One‐Dimensional <scp>SDS</scp> Gel Electrophoresis of Proteins

Gallagher, Sean R.

doi:10.1002/0471140856.txa03fs32

Cited by 13 publications

(1 citation statement)

References 13 publications

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“…1.14.4). Gallagher, 2007) Figure 1.14.4 Western blotting analysis and gene expression for evaluation the effects of exposure to free TiO 2 nanoparticles. (A) The metallothionein (MT) level in untreated samples and in larvae exposed to free TiO 2 nanoparticles was evaluated.…”

Section: Detection Of Protein Expression By Western Blottingmentioning

confidence: 99%

Metallic Nano‐Composite Toxicity Evaluation by Zebrafish Embryo Toxicity Test with Identification of Specific Exposure Biomarkers

et al. 2017

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Nanomaterials (NM) have different shapes and can be composed of different materials such as carbon, silicon, and some metals like gold, silver, and titanium. They are used as fillers, catalysts, semiconductors, cosmetics, drug carriers in medicine, energy storage systems, and antifriction coatings. NM are the parent compounds of nanoparticles (NPs), which may be divided into two groups: fullerenes and engineered nanoparticles (ENPs). After crossing the cell membrane, NPs may be stored in vesicles, mitochondria, and additional organelles within epithelial cells. They may generate reactive oxygen species, oxidative stress, and cytotoxicity, as well as denaturation of proteins, apoptosis, and necrosis. Nowadays, new toxicological data are required to assess the potential exposure of the environment and human beings to pollutants. The aim of the present investigation is to evaluate the toxicity of the metallic nano-composite by the zebrafish embryo toxicity test (ZFET). The methods described here can be easily adapted to other nanocomposites or nanomaterials with some variations. © 2017 by John Wiley & Sons, Inc.

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Section: Detection Of Protein Expression By Western Blottingmentioning

confidence: 99%

Metallic Nano‐Composite Toxicity Evaluation by Zebrafish Embryo Toxicity Test with Identification of Specific Exposure Biomarkers

et al. 2017

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Measuring p66Shc Signaling Pathway Activation and Mitochondrial Translocation in Cultured Cells

et al. 2015

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The adaptor protein p66Shc links membrane receptors to intracellular signaling pathways, with downstream consequences on mitochondrial metabolism and reactive oxygen species production. Moreover, p66Shc has also been implicated in cancer development, progression, and metastasis. Increased phosphorylation of serine 36 residue of p66Shc very often correlates with oxidative stress-associated pathologies. The pro-oxidative role of p66Shc also appears to be involved in chemical toxicity, being an important component of stress responses triggered by xenobiotics. Here, we present a protocol that can be used: (a) for isolation of mitochondrial, cytosolic, and mitochondrial-associated membrane fractions from adherent cells lines; (b) to perform p66Shc detection with specific antibodies in order to monitor its translocation between different cellular compartments in response to the oxidative stress; and (c) to modulate the p66Shc pathway with the use of pharmacological approaches or gene-silencing methods.

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PROTAC-Induced Proteolytic Targeting

Carmony

Kim

2012

Methods in Molecular Biology

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Summary Small molecule modulators of protein activity are increasingly being utilized as tools to examine the functional roles of proteins. Operating at the post-translational level, these molecules provide enhanced temporal and spatial control and mitigate the potential for compensatory responses in comparison with classical genetic approaches. Proteolysis targeting chimeric molecules, or PROTACs, are small molecules that inhibit the function of their target proteins by targeting them for degradation by the ubiquitin proteasome system (UPS). This chapter summarizes strategies for PROTAC preparation and characterization.

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One‐Dimensional SDS Gel Electrophoresis of Proteins

Cited by 13 publications

References 13 publications

Metallic Nano‐Composite Toxicity Evaluation by Zebrafish Embryo Toxicity Test with Identification of Specific Exposure Biomarkers

Metallic Nano‐Composite Toxicity Evaluation by Zebrafish Embryo Toxicity Test with Identification of Specific Exposure Biomarkers

Measuring p66Shc Signaling Pathway Activation and Mitochondrial Translocation in Cultured Cells

PROTAC-Induced Proteolytic Targeting

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