Oncostatin M inhibits differentiation of rat stem Leydig cells in vivo and in vitro

Abstract

Oncostatin M (OSM) is a pleiotropic cytokine within the interleukin six family of cytokines, which regulate cell growth and differentiation in a wide variety of biological systems. However, its action and underlying mechanisms on stem Leydig cell development are unclear. The objective of the present study was to investigate whether OSM affects the proliferation and differentiation of rat stem Leydig cells. We used a Leydig cell regeneration model in rat testis and a unique seminiferous tubule culture system after ethane dimethane sulfonate (EDS) treatment to assess the ability of OSM in the regulation of proliferation and differentiation of rat stem Leydig cells. Intratesticular injection of OSM (10 and 100 ng/testis) from post‐EDS day 14 to 28 blocked the regeneration of Leydig cells by reducing serum testosterone levels without affecting serum luteinizing hormone and follicle‐stimulating hormone levels. It also decreased the levels of Leydig cell‐specific mRNAs (Lhcgr, Star, Cyp11a1, Hsd3b1, Cyp17a1 and Hsd11b1) and their proteins by the RNA‐Seq and Western blotting analysis. OSM had no effect on the proliferative capacity of Leydig cells in vivo. In the seminiferous tubule culture system, OSM (0.1, 1, 10 and 100 ng/mL) inhibited the differentiation of stem Leydig cells by reducing medium testosterone levels and downregulating the expression of Leydig cell‐specific genes (Lhcgr, Star, Cyp11a1, Hsd3b1, Cyp17a1 and Hsd11b1) and their proteins. OSM‐mediated action was reversed by S3I‐201 (a STAT3 antagonist) or filgotinib (a JAK1 inhibitor). These data suggest that OSM is an inhibitory factor of rat stem Leydig cell development.

Aspirin inhibits adipogenesis of tendon stem cells and lipids accumulation in rat injury tendon through regulating PTEN/PI3K/AKT signalling

“…rMATS.42 Principle Component Analysis was used to detect Alternative splicing events and plots, and gene expression level was used for correlation analysis. The differentially expressed genes in R, Python or shell environment for statistical computing and graphics were used to perform Hierarchical Clustering, Gene Ontology, scatter plots, volcano plots and pathway analysis …”

Aspirin promotes tenogenic differentiation of tendon stem cells and facilitates tendinopathy healing through regulating the GDF7/Smad1/5 signaling pathway

“…rMATS.42 principle component analysis was used to detect alternative splicing events and plots, and the gene expression level was used for correlation analysis. The differentially expressed genes in R, Python, or shell environment for statistical computing and graphics were used to perform Hierarchical Clustering, Gene Ontology, scatter plots, volcano plots, and pathway analysis (Wang, Xie, et al, ).…”

Gestational vinclozolin exposure suppresses fetal Leydig cell development in rats

“…We performed RNA-seq of the testis and biological pathway analysis to address the pathway of VCZmediated action in vivo as previously described [28]. Gene expression level, Gene Ontology, Pathway analysis, scatter plots, and volcano plots were performed.…”

“…Western blot was performed as previously described [28]. In brief, testis is lysed, denatured, electrophoresed and blotted with the primary antibody followed by the secondary antibody.…”