on the structural requirements for sulfation and on the quantitative analysis Pierce, 1980 andRajender Kumar and of sulfate content (mol/ mol). In contrast there is less information on the physiological role of sulfate moiety in these hormones.…”