Oncogenic miR‑425‑5p is associated with cellular migration, proliferation and apoptosis in renal cell carcinoma

Quan, Jing; Li, Yawen; Pan, Xiang; Lai, Yulin; He, Tao; Lin, Cheng-Yuan; Zhou, Liang; Zhao, Liwen; Sun, Shuai; Ding, Yu; Tao, Lingzhi; Hu, Yimin; Wu, Xiaohong; Chen, Zebo; Zhang, Fangting; Ye, Jing; Ni, Liangchao; Lai, Yongqing

doi:10.3892/ol.2018.8948

Cited by 23 publications

(28 citation statements)

References 30 publications

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“…Previous reports have indicated that miRNAs serve a regulatory role in cell proliferation, differentiation, apoptosis, migration and metabolism (12,24,25). Studies have revealed that enhanced expression levels of miR-425-5p exist in different types of cancer, which suggest that miR-425-5p may have multiple functions in the development of cancer (22,26). Studies previously demonstrated that miR-425-5p can promote invasion and metastasis in hepatocellular carcinoma, colorectal cancer and gastric cancer (27)(28)(29).…”

Section: Discussionmentioning

confidence: 99%

Downregulation of microRNA‑425‑5p suppresses cervical cancer tumorigenesis by targeting AIFM1

Zhang¹,

Yang²,

Liu³

et al. 2019

Exp Ther Med

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Although microRNA-425-5p (miR-425-5p) has been previously revealed to be upregulated in cervical cancer, the cellular function of miR-425-5p in cervical cancer remains unknown. The aim of the current study was to investigate the cellular function of miR-425-5p and its underlying mechanism in cervical cancer. Reverse transcription-quantitative polymerase chain reaction was used to measure miR-425-5p expression in several cervical cancer cell lines. TargetScan bioinformatics analysis was used to predict apoptosis-inducing factor mitochondria-associated 1 (AIFM1) as a novel target of miR-425-5p, and this was verified by dual-luciferase reporter assay. Furthermore, cell transfections were used to investigate the role of miR-425-5p in cervical cancer. The effect of miR-425-5p on cell viability and apoptosis in HeLa cells was detected by MTT assay and flow cytometry, respectively. The present study demonstrated that miR-425-5p was significantly upregulated in cervical cancer cell lines. In addition, AIFM1 was identified as a direct target of miR-425-5p and negatively regulated by miR-425-5p. Downregulation of miR-425-5p inhibited HeLa cell viability and induced cell apoptosis. Furthermore, downregulation of miR-425-5p significantly increased the protein and mRNA expression levels of cytochrome c, caspase-3, caspase-9 and DNA damage regulated autophagy modulator 1. The effects of miR-425-5p inhibition on HeLa cell viability and apoptosis were significantly reversed by AIFM1 knockdown. In conclusion, the present study demonstrated that miR-425-5p was upregulated in cervical cancer, and downregulation of miR-425-5p inhibited cervical cancer cell growth by targeting AIFM1.

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Section: Discussionmentioning

confidence: 99%

Downregulation of microRNA‑425‑5p suppresses cervical cancer tumorigenesis by targeting AIFM1

Zhang¹,

Yang²,

Liu³

et al. 2019

Exp Ther Med

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“…6,7 Emerging evidence suggests that miRNAs are abnormally expressed in human cancers, 8,9 and can function as either tumor suppressors or oncogenes, depending on the target genes regulated. 8,9 miRNAs were also reported to modulate many essential cell behaviors including cell proliferation, invasion, and apoptosis. 8,9 miR-152 is a member of the miR-148/152 family that shows reduced expression patterns in various types of cancer.…”

Section: Introductionmentioning

confidence: 99%

“…8,9 miRNAs were also reported to modulate many essential cell behaviors including cell proliferation, invasion, and apoptosis. 8,9 miR-152 is a member of the miR-148/152 family that shows reduced expression patterns in various types of cancer. 10 miR-152 functions as a tumor suppressor by targeting genes in cancers including nasopharyngeal carcinoma, endometrial cancer, and ovarian cancer.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: miR-152 inhibits the proliferation and invasion of chordoma cells by targeting HOXC8

