On the spectrum of the generalized Gelfand pair $(u(p,q), h_{n})$, $p+q=n$

Godoy, T.; Saal, Linda

doi:10.7146/math.scand.a-15113

Cited by 3 publications

(3 citation statements)

References 7 publications

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“…Since hepatotoxicity is one of the main reasons for drugs to be retracted from the market, a reliable platform for pre-clinical liver toxicity screening is an ideal tool to reduce costs. The human HepaRG® lines an attractive tool for a first quick screening of new chemical entities 32,33 . The HepaRG® cell line is derived from a human hepatocarcinoma.…”

Section: Design Of Experimentsmentioning

confidence: 99%

Metabolomics profiling of steatosis progression in HepaRG ® cells using sodium valproate

et al. 2018

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Non-alcoholic Fatty Liver Disease (NAFLD) is a frequently encountered Drug-Induced Liver Injury (DILI). Although this stage of the disease is reversible, it can lead to irreversible damage provoked by non-alcoholic steatohepatitis (NASH), fibrosis and cirrhosis. Therefore, the assessment of NAFLD is a paramount objective in toxicological screenings of new drug candidates. In this study, a metabolomic fingerprint of NAFLD induced in HepaRG cells at four dosing schemes by a reference toxicant, sodium valproate (NaVPA), was obtained using liquid-liquid extraction followed by liquid chromatography and accurate mass-mass spectrometry (LC-AM/MS). The combination of a strict design of experiment with a robust detection method, applied on sodium valproate, validated the possibilities of untargeted metabolomics in hepatic toxicological research. Distinctive patterns between exposed and control cells were consistently observed, multivariate analyses selected up to 200 features of interest, revealing hallmark NAFLD-biomarkers, such as diacylglycerol and triglyceride accumulation and carnitine deficiency. Initial toxic responses show increased levels of S-adenosylmethionine and mono-acetylspermidine in combination with only a moderate increase in triglycerides. New specific markers of toxicity have been observed, such as spermidines, creatine, and acetylcholine. The described design of experiment provides a valuable metabolomics platform for mechanistic research of toxicological hazards and identified new markers for steatotic progression.

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Section: Design Of Experimentsmentioning

confidence: 99%

Metabolomics profiling of steatosis progression in HepaRG ® cells using sodium valproate

et al. 2018

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“…As a result, concentration gradients of substances (growth factors, metabolites, and oxygen) are formed, which are dissolved in the blood or secreted by the overlying periportal hepatocytes [34]. The gradient is determined by changes in the composition of blood plasma and oxygenation differences in the bloodstream of the area between the periportal and perivenous zones [35]. The phenomenon of hepatocyte heterogeneity has been studied in many works [34][35][36][37][38].…”

Section: Discussionmentioning

confidence: 99%

“…The gradient is determined by changes in the composition of blood plasma and oxygenation differences in the bloodstream of the area between the periportal and perivenous zones [35]. The phenomenon of hepatocyte heterogeneity has been studied in many works [34][35][36][37][38]. It was shown that hepatocytes located in the periportal zone (zone 1) specialize in gluconeogenesis and β-oxidation, while glycolysis, lipogenesis, and detoxification processes of high intensity were detected in hepatocytes located around the central vein (zone 3).…”

Section: Discussionmentioning

confidence: 99%

Multiphoton Microscopy and Mass Spectrometry for Revealing Metabolic Heterogeneity of Hepatocytes in vivo

Rodimova¹,

Kuznetsova²,

Bobrov³

et al. 2021

Sovrem Tehnol Med

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The aim of the investigation was to study the possibility of revealing the heterogeneity of normal liver hepatocytes in terms of metabolic status using the modern methods of multiphoton microscopy and mass spectrometry.Materials and Methods. Heterogeneity of hepatocytes in terms of total metabolic activity was assessed using multiphoton microscopy based on the autofluorescence intensity of intracellular cofactors NAD(P)H and FAD. Hepatocyte heterogeneity in terms of intensity of intracellular metabolic processes was determined using the fluorescence lifetime imaging (FLIM) method based on the data about fluorescence lifetime contributions of various forms of NAD(P)H. The method of time-of-flight secondary ion mass spectrometry (TоF-SIMS) was used to study the lipid and amino acid composition of hepatocytes.Results. It has been revealed using multiphoton microscopy that hepatocytes are heterogeneous in terms of general metabolic activity. Using FLIM, it was established that the heterogeneity degree was high in terms of intensity of oxidative phosphorylation, glycolysis,

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Some spherical analysis related to the pairs (U(n), H n ) and (U(p,q), H n ), p + q = n

Campos

Saal

2015

Proc Math Sci

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On the spectrum of the generalized Gelfand pair $(u(p,q), h_{n})$, $p+q=n$

Abstract: It is known that the spectrum of the Gelfand pair $(U(n), H_{n})$ is homeomorphic to the Heisenberg fan. In this paper after defining a suitable notion of spectrum, we prove an analogous result for the generalized Gelfand pair $(U(p,q), H_{n})$, $p+q=n$.

Cited by 3 publications

References 7 publications

Metabolomics profiling of steatosis progression in HepaRG ® cells using sodium valproate

Metabolomics profiling of steatosis progression in HepaRG ® cells using sodium valproate

Multiphoton Microscopy and Mass Spectrometry for Revealing Metabolic Heterogeneity of Hepatocytes in vivo

Some spherical analysis related to the pairs (U(n), H n ) and (U(p,q), H n ), p + q = n

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