Oncolytic adenoviruses in anticancer therapy: Current status and prospects

Svyatchenko, V. A.; Tarasova, M. V.; Нетесов, С. В.; Chumakov, P. M.

doi:10.1134/s0026893312040103

Cited by 12 publications

(8 citation statements)

References 107 publications

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“…Nucleic acids are attractive compounds for modulating the cell function with high precision but their poor efficacy in crossing the cell-protective plasma membrane limit their ability to reach the intracellular site of action [1][2][3][4][5]. Modified viruses are on the frontline of DNA delivery for gene therapy [6,7] but they cannot directly deliver synthetic versions of mRNA, siRNA or oligonucleotides which may exhibit superior activity and minimal immune or toxic response. Some synthetic nucleic acids, administrated alone, were shown to alleviate muscle [8] or liver pathologies [9] but only a small portion of the injected nucleic acids still reaches the cell cytoplasm and/or nucleus.…”

Section: Introductionmentioning

confidence: 99%

Self-aggregating 1.8 kDa polyethylenimines with dissolution switch at endosomal acidic pH are delivery carriers for plasmid DNA, mRNA, siRNA and exon-skipping oligonucleotides

Chiper

Tounsi

Kole

et al. 2017

Journal of Controlled Release

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Efficiency of polyethylenimine (PEI) for nucleic acid delivery is affected by the size of the carrier and length of the nucleic acids. For instance, PEIs with molecular weights between 10-30kDa provide optimal DNA delivery activity whereas PEIs with molecular weights below 1.8kDa are ineffective. The activity of PEI is also severely diminished by substitution of DNA for shorter nucleic acids such as mRNA or siRNA. Here, through chemical modification of the primary amines to aromatic domains we achieved nucleic acid delivery by the 1.8kDa polyethylenimine (PEI) particles. This modification did not affect the PEI buffering abilities but enhanced its pH-sensitive aggregation, enabling stabilization of the polyplex outside the cell while still allowing nucleic acid release following cellular entry. The aromatic PEIs were then evaluated for their gene, mRNA, siRNA and 2'O-methyl phosphorothioate oligonucleotide in vitro transfection abilities. The salicylamide-grafted PEI showed to be a reliable carrier for delivering nucleic acids with cytoplasmic activity such as the mRNA and siRNA or nuclear diffusible oligonucleotide. It was then further equipped with polyethyleneglycol (PEG) and the delivery efficiency of the copolymer was tested in vivo for regeneration of dystrophin in the muscle of mdx mouse through a 2'O-methyl phosphorothioate-mediated splicing modulation. Intramuscular administration of polyplexes resulted in dystrophin-positive fibers in a mouse model of Duchenne muscular dystrophy without apparent toxicity. These findings indicate that precise modifications of low molecular weight PEI improve its bio-responsiveness and yield delivery vehicles for nucleic acids of various types in vitro and in vivo.

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Section: Introductionmentioning

confidence: 99%

Self-aggregating 1.8 kDa polyethylenimines with dissolution switch at endosomal acidic pH are delivery carriers for plasmid DNA, mRNA, siRNA and exon-skipping oligonucleotides

Chiper

Tounsi

Kole

et al. 2017

Journal of Controlled Release

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“…There are several reported incidences about the cytotoxicity of OVs, some of which resulted in fatality. Svyatchenko reported leukemia developed in two volunteers after being part of a clinical trial for an OV‐based gene therapy that aimed to treat inherited immune disorder called X‐linked severe combined immunodeficiency (SCID). Another healthy volunteer was reported to be killed due to toxic shock in a clinical trial for the treatment of inherited disorder called ornithine transcarbamylase deficiency using adenovirus .…”

Section: Uniqueness Of Aptamers As Targeting Elementsmentioning

confidence: 99%

“…Svyatchenko reported leukemia developed in two volunteers after being part of a clinical trial for an OV‐based gene therapy that aimed to treat inherited immune disorder called X‐linked severe combined immunodeficiency (SCID). Another healthy volunteer was reported to be killed due to toxic shock in a clinical trial for the treatment of inherited disorder called ornithine transcarbamylase deficiency using adenovirus . Therefore, the therapeutic applications of OVs are often limited in cancer or gene therapy due to their adverse side effects and cancer resurgence rate as well as their potential in inducing immune responses, tumorigenicity, and insertional mutagenesis .…”

Section: Uniqueness Of Aptamers As Targeting Elementsmentioning

confidence: 99%

“…Another healthy volunteer was reported to be killed due to toxic shock in a clinical trial for the treatment of inherited disorder called ornithine transcarbamylase deficiency using adenovirus . Therefore, the therapeutic applications of OVs are often limited in cancer or gene therapy due to their adverse side effects and cancer resurgence rate as well as their potential in inducing immune responses, tumorigenicity, and insertional mutagenesis . They are also associated with other disadvantages including low gene capacity, low viral transduction, and high immunogenicity …”

