On the Role of the Stable Plasminogen Activator of the Venous Wall in Occlusion Induced Fibrinolytic State

R, Constantini; Spöttl, F; Holzknecht, F; Braunsteiner, H

doi:10.1055/s-0038-1651390

Cited by 7 publications

(5 citation statements)

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“…In a previous study on venous occlusion in humans it was found that 20 min of stasis produced a shift in PA activity from the adventitial to the intimal layer (15). In our study no such signs were detected.…”

Section: Discussioncontrasting

confidence: 56%

Effect of Venous Stasis on Vessel Wall Fibrinolysis

1984

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SummaryThe effects of prolonged venous stasis on release of plasminogen activators (PA) from the caval vein of rats were studied.PA activity was continuously reduced in the intimal layer and after 8 hr of stasis the reduction was statistically significant. After 2 and 3 days no intimal PA activity was found. The PA activity in the adventitial vasa vasorum was unchanged during the experimental period.

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Section: Discussioncontrasting

confidence: 56%

Effect of Venous Stasis on Vessel Wall Fibrinolysis

1984

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METHODS

Five adult full grown rabbits weighing 2.5 to 3.5 kg were used.

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mentioning

confidence: 99%

The Effect of Experimental Arterial Occlusion On the Plasminogen Tissue Activator in Rabbits

R¹,

Hilbe²,

Holzknecht³

et al. 1973

Angiology

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Venous occlusion seems to be a potent stimulus to provoke an enhancement of fibrinolytic activity in plasma and in venous endothelium, 2,3,4,5,6,7 In addition Ogston et a1.8 reported on a significantly increased plasminogen activator of the venous blood compared with the arterial blood. Therefore we tried to elucidate the role of tissue activator of the arterial wall in experimental arterial occlusion. METHODSFive adult full grown rabbits weighing 2.5 to 3.5 kg were used. The proximal part of the arteria femoralis was prepared and ligated for two hours. Immediately proximal and distal of the ligature a piece of 5 mm length was taken. Controls were taken from the contralateral artery. The fibrinolytic activity was assayed by the histochemical method of Todd9 as modified by Pandolfi.l° Rabbit-fibrinogen was prepared by the method of Astrup and Mullertz.ll RESULTSThe endothelium of the unligated arteries was found to be completely inactive. The pieces of arteries taken immediately distal of the ligature showed a high endothelial lysis (see Fig. 1). Proximal of the ligature 1 rabbit showed one single microscopical pin-hole only, another one a complete endothelial lysis, whereas the endothelium of three other rabbits was completely inactive (see Table 1). The fibrinolytic activity of the adventitia was located around the vasa vasorum and did not differ significantly in all three groups. DISCUSSIONIn rabbits aorta Astrup and Buluk 12 found no fibrinolytic activity using the KSCN-extraction method. Whereas Warren 13 showed endothelial lysis in frozen &dquo;hdutchen&dquo; preparations using Todd's histochemical method.9 These results may support the theory that mechanical lesions of endothelial cells cause a release of plasminogen activator from the endothelium. Springate et a1.14 injected a suspension of clot emboli into the ear vein of rabbits, and found that approximately 90% of clot-emboli disappear from the pulmonary arterial vasculature within the first four hours. Barnardls injected fibrin-clots into earveins of rabbits and drew blood after V2, 1, 2, 6, and 24 hours. No fibrinolytic activity in rabbit's blood was found. These experiments 14,1' indicate that the From the department of Internal Medicine (Prof. Dr. H. Braunsteiner) and from the department of Surgery (Prof.

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“…This assay method has proved useful in comparison of the fibrinolytic activity of human veins of arms and legs in healthy persons and patients with thrombosis (11 , 12, 13). The same principle, but modified, has been used by other authors to assess the fibrinolytic activity of veins (3,4) and of the prostata (7).…”

Section: Resultsmentioning

confidence: 99%

Technical Remarks on the Microscopical Demonstration of Tissue Plasminogen Activator

Pandolfi¹,

Bjernstad²,

Nilsson³

1972

Thromb Haemost

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SummaryThe authors examine some variables capable of influencing the results obtained with the fibrin slide technique for microscopical demonstration of fibrinolytic activators in tissues (Todd’s method).The speed of freezing of the tissue specimen proved to be especially important. Slow freezing of the specimen should be avoided since it results in sections of poor quality and may alter the distribution of fibrinolytic activity. The optimal thickness of the sections lies between 6 and 8 (jim. The concentration of fibrinogen used as substrate may vary between 0.7 and 2.0% and the thickness of the fibrin film between 0.07 and 0.21 mm. Increasing the temperature of incubation from +4° C to +37° C enhanced fibrinolysis, but had no effect on the distribution of fibrinolytic activity in the sections. Storage of the sections at —20° C and at +4° C for 6 days had no apparent effect on their fibrinolytic activity. Storage at 28° C resulted in a considerable loss of the fibrinolytic activity of the sections. The error of a method to assess the strength of fibrinolytic activity of the section is determined.

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On the Role of the Stable Plasminogen Activator of the Venous Wall in Occlusion Induced Fibrinolytic State

Cited by 7 publications

References 0 publications

Effect of Venous Stasis on Vessel Wall Fibrinolysis

Effect of Venous Stasis on Vessel Wall Fibrinolysis

The Effect of Experimental Arterial Occlusion On the Plasminogen Tissue Activator in Rabbits

Technical Remarks on the Microscopical Demonstration of Tissue Plasminogen Activator

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