On the present and future of dissolution-DNP

Ardenkjær‐Larsen, Jan Henrik

doi:10.1016/j.jmr.2016.01.015

Cited by 221 publications

(347 citation statements)

References 47 publications

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“…9 In dissolution DNP, nuclear spins are polarized in the solid state and subsequently dissolved rapidly, resulting in a "hyperpolarized" room-temperature liquid yielding liquid-state signal enhancements of typically more than 10 000-fold. 9,10 Using this technology, samples containing low-γ nuclei such as 13 C may be both highly polarized and physiologically compatible, allowing for biomedical applications such as 13 C metabolic imaging in real-time. [11][12][13][14][15] The efficiency of DNP is highly dependent on the sample composition and physical conditions under which it takes place.…”

Section: Introductionmentioning

confidence: 99%

Influence of Dy3+ and Tb3+ doping on 13C dynamic nuclear polarization

Niedbalski

Parish

Kiswandhi

et al. 2017

The Journal of Chemical Physics

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Dynamic nuclear polarization (DNP) is a technique that uses a microwave-driven transfer of high spin alignment from electrons to nuclear spins. This is most effective at low temperature and high magnetic field, and with the invention of the dissolution method, the amplified nuclear magnetic resonance (NMR) signals in the frozen state in DNP can be harnessed in the liquid-state at physiologically acceptable temperature for in vitro and in vivo metabolic studies. A current optimization practice in dissolution DNP is to dope the sample with trace amounts of lanthanides such as Gd 3+ or Ho 3+ , which further improves the polarization. While Gd 3+ and Ho 3+ have been optimized for use in dissolution DNP, other lanthanides have not been exhaustively studied for use in 13 C DNP applications. In this work, two additional lanthanides with relatively high magnetic moments, Dy 3+ and Tb 3+ , were extensively optimized and tested as doping additives for 13 C DNP at 3.35 T and 1.2 K. We have found that both of these lanthanides are also beneficial additives, to a varying degree, for 13 C DNP. The optimal concentrations of Dy 3+ (1.5 mM) and Tb 3+ (0.25 mM) for 13 C DNP were found to be less than that of Gd 3+ (2 mM). W-band electron paramagnetic resonance shows that these enhancements due to Dy 3+ and Tb 3+ doping are accompanied by shortening of electron T 1 of trityl OX063 free radical. Furthermore, when dissolution was employed, Tb 3+ -doped samples were found to have similar liquid-state 13 C NMR signal enhancements compared to samples doped with Gd 3+ , and both Tb 3+ and Dy 3+ had a negligible liquid-state nuclear T 1 shortening effect which contrasts with the significant reduction in T 1 when using Gd 3+ . Our results show that Dy 3+ doping and Tb 3+ doping have a beneficial impact on 13 C DNP both in the solid and liquid states, and that Tb 3+ in particular could be used as a potential alternative to Gd 3+ in 13 C dissolution DNP experiments. Published by AIP Publishing.

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Section: Introductionmentioning

confidence: 99%

Influence of Dy3+ and Tb3+ doping on 13C dynamic nuclear polarization

Niedbalski

Parish

Kiswandhi

et al. 2017

The Journal of Chemical Physics

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“…[18][19][20][21] Many DNP optimization studies reported previously were performed at magnetic elds of 3.35 T, mainly due to the availability of commercial hyperpolarizer operating at 3.35 T (Hypersense, Oxford Instruments, UK) and W-band microwave source. Recent work has shown greater DNP-enhanced polarization levels can be gained at elds of 5-7 T 22-25 and a recent DNP hyperpolarizer for clinical purposes (SPINlab™, GE Healthcare, WI) has an operating eld of 5 T. 26 Therefore, greater exploration of suitable sample conditions for optimal polarization properties at these elds are needed, especially as the electronic properties of free radicals change with eld. 27 In this article, we have investigated the inuence of glassing solvent deuteration and the inclusion of Gd 3+ on 13 C DNP at a eld of 5 T. Free radicals 4-oxo-TEMPO and trityl OX063 ( Fig.…”

Section: Introductionmentioning

confidence: 99%

The effect of glassing solvent deuteration and Gd³⁺ doping on ¹³C DNP at 5 T

et al. 2016

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We report the influence of glassing solvent deuteration and Gd 3+ doping on 13 C dynamic nuclear polarization (DNP) nuclear magnetic resonance (NMR) performed on [1-13 C] sodium acetate at B 0 ¼ 5 T and 1.2 K. Our data reveal that at 5 T, glassing solvent deuteration still results in a 40% improvement of the 13 C DNP signal when a large electron spin resonance (ESR) linewidth 4-oxo-TEMPO free radical is used, but results in a 60% decrease of the DNP signal in the case of a sample doped with small ESR linewidth trityl OX063. An addition of a trace amount of the Gd 3+ complex Gd-HP-DO3A led to a negligible slight decrease on the 13 C polarization TEMPO-doped sample, but is still relatively beneficial for the trityl-doped sample with 30% improvement of the DNP-enhanced 13 C polarization. These findings indicate that while these DNP optimization steps are still valid at 5 T, the effects are not as pronounced as observed in 13 C DNP at B 0 ¼ 3.35 T. These DNP results at 5 T are discussed thermodynamically within the framework of the thermal mixing model of DNP.

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“…Tangible progress has been made towards the goals of developing affordable HP MRI biomedical applications and robust use of this technology in human studies [275,276]. …”

Section: Resultsmentioning

confidence: 99%

The use of hyperpolarized carbon-13 magnetic resonance for molecular imaging

Siddiqui

Kadlecek

Pourfathi

et al. 2017

Advanced Drug Delivery Reviews

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Until recently, molecular imaging using magnetic resonance (MR) has been limited by the modality’s low sensitivity, especially with non-proton nuclei. The advent of hyperpolarized (HP) MR overcomes this limitation by substantially enhancing the signal of certain biologically important probes through a process known as external nuclear polarization, enabling real-time assessment of tissue function and metabolism. The metabolic information obtained by HP MR imaging holds significant promise in the clinic, where it could play a critical role in disease diagnosis and therapeutic monitoring. This review will provide a comprehensive overview of the developments made in the field of hyperpolarized MR, including advancements in polarization techniques and delivery, probe development, pulse sequence optimization, characterization of healthy and diseased tissues, and the steps made towards clinical translation.

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On the present and future of dissolution-DNP

Cited by 221 publications

References 47 publications

Influence of Dy3+ and Tb3+ doping on 13C dynamic nuclear polarization

Influence of Dy3+ and Tb3+ doping on 13C dynamic nuclear polarization

The effect of glassing solvent deuteration and Gd³⁺ doping on ¹³C DNP at 5 T

The use of hyperpolarized carbon-13 magnetic resonance for molecular imaging

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On the present and future of dissolution-DNP

Cited by 221 publications

References 47 publications

Influence of Dy3+ and Tb3+ doping on 13C dynamic nuclear polarization

Influence of Dy3+ and Tb3+ doping on 13C dynamic nuclear polarization

The effect of glassing solvent deuteration and Gd3+ doping on 13C DNP at 5 T

The use of hyperpolarized carbon-13 magnetic resonance for molecular imaging

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The effect of glassing solvent deuteration and Gd³⁺ doping on ¹³C DNP at 5 T