On the Reaction of Sex Hormones and Related Compounds With Metals in Ethanolic Solutions by Spectrophotometry

Itabashi, Hiroko

doi:10.1507/endocrj1954.7.284

Cited by 4 publications

(2 citation statements)

References 2 publications

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Materials and MethodsMetals tested were Cu, Ni, Co, Mn, Fe (Fe++ and Fe+++) in the form of their sulphates, except Fe+++ which was used in the form of iron alum. Ultraviolet absorption spectra were measured by HITACHI Model ESP-2 A spectrophotometer, equipped with quartz cuvettes, 1cm thickness.

In order to make the effect of metals on the spectra of the steroids, the absorption spectra of ethanol, ethanol with metal, ethanolic solution of the steroids and of the steroid solution with metal were determined.

Given volume of ethanol was placed in the Cuvett I and II, and the same volume of an ethanolic solution of the steroids was put in the Cuvett III.

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On the interaction of progestins with metals in ethanolic solution

Itabashi¹

1968

Nihon Chikusan Gakkaiho

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The author and co-worker (ITABASHI and ISHIDA, 1957) found the highest concentration of copper in the swine ovary during estrus.The author (ITABASHI, 1959(ITABASHI, , 1960 also found that estrogen and androgen were able to form a complex with metals including copper, judging from a significant shift of their wave length of maximum absorption in ethanolic solution after addition of the metals.In the present experiment, the reaction of progestin and related compounds with metals was studied. Materials and MethodsMetals tested were Cu, Ni, Co, Mn, Fe (Fe++ and Fe+++) in the form of their sulphates, except Fe+++ which was used in the form of iron alum. Ultraviolet absorption spectra were measured by HITACHI Model ESP-2 A spectrophotometer, equipped with quartz cuvettes, 1cm thickness.In order to make the effect of metals on the spectra of the steroids, the absorption spectra of ethanol, ethanol with metal, ethanolic solution of the steroids and of the steroid solution with metal were determined.Given volume of ethanol was placed in the Cuvett I and II, and the same volume of an ethanolic solution of the steroids was put in the Cuvett III. The absorption spectra of the Cuvett II and III were determined (Sp II & Sp III, respectively) at the range between 215 and and III, and the absorption of these cuvetts (Sp IIm & Sp IIIm, respectively) were determined at the same range described previously.Difference in the absorption spectrum obtained between Sp IIIm-Sp III and Sp IIm-Sp II was used for a criterion of the formation of the steroids and the metal complexes.

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Given volume of ethanol was placed in the Cuvett I and II, and the same volume of an ethanolic solution of the steroids was put in the Cuvett III.

…”

mentioning

confidence: 99%

“…The author (ITABASHI, 1959(ITABASHI, , 1960 also found that estrogen and androgen were able to form a complex with metals including copper, judging from a significant shift of their wave length of maximum absorption in ethanolic solution after addition of the metals.…”

mentioning

confidence: 99%

On the interaction of progestins with metals in ethanolic solution

Itabashi¹

1968

Nihon Chikusan Gakkaiho

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Implications of reiterative DNA—Metal ion complexes in the induction and development of neoplastic cells

Guillé

Grisvard

Sissoëff

1979

Biol Trace Elem Res

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Experimental data on the content in metal ions of DNA preparations from various neoplastic and healthy tissues are summarized: metal ions are preferentially bound to reiterative DNA sequences, where they may induce conformational variations and thus modify the binding of effector molecules such as repressors and polymerases.A model is described where essential and toxic metals are successively loaded on ligand acceptor groups of increasing affinity and thus may reach the final active sites: enzymes and reiterative DNA sequences (equated at least partially to regulative DNA sequences). The effects of some molecules, including peptides, antibiotics, growth factors, hormones, and antineoplastic substances, on DNA conformation could be explained in part by their chelating ability.The neoplastic state may be induced by a modification of metal ion transfer chains: quantitatively by a continuous derepression of genes coding for metal ligands, genes that are only temporarily derepressed during development in normal cells, and qualitatively by modifications of the nucleotidic sequence of structural genes leading to an increase of the chelating ability of the coded metal ligand.

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