One-chip electronic detection of DNA hybridization using precision impedance-based CMOS array sensor

Lee, Kang-Ho; Lee, Jeong-Oen; Sohn, Mi‐Jin; Lee, Byunghun; Choi, Seibum B.; Kim, Sang Kyu; Yoon, Jun‐Bo; Cho, Gyu‐Hyeong

doi:10.1016/j.bios.2010.07.055

Cited by 39 publications

(21 citation statements)

References 23 publications

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“…It is composed of a comparator, 12-bit counters, and a shift register. In this AID conversion, highly accurate estimates of the output can be obtained through counting during a long time interval [8,16].…”

Section: Operating Principlementioning

confidence: 99%

“…1. An equivalent capacitor is formed using two bio-modified electrodes: a probe-immobilized metal electrode and a quasi-electrode in the electrolyte [8]. The capacitive biosensor measures the changes in the capacitance, which is caused by the changes in the dielectric properties and/or the thickness of the dielectric layer at the electrode-solution interface due to the probe-target molecule binding.…”

Section: Introductionmentioning

confidence: 99%

“…This method can estimate the capacitance with a low complexity compared with other capacitive sensors [4,[12][13][14]. However, there are several issues that degrade the sensor sensitivity, such as the DC drift in the electrode potential that is caused by a mismatch between the excitation currents [9], the offset in the extracted capacitance of interest due to the electrode voltage charged initially [9,10], and the weakness against common-mode noises, such as power-supply noise or substrate coupling noise [8][9][10][11]. These issues may cause large standard deviations and poor reproducibility of the measured signal, thereby limiting the performance of the sensor.…”

Section: Introductionmentioning

confidence: 99%

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An alternating current-driven control for biomolecule detection

Lee

2015

2015 15th International Conference on Control, Automation and Systems (ICCAS)

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A biosensor with an alternating current-driven control is realized for label-free detection of biomolecule. This sensor precisely measures the capacitive changes in the electrode-electrolyte interface after hybridization with complementary DNA targets. Conventional capacitive biosensors generally use the excitation method with double current-sources. However these sensors have limitations due to performance-based issues of the DC potential drift and offsets, as well as weakness against common-mode noises. In this paper, the proposed device includes differential electrode pairs that are located in a single current-source line with sensing circuits, which results in the elimination of the effect due to the current mismatch. The device also shows an improved signal-to-noise ratio. Additionally, the individual control of the switch controller in this device offers robust and reproducible measurement capabilities independent of the duty asymmetry and pre-charged initial offset voltage. The device is a hybrid sensor that has both working electrodes and readout circuitry in one-chip using a CMOS process. The prototype successfully demonstrated the specific detection of oligonucleotide sequences. The device consumes 2.34mW from a 3.3 V supply in a 0.35 I-lm CMOS process.

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Section: Operating Principlementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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An alternating current-driven control for biomolecule detection

Lee

2015

2015 15th International Conference on Control, Automation and Systems (ICCAS)

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“…(2) Figure 3(a) gives access to both cell constants in the bulk and in the sensitive area, and (4). The bulk and fluid capacitances and the fluid resistance are given by (5). The height of the resolution domain is set to be twice the cell width to enclose 99% of the electric energy [9].…”

Section: Interdigitated Sensor Structure the Structure View Is Illusmentioning

confidence: 99%

“…The results in terms of fluid impedance are extracted out of the modulus and the phase of the response. A more effective way to stimulate the sensor is the use of a triangular signal (Triangular Waveform Voltage (TWV)) as described in [4] and applied to DNA hybridization detection [5]. In this paper, the proposed sensor interface implements the principle of a triangular wave voltage generation by a square current signal.…”

Section: Introductionmentioning

confidence: 99%

A Self-Oscillating System to Measure the Conductivity and the Permittivity of Liquids within a Single Triangular Signal

