New Zealand Dairy cows are predominantly milked twice a day (TAD), however full-season once a day (OAD) milking was practiced by ~9% of herds in New Zealand in the season 2015-2016 (Edwards, 2018). OAD milking offers benefits to both the cow and farmer and has been detailed previously by Stelwagen et al. (2013). Changing from TAD to OAD causes a reduction in milk volume and introduces changes in milk composition. A positive effect of OAD is a significant increase in the content of valuable bioactive molecules, such as lactoferrin (LF), and other immune-related proteins in milk, such as acute phase proteins and RNases (Farr et al., 2002; Stelwagen et al., 2011). LF is produced in bovine milk as part of a wide range of bioactive proteins and peptides, which have roles in immunity and immunoregulation functions (Galfi et al., 2016). LF has been found to have antibacterial activity for bacteria with high iron requirements like Escherichia coli and Staphylococcus aureus, coagulase negative Staphylococcus sp. and Pseudomonas aeruginosa (González-Chávez, Arévalo-Gallegos, & Rascón-Cruz, 2009). In addition, LF exhibits antiviral activity to both DNA and RNA viruses, and antifungal activity against Candida sp., Aspergillus sp. and Trichophyton sp. (González-Chávez et al., 2009). Lactoferrin has been investigated for the last 20 years for its ability to stimulate immune response by inducing granulopoiesis, participating in the process of cytokines and antibodies synthesis in vitro, activating complement and producing interleukins (IL-1 & IL-2) and tumor necrosis factor D (Legrand, 2011). In addition, LF was characterized as a factor intervening in the fight of cancer (Duarte, Nicolau,