On the use of ultrafiltration for the concentration and desalting of phosvitin from egg yolk protein concentrate

Abstract

An ultrafiltration-based approach was integrated in the preparation of phosvitin (PVs) from delipidated egg yolk proteins. An attempt was made to concentrate PVs as well as to desalt by means of the diafiltration technique. Primary experiments were devoted to optimise the ultrafiltration performance as function of parameters such as the effects of pH, feed concentration and transmembrane pressure on permeate flux with the 10-kDa molecular weight cut-off (MWCO) polyethersulfone membrane at laboratory scale. Higher permeate flux values were observed at low concentration and at alkaline pH, whatever transmembrane pressure studied. Then, desalting of PVs was carried out at 50°C with 10-and 30-kDa MWCO membranes. The results showed that desalting of PVs was obtained with both the 10-and 30-kDa MWCO membranes and with a few loss of protein in the permeate side.

Combination of High Hydrostatic Pressure and Ultrafiltration to Generate a New Emulsifying Ingredient from Egg Yolk

“…The permeation fluxes of both plasmas were not significantly different at each VCF (p > 0.05). At 1×, the permeation flux was 29 L/m 2 •h, which is lower than values results presented by Chay Pak Ting et al [45] (50 L/m 2 •h) during UF (10 or 30 kDa) of crude phosphvitin solutions (5.0% w/v), with another cross-flow UF system. As the feed was concentrated, the permeation flux decreased to 20 L/m 2 •h at 2×, but remained stable until the end of UF (VCF = 5×).…”

“…As the feed was concentrated, the permeation flux decreased to 20 L/m 2 •h at 2×, but remained stable until the end of UF (VCF = 5×). Adversely, the permeation fluxes observed by Chay Pak Ting et al [45] decreased by 48% at a VCF of 6×. In the latter study, however, UF was conducted at 50 • C, which possibly increased membrane fouling incidence due to a lower calcium phosphate solubility at this temperature.…”

“…UF experiments were adapted from Chay Pak Ting et al [45] A laboratory scale tangential flow filtration (TFF) system (Millipore Sigma, Oakville, ON, Canada) was used with a PES membrane having a molecular weight cut-off (MWCO) of 10 kDa and a membrane surface of 0.005 m 2 (Biomax ® membrane, Millipore Sigma, Oakville, ON, Canada). Following the first membrane cleaning, the pure water flux was of 288 L/m 2 •h.…”

“…To the best of our knowledge, only Chay Pak Ting et al [45] investigated the use of polyethersulfone (PES) ultrafiltration (UF) membrane (molecular weight cut-off [MWCO] of 10 and 30 kDa) for the concentration and purification of phosvitin. Fractionation by UF (50 • C, volume concentration factor (VCF) of 6×) and diafiltration (DF) (10 diavolumes, VCF of 11×) produced a phosvitin recovery rate of 84% in the retentate.…”

Comparative Composition and Antioxidant Activity of Peptide Fractions Obtained by Ultrafiltration of Egg Yolk Protein Enzymatic Hydrolysates

“…Prior to the hydrolysis reaction, EYP and PPP were rehydrated in 0.1 N NaOH (5%, w/v) and partially dephosphorylated by incubating the solution at 37 °C for 3 h, as previously described [25]. …”