Onchocerca volvulus heat shock protein 70 is a major immunogen in amicrofilaremic individuals from a filariasis-endemic area

Rothstein, Neil; Higashi, Gene I.; Yates, John L.; Rajan, T. V.

doi:10.1016/0166-6851(89)90084-4

Cited by 56 publications

(23 citation statements)

References 13 publications

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“…Not only are the HSPs of Plasmodium falciparum antigenic, but T-cells reactive to the P. falciparum HSP70-1 protein have been described (Behr et al 1992). HSPs from the helminths Schistosoma mansoni (Johnson et al 1989) and Onchocerca volvulus (Rothstein et al 1989) are antigenic. Antibodies and T-cells from patients with autoimmune diseases have recognized HSPs from several infectious agents, including Mycobacteria (Rambukkana et al 1993).…”

Section: Discussionmentioning

confidence: 99%

Antigenicity of Trichomonas vaginalis heat-shock proteins in human infections

Davis-Hayman

Shah

Finley

et al. 2000

Parasitology Research

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Patients infected with Trichomonas vaginalis mount humoral and cellular immune responses that often do not protect against reinfection. The oxidative stressors produced by leukocytes may trigger a heat-shock-like response in T. vaginalis trophozoites, helping the parasite to survive host immune defenses. The antigenicity of T. vaginalis heat-shock proteins (HSPs) was examined by immunoprecipitation of T. vaginalis heat-induced proteins with sera from infected patients and controls. When T. vaginalis was heat-shocked, HSPs of 169-167 and 140-137 kDa were specifically recognized by sera from infected male and female patients. However, the majority of T. vaginalis HSPs were also immunoprecipitated by control sera; all sera recognized 72- to 71-kDa, 47- to 45-kDa, 38- to 37-kDa, 35-kDa, and 31-kDa heat-induced proteins. At least 15 proteins from non-heat-shocked T. vaginalis were immunoprecipitated by sera from infected patients and controls, indicating that natural or cross-reacting antibodies could participate in host responses to trichomoniasis. Molecules of 158, 135, 89, and 74-72 kDa were immunoprecipitated from some non-heat-shocked parasites only by patients' sera. A 38-kDa T. vaginalis protein was immunoprecipitated only by sera from infected females and may be specific for infection in women.

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Section: Discussionmentioning

confidence: 99%

Antigenicity of Trichomonas vaginalis heat-shock proteins in human infections

Davis-Hayman

Shah

Finley

et al. 2000

Parasitology Research

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“…There are a number of Hsps: large Hsps such as Hsp60, Hsp70, and Hsp90, as well as small Hsps with molecular masses of 12–43 kDa [16], [17]. Several Hsps have been characterized in parasitic nematodes: Hsp20 in N. brasiliensis [18]; Hsp20 and Hsp70 in Haemonchus contortus [19], [20]; Hsp70 in Onchocerca volvulus and Parastrongyloides trichosuri [21], [22]; and Hsp12.6, Hsp18, and Hsp90 in Brugia spp. [23]–[25].…”

Section: Introductionmentioning

confidence: 99%

Hsp12.6 Expression Is Inducible by Host Immunity in Adult Worms of the Parasitic Nematode Nippostrongylus brasiliensis

Arizono

Yamada²,

Tegoshi³

et al. 2011

PLoS ONE

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Heat shock proteins (Hsp) are a family of stress-inducible molecular chaperones that play multiple roles in a wide variety of animals. However, the roles of Hsps in parasitic nematodes remain largely unknown. To elucidate the roles of Hsps in the survival and longevity of nematodes, particularly at the 2 most critical stages in their lifecycle, the infective-L3 stage and adult stage, which is subjected to host-derived immunological pressure, we examined the temporal gene transcription patterns of Hsp12.6, Hsp20, Hsp70, and Hsp90 throughout the developmental course of the nematode Nippostrongylus brasiliensis by reverse transcriptase real-time PCR. Nb-Hsp70 and Nb-Hsp90 expression were observed throughout the nematode's lifecycle, while the expression of Nb-Hsp20 was restricted to adults. Interestingly, Nb-Hsp12.6 showed a biphasic temporal expression pattern; i.e., it was expressed in infective-L3 larvae and in adults during worm expulsion from immunocompetent rats. However, the activation of Nb-Hsp12.6 in adult worms was aborted when they infected permissive athymic-rnu/rnu rats and was only marginal when they infected mast-cell-deficient Ws/Ws rats, which exhibited a low response of rat mast cell protease (RMCP) II and resistin-like molecule (Relm)- β expression compared to those observed in immunocompetent rats. Moreover, the activation of Nb-Hsp12.6 was reversed when adult worms were transplanted into the naive rat intestine. These features of Nb-Hsp12.6, the expression of which is not only stage-specific in infective-L3, but is also inducible by mucosal immunity in adults, have implications for the survival strategies of parasitic nematodes in deleterious environmental conditions both outside and inside the host.

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“…BCG is widely used as a vaccine against tuberculosis, and a large number of people are sensitized to mycobacteria or other parasites through natural contacts. Moreover, sensitization to HSPs has been reported to occur in individuals naturally exposed to a wide variety of infections, such as malaria (24), filariasis (34,37), schistosomiasis (27), American trypanosomiasis (12), leprosy (9,25), and candidiasis (17). It has also been reported that vaccination against pertussis using a whole-cell vaccine is capable of priming the immune system to respond to microbial HSPs (10).…”

Section: Discussionmentioning

confidence: 99%

Exposure to Mycobacteria Primes the Immune System for Evolutionarily Diverse Heat Shock Proteins

Qazi

Julián

et al. 2005

Infect Immun

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During stress conditions, such as infection, the synthesis of heat shock proteins (HSPs) in microorganisms is upregulated. Since a high degree of homology exists within each HSP family, we postulated that exposure to microorganisms could prime the immune system for evolutionarily diverse HSPs. We tested this hypothesis by priming mice with three microorganisms, namely, Mycobacterium bovis BCG, Mycobacterium vaccae, and Chlamydia pneumoniae. After this, mice received a dose of the various HSPs. We found that BCG and M. vaccae but not C. pneumoniae primed the immune system for the induction of secondary immunoglobulin G (IgG) responses to most of the HSPs tested. Analysis of the IgG1 and IgG2a profile and gamma interferon production induced against the HSPs revealed the induction of a mixture of responses. We also observed that sera from mice treated with M. vaccae and HSP70 were cross-reactive, but no antibody complexes were observed in their kidneys, which frequently are targets for autoantibody reactions. Our findings add further support for the use of HSPs as effective vaccine adjuvants.

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Onchocerca volvulus heat shock protein 70 is a major immunogen in amicrofilaremic individuals from a filariasis-endemic area

Cited by 56 publications

References 13 publications

Antigenicity of Trichomonas vaginalis heat-shock proteins in human infections

Antigenicity of Trichomonas vaginalis heat-shock proteins in human infections

Hsp12.6 Expression Is Inducible by Host Immunity in Adult Worms of the Parasitic Nematode Nippostrongylus brasiliensis

Exposure to Mycobacteria Primes the Immune System for Evolutionarily Diverse Heat Shock Proteins

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