Section: Uniqueness Of Aptamers As Targeting Elementsmentioning

confidence: 99%

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Advancing Aptamers as Molecular Probes for Cancer Theranostic Applications—The Role of Molecular Dynamics Simulation

Jeevanandam

Tan

Danquah

et al. 2020

Biotechnology Journal

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Theranostics cover emerging technologies for cell biomarking for disease diagnosis and targeted introduction of drug ingredients to specific malignant sites. Theranostics development has become a significant biomedical research endeavor for effective diagnosis and treatment of diseases, especially cancer. An efficient biomarking and targeted delivery strategy for theranostic applications requires effective molecular coupling of binding ligands with high affinities to specific receptors on the cancer cell surface. Bioaffinity offers a unique mechanism to bind specific target and receptor molecules from a range of non‐targets. The binding efficacy depends on the specificity of the affinity ligand toward the target molecule even at low concentrations. Aptamers are fragments of genetic materials, peptides, or oligonucleotides which possess enhanced specificity in targeting desired cell surface receptor molecules. Aptamer–target binding results from several inter‐molecular interactions including hydrogen bond formation, aromatic stacking of flat moieties, hydrophobic interaction, electrostatic, and van der Waals interactions. Advancements in Systematic Evolution of Ligands by Exponential Enrichment (SELEX) assay has created the opportunity to artificially generate aptamers that specifically bind to desired cancer and tumor surface receptors with high affinities. This article discusses the potential application of molecular dynamics (MD) simulation to advance aptamer‐mediated receptor targeting in targeted cancer therapy. MD simulation offers real‐time analysis of the molecular drivers of the aptamer‐receptor binding and generate optimal receptor binding conditions for theranostic applications. The article also provides an overview of different cancer types with focus on receptor biomarking and targeted treatment approaches, conventional molecular probes, and aptamers that have been explored for cancer cells targeting.

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“…in parvoviruses [ 4 ], or double stranded, e.g. in oncolytic adenoviruses [ 5 ] and poxviruses [ 6 ]. The genomic RNA of oncolytic viruses can also have different forms: positive sense single- stranded RNA (enteroviruses [ 7 ]), double stranded RNA (reoviruses [ 8 ]), or negative sense single-stranded RNA (paramyxoviruses and rhabdoviruses in [ 9 ]).…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of Oncolysis by Paramyxovirus Sendai

Matveeva¹,

Кочнева

Нетесов

et al. 2015

Acta Naturae

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Some viral strains of the Paramyxoviridae family may be used as anti-tumor agents. Oncolytic paramyxoviruses include attenuated strains of the measles virus, Newcastle disease virus, and Sendai virus. These viral strains, and the Sendai virus in particular, can preferentially induce the death of malignant, rather than normal, cells. The death of cancer cells results from both direct killing by the virus and through virus-induced activation of anticancer immunity. Sialic-acid-containing glycoproteins that are overexpressed in cancer cells serve as receptors for some oncolytic paramyxoviruses and ensure preferential interaction of paramyxoviruses with malignant cells. Frequent genetic defects in interferon and apoptotic response systems that are common to cancer cells ensure better susceptibility of malignant cells to viruses. The Sendai virus as a Paramyxovirus is capable of inducing the formation of syncytia, multinuclear cell structures which promote viral infection spread within a tumor without virus exposure to host neutralizing antibodies. As a result, the Sendai virus can cause mass killing of malignant cells and tumor destruction. Oncolytic paramyxoviruses can also promote the immune-mediated elimination of malignant cells. In particular, they are powerful inducers of interferon and other cytokynes promoting antitumor activity of various cell components of the immune response, such as dendritic and natural killer cells, as well as cytotoxic T lymphocytes. Taken together these mechanisms explain the impressive oncolytic activity of paramyxoviruses that hold promise as future, efficient anticancer therapeutics.

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Oncolytic adenoviruses in anticancer therapy: Current status and prospects

Cited by 12 publications

References 107 publications

Self-aggregating 1.8 kDa polyethylenimines with dissolution switch at endosomal acidic pH are delivery carriers for plasmid DNA, mRNA, siRNA and exon-skipping oligonucleotides

Self-aggregating 1.8 kDa polyethylenimines with dissolution switch at endosomal acidic pH are delivery carriers for plasmid DNA, mRNA, siRNA and exon-skipping oligonucleotides

Advancing Aptamers as Molecular Probes for Cancer Theranostic Applications—The Role of Molecular Dynamics Simulation

Mechanisms of Oncolysis by Paramyxovirus Sendai

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