Fang

Yan

2019

J Int Med Res

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Objective MicroRNAs (miRNAs) are non-coding RNAs that affect the expression of their target genes by binding to the 3′-untranslated region. miR-152 has been identified as a critical modulator in tumorigenesis, but its role in chordoma has not been explored. We therefore investigated the role of miR-152 in regulating chordoma cell behavior, and examined the downstream effectors of miR-152. Materials and methods We examined the expression of miR-152 in two human chordoma cell lines and in a normal human embryonic kidney cell line. We also analyzed the relationship between miR-152 and homeobox C8 ( HOXC8) by bioinformatics analysis and luciferase reporter assay. We determined the effects of miR-152 and HOXC8 expression on chordoma cell behavior. Results miR-152 expression was downregulated in chordoma compared with normal cells. Meanwhile, miR-152 overexpression inhibited chordoma cell proliferation and invasion. The oncogene HOXC8 was a direct target of miR-152, as shown by luciferase reporter and western blot assays. Conclusions HOXC8 acted as an effector for the suppressive role of miR-152 in chordoma, thereby providing a potential therapeutic target in patients with chordoma.

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“…miR‐103 can reduce the phosphorylation of Yes‐associated protein (YAP), which is a regulator of the Hippo pathway and plays an important role in cell proliferation and tumour progression . miR‐425 has been suggested to be an oncogene in several types of cancer, including renal and hepatocellular carcinoma . The expression of miR‐140 is decreased in breast cancer stem cells (BCSCs), and miR‐140 acts as a tumour suppressor gene to inhibit tumour proliferation by regulating Wnt1 .…”

Section: Discussionmentioning

confidence: 99%

“…24 miR-425 has been suggested to be an oncogene in several types of cancer, including renal and hepatocellular carcinoma. 25,26 The expression of miR-140 is decreased in breast cancer stem cells (BCSCs), and miR-140 acts as a tumour suppressor gene to inhibit tumour proliferation by regulating Wnt1. 27 It has been reported that miR-185 expression is downregulated in some tumours and that upregulation of miR-185 expression significantly inhibits the growth, migration, and invasion of Ewing's sarcoma cells.…”

Section: Discussionmentioning

confidence: 99%

Serum exosomal microRNAs as novel biomarkers for multiple myeloma

Zhang

Geng

et al. 2019

Hematological Oncology

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Accumulating studies have focused on circulating microRNAs, which might be potential biomarkers for different malignancies. The aim of this study was to investigate the potential of serum exosomal microRNAs to be novel serum biomarkers for smouldering myeloma (SMM) or even multiple myeloma (MM). The levels of serum exosomal microRNAs and serum circulating microRNAs were measured in healthy individuals and patients with SMM (n = 20) or MM (n = 20). Serum exosomal microRNAs and serum circulating microRNAs were extracted from serum, and the expression levels of selected microRNAs were quantified by real‐time polymerase chain reaction (PCR). The levels of serum exosome‐derived miR‐20a‐5p, miR‐103a‐3p, and miR‐4505 were significantly different among patients with MM, patients with SMM, and healthy individuals, while there were differences in the levels of let‐7c‐5p, miR‐185‐5p, and miR‐4741 in patients with MM relative to those in SMM patients or healthy controls. Additionally, a significant correlation was rarely found between the levels of serum and exosomal microRNAs. This study shows that serum exosomal microRNAs can be used independently as novel serum biomarkers for MM.

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Oncogenic miR‑425‑5p is associated with cellular migration, proliferation and apoptosis in renal cell carcinoma

Cited by 23 publications

References 30 publications

Downregulation of microRNA‑425‑5p suppresses cervical cancer tumorigenesis by targeting AIFM1

Downregulation of microRNA‑425‑5p suppresses cervical cancer tumorigenesis by targeting AIFM1

RETRACTED: miR-152 inhibits the proliferation and invasion of chordoma cells by targeting HOXC8

Serum exosomal microRNAs as novel biomarkers for multiple myeloma

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