Oncogenic Activity of Wrap53 in Human Colorectal Cancer In Vitro and in Nude Mouse Xenografts

Abstract

BackgroundWD40-encoding RNA antisense to p53 (Wrap53) has been implicated in cancer development. However, the role of Wrap53 remains unknown in colorectal cancer. The aim of this study was to elucidate the function of Wrap53 in colorectal cancer tumorigenesis and development.Material/MethodsThis study analyzed Wrap53 expression in colorectal cancer tissue specimens using The Cancer Genome Atlas data and tumor cell lines and assessed the effects of Wrap53 knockdown on regulation of cancer cell malignant phenotypes in vitro and in nude mouse xenografts.ResultsWrap53 expression was upregulated in colorectal cancer tissue specimens and cell lines. Knockdown of Wrap53 expression induced colorectal cancer cell line apoptosis and cell cycle arrest in the G1 phase, but reduced tumor cell line proliferation and invasion in vitro. Knockdown of Wrap53 in a nude mouse xenograft assay inhibited tumor cell line xenograft formation and growth.ConclusionsWrap53 is likely a potential oncogene or possesses oncogenic activity in colorectal cancer, promoting colorectal tumorigenesis. Targeting Wrap53 expression may represent a novel strategy for the control of colorectal cancer.

The roles of long noncoding RNAs in breast cancer metastasis

“…1 ). (1) Intergenic lncRNAs originate from DNA sequences between two protein-coding genes, such as MALAT1 13 , Linc-ROR 14 , and ANCR 15 ; (2) intronic lncRNAs originate from introns of protein-coding genes, such as PRUNE2 16 , PCA3 17 , COLDAIR 18 , and SYISL 19 ; (3) sense lncRNAs overlap with one or more introns and exons of different protein-coding genes, such as LncRNA-GAS5 13 and ecCEPBA 20 ; (4) antisense lncRNAs originate from an opposite direction to a protein-coding gene, such as TALAM1 21 , PDCD4-AS1 22 , and Wrap53 23 ; (5) enhancer lncRNAs originate from the regions of promoter enhancer, such as LncRNA-LEENE 24 and Lnc-SLC4A1-1 25 ; and (6) bidirectional lncRNAs exist in proximity of a coding transcript of the opposite strand, such as Linc00441 26 and Catsper1au 27 . So far, lncRNAs have been shown to exert auxiliary functions either to tumor suppression or to tumorigenesis.…”

Differential Effects of WRAP53 Transcript Variants on the Biological Behaviours of Human Non-Small Cell Lung Cancer Cells

“…Previous studies indicated that WRAP53β was a potential oncoprotein, whose overexpression led to transformation and promoted cancer cell survival, and whose downregulation induced massive cell death [29][30][31][32]. In addition, overexpression of WRAP53β was related to NSCLC progression.…”

Evaluation of dyskerin expression and the Cajal body protein WRAP53β as potential prognostic markers for patients with primary vaginal carcinoma

“…Loss of WRAP53β is associated with poor prognosis in head and neck (26), breast (27) and ovarian cancer (28), suggesting a tumour suppressor role (29,30). Although upregulation of WRAP53β has also been reported in cancer, correlation with patient survival has been inconsistent (31)(32)(33). Instead, this upregulation, which is known to occur in precancerous lesions, may reflect WRAP53β involvement in DNA repair in order to constrain tumour progression (29,30).…”