On the structure of rhinovirus 1A

Medappa, K. C.; McLean, C; Rueckert, Roland R.

doi:10.1016/0042-6822(71)90258-3

Cited by 109 publications

(96 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar effects were obserVed in protozoan cultures (6). The general conclusion from the work reported here, namely that the trichothecenes are inhibitors of eukaryotic protein synthesis, was established directly by Ueno and colleagues (7) (12) at 370 with orbital shaking in glassware treated with Siliclad (ClayAdams, Parsippany, N.J.). For experiments involving the incorl)oratioll of radioactive leucine into nascent proteins, cells were harvested at 10,000 X g for 5 mim in a Sorvall RC-2B centrifuge and resuspended in a similar medium AL (13) lacking amino acids and serum.…”

supporting

confidence: 61%

Mechanism of Inhibition of Eukaryotic Protein Synthesis by Trichothecene Fungal Toxins

Cundliffe

Cannon

Davies

1974

Proc. Natl. Acad. Sci. U.S.A.

206

120

View full text Add to dashboard Cite

show abstract

supporting

confidence: 61%

Mechanism of Inhibition of Eukaryotic Protein Synthesis by Trichothecene Fungal Toxins

Cundliffe

Cannon

Davies

1974

Proc. Natl. Acad. Sci. U.S.A.

206

120

View full text Add to dashboard Cite

show abstract

“…BMV RNA was isolated from purified virus by phenol extraction (11). RNA 4, RNA 3, and a mixture of RNA 1 and 2 were obtained by fractionation of whole BMV RNA on 5-20% sucrose density gradients centrifuged in a Spinco SW25 rotor at 25,000 rpm for 18 hr followed by a second separation of appropriately combined fractions (6).…”

Section: Methodsmentioning

confidence: 99%

Translation of Brome Mosaic Viral Ribonucleic Acid in a Cell-Free System Derived from Wheat Embryo

Shih

Kaesberg

1973

Proc. Natl. Acad. Sci. U.S.A.

145

View full text Add to dashboard Cite

The four RNAs of brome mosaic virus induce substantial incorporation of amino acids into protein when used as messengers in a cell-free proteinsynthesizing system derived from wheat embryo. RNA showed, quite unequivocally, that viral coat protein can be synthesized in a cell-free system from wheat embryo programmed with the RNA of satellite tobacco necrosis virus (STNV). The in vitro product has nearly the same spectrum of tryptic peptides as authentic STNV coat protein, although it is slightly lower in molecular weight, presumably as a consequence of an aberrant post-translational proteolytic cleavage. Klein et al. presented evidence that translation is initiated correctly and that synthesis of the single product is substantial. The exceptional success of this in vitro synthesis is attributable to the careful definition, by these investigators and also by Marcus and his colleagues (4, 5), of several of the important variables of the wheat system.The efficacy of the wheat-embryo system, the partial success of brome mosaic virus (BMV) RNA and STNV RNA translation in Escherichia coli extracts (6-8), and the fact that wheat is a natural host of BMV (although not of STNV) has prompted us to test the BMV RNAs as messengers in a wheat-embryo system. We find that the BMV RNAs serve as excellent messengers-with an efficiency comparable to that reported for bacteriophage RNA in E. coli extracts.BMV is one of several RNA-containing plant and animal viruses whose genetic complement is divided among several nucleic acids (9 MATERIALS AND METHODSPreparation of BMV, BMV RNA, and BMV Coat Protein. BMV (Russian strain) was grown and isolated by procedures described (10). BMV RNA was isolated from purified virus by phenol extraction (11). RNA 4, RNA 3, and a mixture of RNA 1 and 2 were obtained by fractionation of whole BMV RNA on 5-20% sucrose density gradients centrifuged in a Spinco SW25 rotor at 25,000 rpm for 18 hr followed by a second separation of appropriately combined fractions (6).Coat protein was prepared by dialyzing the virus against 1 M CaCI2. This degrades the virus and quantitatively precipitates the viral RNA, leaving the protein in solution (12).Isolation of Wheat Embryo and Preparation of an S23 CellFree Extract. Wheat embryos were isolated from seeds of Kenosha winter wheat (Triticum vulgare L, cultivar kenosha, 1799 Abbreviations: STNV, satellite tobacco necrosis virus; BMV, brome mosaic virus.

show abstract

“…Two h later the infected cells were harvested, washed and lysed in NET buffer containing 1% NP40 as described in the preceding section. Capsid polypeptides were prepared from poliovirus particles purified according to a modified procedure of Medappa et al (1971). Naturally occurring procapsids were isolated from the crude extracts of infected cells by centrifugation (Beckman rotor SW25.1; 24 000 rev/min, 4 h) through a sucrose concentration gradient (15 to 30%, w/w).…”

Section: Viruses and Cellsmentioning

confidence: 99%

Antigenic and Structural Relatedness among Non-capsid and Capsid Polypeptides of Polioviruses belonging to Different Serotypes

Romanova

Tolskaya

Agol

1981

Journal of General Virology

View full text Add to dashboard Cite

SUMMARYAntibodies were raised by immunization of Macaca fascicularis monkeys with extracts of M. fascicularis kidney cells separately infected with one of the three poliovirus serotypes. Preparations of antibodies were shown to be strictly type-specific in the neutralization test but formed immune complexes adsorbable on Staphylococcus aureus, Cowan I strain cells, with heterotypic as well as homotypic virus-specific polypeptides present in extracts from the virus-infected cells. The presence of intertypic antigenic determinants was demonstrated by this technique on both the non-capsid and capsid poliovirus polypeptides.Structural variations of poliovirus polypeptides were studied by analysing products of their partial proteolysis. Non-capsid polypeptides encoded in the central portion of the virus genome (polypeptides 5b and X) as well as in the 3'-terminal region (NCVP2 and NCVP4) were found to be highly conserved, whereas capsid polypeptides VP1, VP2, and VP3, which are encoded in the 5'-terminal region of the virus RNA, displayed a much greater variability.

show abstract

On the structure of rhinovirus 1A

Cited by 109 publications

References 34 publications

Mechanism of Inhibition of Eukaryotic Protein Synthesis by Trichothecene Fungal Toxins

Mechanism of Inhibition of Eukaryotic Protein Synthesis by Trichothecene Fungal Toxins

Translation of Brome Mosaic Viral Ribonucleic Acid in a Cell-Free System Derived from Wheat Embryo

Antigenic and Structural Relatedness among Non-capsid and Capsid Polypeptides of Polioviruses belonging to Different Serotypes

Contact Info

Product

Resources

About