One-dimensional acoustic standing waves in rectangular channels for flow cytometry

Suthanthiraraj, Pearlson P. A.; Piyasena, Menake E.; Woods, Travis A.; Naivar, Mark A.; López, Gabriel P.; Graves, Steven W.

doi:10.1016/j.ymeth.2012.02.013

Cited by 47 publications

(60 citation statements)

References 35 publications

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“…3, 35,[49][50][51][52][53][54] In this paper, measurements performed in two glass capillaries are presented to show, respectively, the transducer-plane streaming and the modal Rayleigh-like streaming in layered acoustofluidic particle manipulation devices. The two glass capillaries (Vitrocom, USA) have inner dimensions of 0.3 × 6 mm 2 and 2 × 6 mm 2 (h × w) and wall thicknesses of 0.3 mm and 0.8 mm, respectively.…”

Section: Methodsmentioning

confidence: 99%

Modal Rayleigh-like streaming in layered acoustofluidic devices

2016

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Classical Rayleigh streaming is well known and can be modelled using Nyborg’s limiting velocity method as driven by fluid velocities adjacent to the walls parallel to the axis of the main acoustic resonance. We have demonstrated previously the existence and the mechanism of four-quadrant transducer plane streaming patterns in thin-layered acoustofluidic devices which are driven by the limiting velocities on the walls perpendicular to the axis of the main acoustic propagation. We have recently found experimentally that there is a third case which resembles Rayleigh streaming but is a more complex pattern related to three-dimensional cavity modes of an enclosure. This streaming has vortex sizes related to the effective wavelength in each cavity axis of the modes which can be much larger than those found in the one-dimensional case with Rayleigh streaming. We will call this here modal Rayleigh-like streaming and show that it can be important in layered acoustofluidic manipulation devices. This paper seeks to establish the conditions under which each of these is dominant and shows how the limiting velocity field for each relates to different parts of the complex acoustic intensity patterns at the driving boundaries.

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Section: Methodsmentioning

confidence: 99%

Modal Rayleigh-like streaming in layered acoustofluidic devices

2016

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“…33 While the device shown herein only requires materials and methods used in conventional microfabrication, we emphasize that there are a handful of other techniques that can be used for constructing similar devices. 19,34,35 The advantages of this approach include its simplicity as well as the durability of the final device.…”

Section: Discussionmentioning

confidence: 99%

“…We note that other acoustofluidic devices that support bulk acoustic standing waves can be fabricated from glass or quartz capillaries bound to piezoelectric transducers, which is described elsewhere. 19,20 Silicon-based devices offer the advantages of robustness and control over the flow channel geometry, which together allow for numerous types of processing for samples containing suspensions of particles and cells. The devices are reusable provided they are properly cleaned between uses (i.e., by flushing the device with buffers and detergents).…”

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confidence: 99%

Fabrication and Operation of Acoustofluidic Devices Supporting Bulk Acoustic Standing Waves for Sheathless Focusing of Particles

Shields

Cruz

Ohiri

et al. 2016

JoVE

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Acoustophoresis refers to the displacement of suspended objects in response to directional forces from sound energy. Given that the suspended objects must be smaller than the incident wavelength of sound and the width of the fluidic channels are typically tens to hundreds of micrometers across, acoustofluidic devices typically use ultrasonic waves generated from a piezoelectric transducer pulsating at high frequencies (in the megahertz range). At characteristic frequencies that depend on the geometry of the device, it is possible to induce the formation of standing waves that can focus particles along desired fluidic streamlines within a bulk flow. Here, we describe a method for the fabrication of acoustophoretic devices from common materials and clean room equipment. We show representative results for the focusing of particles with positive or negative acoustic contrast factors, which move towards the pressure nodes or antinodes of the standing waves, respectively. These devices offer enormous practical utility for precisely positioning large numbers of microscopic entities (e.g., cells) in stationary or flowing fluids for applications ranging from cytometry to assembly.

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“…Goddard et al 19 evaluated the performance of a flow cytometer that used acoustic energy to focus particles/cells to the center of a flow stream. Suthanthiraraj et al 20 presented the one-dimensional acoustic standing waves for focusing of flowing cells and particles in a rectangular flow channel. Jakobsson et al 21 also introduced a method to focus and orient red blood cells by using the ultrasonic standing waves on a microfluidic chip.…”

Section: Introductionmentioning

confidence: 99%

A novel microfluidic flow focusing method

Jiang

Weng

2014

Biomicrofluidics

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A new microfluidic method that allows hydrodynamic focusing in a microchannel with two sheath flows is demonstrated. The microchannel network consists of a T-shaped main channel and two T-shaped branch channels. The flows of the sample stream and the sheath streams in the microchannel are generated by electroosmotic flow-induced pressure gradients. In comparison with other flow focusing methods, this novel method does not expose the sample to electrical field, and does not need any external pumps, tubing, and valves. V C 2014 AIP Publishing LLC.

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One-dimensional acoustic standing waves in rectangular channels for flow cytometry

Cited by 47 publications

References 35 publications

Modal Rayleigh-like streaming in layered acoustofluidic devices

Modal Rayleigh-like streaming in layered acoustofluidic devices

Fabrication and Operation of Acoustofluidic Devices Supporting Bulk Acoustic Standing Waves for Sheathless Focusing of Particles

A novel microfluidic flow focusing method

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