Druart¹,

Flandre²,

Francis

2014

Journal of Sensors

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We present a methodology and a circuit to extract liquid resistance and capacitance simultaneously from the same output signal using interdigitated sensing electrodes. The principle consists in the generation of a current square wave and its application to the sensor to create a triangular output voltage which contains both the conductivity and permittivity parameters in a single periodic segment. This concept extends the Triangular Waveform Voltage (TWV) signal generation technique and is implemented by a system which consists in a closed-loop current-controlled oscillator and only requires DC power to operate. The system interface is portable and only a small number of electrical components are used to generate the expected signal. Conductivities of saline NaCl and KCl solutions, being first calibrated by commercial equipment, are characterized by a system prototype. The results show excellent linearity and prove the repeatability of the measurements. Experiments on water-glycerol mixtures validate the proposed sensing approach to measure the permittivity and the conductivity simultaneously. We discussed and identified the sources of measurement errors as circuit parasitic capacitances, switching clock feedthrough, charge injection, bandwidth, and control-current quality.

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A Peptide Nucleic Acid (PNA)‐DNA Ferrocenyl Intercalator for Electrochemical Sensing

et al. 2016

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1IntroductionDNA-based biosensors have been involved in aw ide range of applications such as medicald iagnosis [1],a gricultural and food sciences [2],g ene sequences analysis [3] and environmental hazards control [4] using different transduction modes such as light sensitive, frequency, electronic and electrochemical based techniques [5][6][7]. Amongt hem, electrochemicalD NA biosensors present an attractiver oute for synergizing the biological recognition process and the transduction event, with the advantages of af ast, specific, sensitive,a nd inexpensive detection system. In order to make such devices viable, researchers continuously try to develop methods that simplify the sensor constructiona nd improve its sensitivity, selectivity and robustness.An electrochemical DNAs ensor is typically based on the binding ability of an ucleic acid layer with ac omplementary target DNAs equence.V arious types of oligonucleotides have been usedb yr esearchers as probe molecules to selectively capture aD NA target;t hese probes includes ingle-stranded DNA[ 8],h airpin DNA[ 9], locked nucleic acid (LNA) [10] and peptiden ucleic acid (PNA) [11],w hich are grafted to the substrate surface via physical or electrochemical adsorption, self-assembly immobilization, biotin-avidin interactions, entrapment methods or covalent attachment [12,13].In the past years,n umerous strategies have been suggested for the development of electrochemicalD NA sensors [14],i ncludingl abel-free methods directly based on inherent electroactivity of some nucleotides [15,16],a nd indirectl abel-based methods which involvet he use of electroactive markersa nd labels such as enzymes,n ano-particles,q uantum dots,a nd redox indicators and intercalators mainly derived from organic dyes,a nticancer or antibiotic drugs and metal complexes [17][18][19][20].O ft hese types of configurations,t he widely adoptedo nes employ electroactive indicators containing organometallic or organic moieties,w hich connect directly and selectively to the oligonucleotide sequences by electrostatic interactions, groove binding or intercalating interaction [19b]. Such intercalators,d efined as organic smalls ized DNA binding molecules with an appropriate chemical structure, e.g. methylene blue,d aunomycin, anthraquinone,e thidium bromide and ferrocenyln aphthalenediimide [20][21][22][23][24], present characteristic structures with ap lanar polycyclic ring, allowingt hem to fit between adjacentb ase pairs of Abstract:Aferrocenyl intercalator was investigated to developa ne lectrochemical DNAb iosensor employing ap eptide nucleic acid (PNA) sequencea sc apture probe. After hybridizationw ith single strand DNAs equence, anaphthalene diimide intercalator bearing ferrocene moieties (FND)w as introduced to bind with the PNA-DNA duplex and the electrochemical signalo ft he ferrocene molecules was used to monitor the DNAr ecognition. Electrochemical impedance spectroscopyw as used to characterizet he different modification steps.D ifferential pulse voltammetry was employed to evaluate...

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One-chip electronic detection of DNA hybridization using precision impedance-based CMOS array sensor

Cited by 39 publications

References 23 publications

An alternating current-driven control for biomolecule detection

An alternating current-driven control for biomolecule detection

A Self-Oscillating System to Measure the Conductivity and the Permittivity of Liquids within a Single Triangular Signal

A Peptide Nucleic Acid (PNA)‐DNA Ferrocenyl Intercalator for Electrochemical Sensing

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