On the selective killing of cold atmospheric plasma cancer treatment: Status and beyond

Yan, Dayun; Horkowitz, Alex; Wang, Qihui

doi:10.1002/ppap.202100020

Cited by 15 publications

(8 citation statements)

References 150 publications

(299 reference statements)

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“…For in vitro studies, a medium layer is necessary for experimental design and is responsible for most observed cellular responses after CAP treatment, particularly for the cases involving CAP-treated solutions or media [40,46]. (5) CAP shows selective killing effect on cancer cell lines compared to their counterpart normal cell lines in many cases [47].…”

Section: Refmentioning

confidence: 99%

Preclinical Cold Atmospheric Plasma Cancer Treatment

Limanowski

Yan

Lin

et al. 2022

Cancers

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CAP is an ionized gas generated under atmospheric pressure conditions. Due to its reactive chemical components and near-room temperature nature, CAP has promising applications in diverse branches of medicine, including microorganism sterilization, biofilm inactivation, wound healing, and cancer therapy. Currently, hundreds of in vitro demonstrations of CAP-based cancer treatments have been reported. However, preclinical studies, particularly in vivo studies, are pivotal to achieving a final clinical application. Here, we comprehensively introduced the research status of the preclinical usage of CAP in cancer treatment, by primarily focusing on the in vivo studies over the past decade. We summarized the primary research strategies in preclinical and clinical studies, including transdermal CAP treatment, post-surgical CAP treatment, CAP-activated solutions treatment, and sensitization treatment to drugs. Finally, the underlying mechanism was discussed based on the latest understanding.

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Section: Refmentioning

confidence: 99%

Preclinical Cold Atmospheric Plasma Cancer Treatment

Limanowski

Yan

Lin

et al. 2022

Cancers

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“…The frequency of U87MG is higher than the value of hTERT/E6/E7, which means that Mode 1 (the most contribution mode) of U87MG has a closer frequency to the plasma treatment discharge frequency at 12.5 kHz. This may explain the selectivity where more U87MG cells can be killed than the hTERT/E6/E7 by the electromagnetic emissions from the plasma where the discharge frequency has a bandwidth due to the particle thermal velocities . In another previous work, one can find a higher sensitivity of hTERT/E6/E7 to chemical treatment, causing more apoptosis of it compared with U78MG.…”

Section: Resultsmentioning

confidence: 81%

“…This may explain the selectivity where more U87MG cells can be killed than the hTERT/E6/E7 by the electromagnetic emissions from the plasma where the discharge frequency has a bandwidth due to the particle thermal velocities. 43 In another previous work, one can find a higher sensitivity of hTERT/E6/ E7 to chemical treatment, causing more apoptosis of it compared with U78MG. At the same time, the fact that only the physical emissions from the plasma can kill more hTERT/ E6/E7 is confirmed.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Cell Membrane Oscillations under Radiofrequency Electromagnetic Modulation

et al. 2023

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Cell responses to external radiofrequencies (RF) are a fundamental problem of much scientific research, clinical applications, and even daily lives surrounded by wireless communication hardware. In this work, we report an unexpected observation that the cell membrane can oscillate at the nanometer scale in phase with the external RF radiation from kHz to GHz. By analyzing the oscillation modes, we reveal the mechanism behind the membrane oscillation resonance, membrane blebbing, the resulting cell death, and the selectivity of plasma-based cancer treatment based on the difference in the membrane's natural frequencies among cell lines. Therefore, a selectivity of treatment can be achieved by aiming at the natural frequency of the target cell line to focus the membrane damage on the cancer cells and avoid normal tissues nearby. This gives a promising cancer therapy that is especially effective in the mixing lesion of the cancer cells and normal cells such as glioblastoma where surgical removal is not applicable. Along with these new phenomena, this work provides a general understanding of the cell coupling with RF radiation from the externally stimulated membrane behavior to the cell apoptosis and necrosis.

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“…9–12 However, the working principles of plasma-induced anticancer remain unclear due to the lack of specifically designed plasma exposure devices, hindering its further clinical application. 13…”

Section: Introductionmentioning

confidence: 99%